LOCUS       sequence-337875-       10161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive overexpression of Luciferase cDNA with dTomato as a 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.SFFV.Luciferase.IRES.dTomato
FEATURES             Location/Qualifiers
     source          1..10161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        57..248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        267..493
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             494..674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    721..846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1339..1572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1757..1801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2014..2131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2189..2595
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2636..4285
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2702..2720)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    join(4304..4878,4879..4881,4882..4890)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4471..4488)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4698..4717
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4897..5598
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5030..5048)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5298..5317
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5492..5515
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5617..6205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5670..5690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6088..6099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6277..6510
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6588..6709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6625..6644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6679..6698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6749..6884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6904..6923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6905..6923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6933..6950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6933..6949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7091..7546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7178..7197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7388..7409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7572..7676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7677..7745,7746..8537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7895..7914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8708..9296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9197..9216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9450..9467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9584..9605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9620..9625,9626..9643,9644..9650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9658..9674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9663..9685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9682..9698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9682..9698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9717..9737
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9719..9737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        9782..10161
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcgcgccatg cagggcctga aataacctct gaaagaggaa cttggttagg taccttctga
       61 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtccctt aagaggcgcg ccttaatgta gtcttatgca atactcttgt agtcttgcaa
      301 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
      361 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
      421 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
      481 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      541 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      601 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      661 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
      721 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
      781 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      841 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      901 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      961 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1021 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1081 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1141 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1201 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacaattcta
     1981 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2041 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2101 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2161 actttggcgc cggctcgagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
     2221 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     2281 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     2341 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     2401 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     2461 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     2521 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     2581 gactgagtcg gccggtcgaa tcaagcttat cgataccgtc gacggatcca ccggtatgga
     2641 agacgccaaa aacataaaga aaggcccggc gccattctat cctctagagg atggaaccgc
     2701 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     2761 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg
     2821 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     2881 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     2941 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga acatttcgca
     3001 gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     3061 attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc agggatttca
     3121 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     3181 accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaattcct ctggatctac
     3241 tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat tctcgcatgc
     3301 cagagatcct atttttggca atcaaatcgt tccggatact gcgattttaa gtgttgtccc
     3361 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     3421 cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg attacaaaat
     3481 tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca ctctgattga
     3541 caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga
     3601 agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag gatatgggct
     3661 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     3721 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     3781 gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt ccggttatgt
     3841 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     3901 catagcttac tgggacgaag acgaacactt cttcatagtt gaccgcttga agtctttaat
     3961 taaatacaaa ggatatcagg tggcccccgc tgaattggaa tcgatattgt tacaacaccc
     4021 caacatcttc gacgcgggcg tggcaggtct tcccgacgat gacgccggtg aacttcccgc
     4081 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     4141 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     4201 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     4261 caagaagggc ggaaagtcca aattgctcga gtgaacgcgt tcgcccctct ccctcccccc
     4321 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4381 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4441 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4501 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4561 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4621 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4681 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4741 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     4801 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4861 gaaaaacacg atgataatat ggccacaacc ggatccatgg tgagcaaggg cgaggaggtc
     4921 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     4981 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     5041 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     5101 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     5161 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     5221 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     5281 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     5341 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     5401 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     5461 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     5521 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     5581 atggacgagc tgtacaagtg atcgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     5641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6181 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6241 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6301 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6361 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6421 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6481 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     6541 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6721 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6901 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6961 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7021 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7081 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7141 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7201 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7261 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7321 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7381 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7441 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7501 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7561 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7621 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7681 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7741 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7801 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7861 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7921 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7981 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8041 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8101 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8161 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8221 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8281 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8341 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8401 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8461 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8521 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8581 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8641 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8701 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8761 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8821 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8881 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8941 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9001 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9061 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9661 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9721 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ggtaccgtcg acctgcaggt
     9781 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     9841 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     9901 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     9961 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10021 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10081 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10141 ttagtcatcg ctattaccat g
//
