LOCUS       sequence-337871-       10508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of murine VP64-Arid3a fusion protein 
            with GFP as reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.VP64-mArid3a.IRES.GFP
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(361..363,364..366,367..1080)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(361..363,364..366,367..1077)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(406..427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(667..686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1014..1035
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1096..1684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1149..1169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1567..1578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1756..1989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2067..2188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2104..2123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2158..2177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2228..2363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2383..2402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2384..2402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2412..2429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2412..2428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2421..2443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2570..3025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2657..2676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2867..2888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3051..3155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3156..3224,3225..4016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3374..3393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4187..4775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4676..4695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4929..4946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5063..5084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5099..5104,5105..5122,5123..5129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5137..5153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5142..5164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5161..5177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5161..5177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5196..5216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5198..5216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        5261..5640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        5697..5888
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5720..5740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5907..6133
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6134..6314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6361..6486
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6979..7212
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7397..7441
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7654..7771
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7829..8235
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8276..8285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8285..8434
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    join(10282..10508,1..348,349..351,352..360)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10449..10466)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
       61 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
      121 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
      181 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
      241 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
      301 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
      361 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      541 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      661 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      721 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      781 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      841 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      901 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      961 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1021 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     1081 tcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1141 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1201 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     1261 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     1321 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     1381 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     1441 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     1501 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     1561 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     1621 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     1681 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     1741 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     1801 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1861 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1921 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1981 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2041 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     2101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2161 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     2221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2341 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     2401 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2461 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     2581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3001 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4981 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5041 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     5101 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     5161 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     5221 aaaagctgga gctgcaagcg gtaccgtcga cctgcaggtt gacattgatt attgactagt
     5281 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     5341 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     5401 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     5461 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     5521 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     5581 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5641 gcgcgccatg cagggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     5701 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5881 atagtccctt aagaggcgcg ccttaatgta gtcttatgca atactcttgt agtcttgcaa
     5941 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6001 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6061 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6121 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6181 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6241 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6301 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6361 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6421 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6481 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6541 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6601 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6661 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6721 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     6781 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     6841 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6901 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6961 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7021 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7081 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7141 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7201 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7261 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7321 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7381 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7441 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7501 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7561 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacaattcta
     7621 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     7681 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     7741 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     7801 actttggcgc cggctcgagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
     7861 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     7921 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     7981 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     8041 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     8101 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     8161 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     8221 gactgagtcg gccggtcgaa tcaagcttat cgataccgtc gacggatcca ccggtgccgc
     8281 catggatgct ttagacgatt ttgacttaga tatgcttggt tcagacgcct tagacgactt
     8341 cgacctagac atgttaggct cagatgcatt ggacgacttc gatttagata tgttgggctc
     8401 cgatgcccta gatgactttg acctagatat gctaggcggt ggaggcagta agttgcaagc
     8461 cgtcatggaa actctcattc agcggcaaca gagagcacga caagaactgg aagctcgcca
     8521 agcccctcct cctccccctc ctgaacctac aggagttcgc gctcgaacaa ccatgactga
     8581 cgaggacaga gagccagaaa atgcccggat gcatcggact cagatggcag ccttggccgc
     8641 catgcgcgct gctgctgctg gattgggtca cccttctagt cccggaggct ctgaagacgg
     8701 gcccccaatc tccggtgatg aagacaccgc cagggagggt actctttcta gtccagcact
     8761 ccatggcagc gtgttggagg gtgctggtca tgccgaggga gatcggcatt tgatggatgt
     8821 tgggtcagac gatgatgata caaagtctaa atgggaagag caggagttgg aagaattggg
     8881 agaggaggaa gaggaagaag aggaggagga cgattttgag gaggaagaag aagaggagga
     8941 gggtttgggt cctcccgaat ccgcctccct ggggacagct ggactcttca cccgaaaggc
     9001 ccctccagca caggcatttc ggggtgacgg agggccccgc atgctctctg gacccgaaag
     9061 attgggtcct ggccccgccc acccaagtca catggcctca cagatgcctc cacccgacca
     9121 tggcgactgg actttcgagg aacaatttaa acagctttat gagctcgacg cagatccaaa
     9181 acggaaagag tttctggatg atctcttctc ctttatgcag aagcgaggca cccctgtaaa
     9241 caggattcca ataatggcta agcaagtact ggacctgttt atgctgtatg tccttgtaac
     9301 tgagaaggga gggctcgtcg aagtaatcaa caagaaactt tggagagaaa ttacaaaggg
     9361 attgaatttg cctaccagta taacctcagc tgcattcaca ctgagaactc agtacatgaa
     9421 atatctctat ccctatgaat gcgaacgcag gggtctttct tctcctaatg aactccaagc
     9481 tgctattgat agtaatagga gagaaggcag gcgccagagc ttcgggggaa gcctcttcgc
     9541 ttatagcccc agtggagccc attcaatgct ccctagtcca aaactccctg tcactccact
     9601 cggactggct gcttctacta atggctcatc aattacccca gcacctaaaa tcaagaagga
     9661 agaagactca gcaatcccaa ttaccgtgcc cggtaggttg cctgtgtcct tggccggcca
     9721 tcctgttgtg gccgcacaag cagcagcagt acaggccgct gccgcacagg ccgctgtcgc
     9781 tgcccaagca gctgcactcg aacaacttcg agaaaaactg gaatccaccg agccacctga
     9841 gaagaaaatg gctctggttg cagatgagca acagagactt atgcagcgcg ctgttcagca
     9901 gtcctttctg gcaatgaccg cccaacttcc catgaacatt agaattaata gccaagcttc
     9961 cgaaagccgc caagatagtg ccgtttcctt gacatctgca aatggttcca atagtatttc
    10021 aatgagtgtt gaaatgaatg ggatagtata tactggcgtc ctttttgccc agcctcctcc
    10081 tcctacagca ccttcagcac caggtaaggg cggagtcagc tctattggta caaataccac
    10141 taccggaagt cgcactgggg ccagtgggtc aaccgtctct ggcgggcagg tgggacttcc
    10201 tggcgtttct actcccacca tgagttccac cagtaacaat tccctgccat aaacgcgtat
    10261 gcatgagtaa ctgaggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
    10321 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
    10381 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
    10441 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
    10501 tctggaag
//
