LOCUS       sequence-337870-       10190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of Luciferase cDNA with GFP as a 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.Luciferase.IRES.GFP
FEATURES             Location/Qualifiers
     source          1..10190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        57..248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        267..493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             494..674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    721..846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1339..1572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1757..1801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2014..2131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2189..2595
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2636..4285
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2702..2720)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    join(4324..4898,4899..4901,4902..4910)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4491..4508)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4718..4737
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4911..4913,4914..4916,4917..5630)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4911..4913,4914..4916,4917..5627)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4956..4977)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5217..5236)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5564..5585
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5646..6234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5699..5719)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6117..6128)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6306..6539
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6617..6738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6654..6673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6708..6727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6778..6913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6933..6952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6934..6952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6962..6979)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6962..6978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6971..6993)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7120..7575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7207..7226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7417..7438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7601..7705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7706..7774,7775..8566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7924..7943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8737..9325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9226..9245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9479..9496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9613..9634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9649..9654,9655..9672,9673..9679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9687..9703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9692..9714
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9711..9727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9711..9727
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9746..9766
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9748..9766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        9811..10190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcgcgccatg cagggcctga aataacctct gaaagaggaa cttggttagg taccttctga
       61 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtccctt aagaggcgcg ccttaatgta gtcttatgca atactcttgt agtcttgcaa
      301 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
      361 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
      421 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
      481 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      661 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      721 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      781 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      841 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      901 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      961 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1021 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1081 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1141 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1201 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacaattcta
     1981 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2041 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2101 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2161 actttggcgc cggctcgagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
     2221 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     2281 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     2341 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     2401 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     2461 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     2521 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     2581 gactgagtcg gccggtcgaa tcaagcttat cgataccgtc gacggatcca ccggtatgga
     2641 agacgccaaa aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc
     2701 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     2761 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     2821 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     2881 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     2941 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     3001 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     3061 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     3121 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     3181 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac
     3241 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     3301 cagagatcct atttttggca atcaaatcgt tccggatact gcgattttaa gtgttgtccc
     3361 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     3421 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     3481 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     3541 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     3601 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     3661 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     3721 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     3781 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     3841 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     3901 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     3961 taaatacaaa ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc
     4021 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     4081 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     4141 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     4201 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     4261 caagaagggc ggaaagtcca aattgctcga gtgaacgcgt atgcatgagt aactgaggat
     4321 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4381 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4441 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4501 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4561 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4621 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4681 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4741 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4801 gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4861 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca
     4921 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4981 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5041 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5101 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5161 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5221 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5281 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5341 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5401 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5461 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5521 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5581 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga tcgcgttaag
     5641 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5701 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5761 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5821 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5881 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5941 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6001 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6061 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6121 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6181 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     6241 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6301 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6541 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6601 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6721 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6841 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6901 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6961 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     7021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     7081 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     7141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7561 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9721 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9781 gctgcaagcg gtaccgtcga cctgcaggtt gacattgatt attgactagt tattaatagt
     9841 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9901 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9961 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10021 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10081 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10141 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
