LOCUS       sequence-337878-       10033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express GFP1-10 in the ER lumen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, 
            Xie J, Kamiyama D
  TITLE     Cell-type-specific, multicolor labeling of endogenous proteins with 
            split fluorescent protein tags in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 
            10.1073/pnas.2024690118.
  PUBMED    34074768
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23). pii: 
            2024690118. doi"; pages: " 10.1073/pnas.2024690118"
COMMENT     Sequence Label: pACUH-GFP1-10 sec
FEATURES             Location/Qualifiers
     source          1..10033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3047..3077,3078..3279)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3360..3454
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3478..3572
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3591..3829
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3776..3797
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3930..4571
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     intron          4679..4744
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4874..4894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5166..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5257..5276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5327..5345)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5797..5868,6270..6543,6618..7272,7334..7649,7853..7984,
                     8055..8669)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9448..10002,10003..10033)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     3001 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac
     3061 ataaggtggt cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt
     3121 gaaaggaata gtattctgag tgtcgtattg agtctgagtg agacagcgat atgattgttg
     3181 attaaccctt agcatgtccg tggggtttga attaactcat aatattaatt agacgaaatt
     3241 atttttaaag ttttattttt aataatttgc gagtacgcaa agcttggctg catccaacgc
     3301 gttgggagct ctccggatcc aagctggccg cggctcgatc cgcttgcatg cctgcaggtc
     3361 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     3421 gtactgtcct ccgagcggag tactgtcctc cgagcggaag cttgcatgcc tgcaggkcgg
     3481 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3541 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata
     3601 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     3661 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     3721 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     3781 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagggaatt
     3841 gggaattcgt taacagatct atgatgtggt gcaaaacagt gatagtgttg ctggcgacag
     3901 tcggctttat tagtgccgag gttagatcta tgtccaaagg agaagaactg tttaccggtg
     3961 ttgtgccaat tttggttgaa ctcgatggtg atgtcaacgg acataagttc tcagtgagag
     4021 gcgaaggaga aggtgacgcc accattggaa aattgactct taaattcatc tgtactactg
     4081 gtaaacttcc tgtaccatgg ccgactctcg taacaacgct tacgtacgga gttcagtgct
     4141 tttcgagata cccagaccat atgaaaagac atgacttttt taagtcggct atgcctgaag
     4201 gttacgtgca agaaagaaca atttcgttca aagatgatgg aaaatataaa actagagcag
     4261 ttgttaaatt tgaaggagat actttggtta accgcattga actgaaagga acagatttta
     4321 aagaagatgg taatattctt ggacacaaac tcgaatacaa ttttaatagt cataacgtat
     4381 acatcactgc tgataagcaa aagaacggaa ttaaagcgaa tttcacagta cgccataatg
     4441 tagaagatgg cagtgttcaa cttgccgacc attaccaaca aaacacccct attggagacg
     4501 gtccggtact tcttcctgat aatcactacc tctcaacaca aacagtcctg agcaaagatc
     4561 caaatgaaaa aggaacaggt ggcggcggaa gttagtctag aggatctttg tgaaggaacc
     4621 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4681 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4741 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4801 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4861 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4921 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4981 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat
     5041 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     5101 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     5161 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5221 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5281 tatcttatca tgtctggatc cactagaagg cctaattcta gtatgtatgt aagttaataa
     5341 aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat
     5401 cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag
     5461 gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct
     5521 ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg
     5581 ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt
     5641 ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa
     5701 ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg
     5761 gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta
     5821 ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt gagtagtaca
     5881 tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca tcaaattgtc
     5941 tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg
     6001 acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt tgagatcata
     6061 tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta caaaaaatat
     6121 aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa gtataaacct
     6181 acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata acgcttgtag
     6241 ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca
     6301 ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc agtccgccgg
     6361 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     6421 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     6481 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     6541 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     6601 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     6661 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     6721 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     6781 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     6841 cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca
     6901 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     6961 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     7021 attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     7081 actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     7141 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     7201 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     7261 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc
     7321 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     7381 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     7441 tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     7501 aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     7561 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     7621 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     7681 acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca
     7741 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat
     7801 ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     7861 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat
     7921 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     7981 aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa
     8041 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     8101 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     8161 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     8221 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     8281 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     8341 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     8401 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     8461 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     8521 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     8581 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     8641 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     8701 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     8761 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     8821 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     8881 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     8941 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     9001 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     9061 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     9121 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     9181 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     9241 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     9301 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     9361 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     9421 gatttcactg gaactagaat taattcggct gctgctctaa acgacgcatt tcgtactcca
     9481 aagtacgaat tttttccctc aagctcttat tttcattaaa caatgaacag gacctaacgc
     9541 acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt
     9601 gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat cactttacta
     9661 ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc aggtgcagcc
     9721 ttccactgcg aatcattaaa gtgggtatca caaatttggg agttttcacc aaggctgcac
     9781 ccaaggctct gctcccacaa ttttctctta atagcacact tcggcacgtg aattaatttt
     9841 actccagtca cagctttgca gcaaaatttg caatatttca ttttttttta ttccacgtaa
     9901 gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca acaagcaaac
     9961 gtgcactgaa tttaagtgta tacttcggta agcttcggct atcgacggga ccaccttatg
    10021 ttatttcatc atg
//
