LOCUS       sequence-337879-       12288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of human ARID3A cDNA with dTomato 
            as reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.T...
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5166..5740,5741..5743,5744..5752)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5333..5350)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5560..5579
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5759..6460
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5892..5910)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6160..6179
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6354..6377
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6485..6984
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6520..6543)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6917..6936
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6991..7000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6997..6999,7000..7002,7003..7713)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7726..7782
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7783..8529
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8556..9144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8609..8629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9027..9038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9216..9449
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9527..9648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9564..9583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9618..9637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9688..9823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9843..9862)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9844..9862)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9872..9889)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9872..9888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9881..9903)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10030..10485
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10117..10136)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10327..10348
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10511..10615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10616..10684,10685..11476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10834..10853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11647..12235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12136..12155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
     3061 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag
     3121 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagac cggtatgaag ttgcaggcag tgatggagac cctattgcag cgacaacagc
     3421 gggcgagaca ggagctcgaa gcgaggcaac aattgccacc cgatcctccc gcagctccgc
     3481 ccggcagagc tagggccgca cctgacgaag accgagagcc agagtcagca agaatgcaac
     3541 gggcacagat ggccgctctt gccgctatgc gcgcagcagc cgcgggactt ggtcacccgg
     3601 catcacctgg agggtcagag gacgggcccc caggttcaga ggaggaagat gcggcccgag
     3661 aggggacccc gggatctcct ggccggggcc gagaaggccc cggagaagag catttcgagg
     3721 acatggcaag cgatgaagat atgaaaccta agtgggaaga ggaagaaatg gaggaggacc
     3781 tcggcgaaga cgaggaagaa gaagaagagg actacgagga tgaagaggaa gaagaggatg
     3841 aagagggact gggtccgcct ggccctgctt cccttgggac aaccgcgctg ttcccgcgaa
     3901 aggctcagcc accgcaggca tttaggggag acggggtgcc acgggtgctt ggaggacagg
     3961 aacgccccgg acccgggcct gcccatccgg gaggggcggc tcacgtggcc ccacagttgc
     4021 agcctccgga ccacggggat tggacgtatg aagaacaatt taagcagctg tatgagctgg
     4081 atggcgaccc gaaacggaaa gaattcctcg acgacctttt ttcattcatg cagaagcggg
     4141 gtactcctgt taatcgcata cccatcatgg cgaagcaggt ccttgacctc tttatgcttt
     4201 atgtgcttgt tactgaaaaa ggtggcctgg tcgaggttat aaacaagaag ctctggaggg
     4261 aaattacaaa gggacttaat ttgcccactt caattacgag cgcggcgttt acgctgagaa
     4321 cgcaatacat gaaatatttg tacccctatg aatgtgagaa aagaggactt tcaaacccga
     4381 atgagcttca ggctgccatc gacagcaacc ggagggaggg caggagacag tctttcgggg
     4441 gctcactctt tgcatatagc ccgggtggag cccatgggat gctctcaagt ccgaaattgc
     4501 ctgtcagcag tctggggctc gccgctagca cgaacggatc atcaatcact cccgcaccta
     4561 agatcaagaa agaggaagat tccgcgattc ccattacggt tcccggtcgg ttgccagtgt
     4621 cacttgctgg ccatccagtc gtagctgccc aggcagccgc cgttcaggcg gctgcagctc
     4681 aagccgctgt agcggctcag gcagcagcgt tggagcaatt gcgagagaaa ctggagtctg
     4741 ccgaaccgcc agagaagaaa atggcccttg tagccgatga gcagcaacgc ttgatgcaga
     4801 gggcgctcca acagaacttc ctcgccatgg ctgcgcagct gcctatgtcc attcggatta
     4861 attcacaagc aagtgaaagc cgccaggact ctgctgtgaa tctcacagga accaacggat
     4921 ccaactctat atcaatgtca gtagaaatca acggtatcat gtacactggc gttctttttg
     4981 cgcagccccc ggcgcccact cctacgagcg cacccaacaa aggaggtggc ggtggcgggg
     5041 gttccagtag caatgcaggc gggcgcggtg gcaataccgg aacaagtggc gggcaagcag
     5101 gacctgccgg gttgagcact cccagcactt ctacatctaa caactcattg ccgtaaacgc
     5161 gttcgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5221 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5281 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5341 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5401 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5461 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5521 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5581 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5641 cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     5701 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa ccggatccat
     5761 ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     5821 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5881 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     5941 cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     6001 cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6061 ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     6121 caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     6181 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     6241 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     6301 ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga
     6361 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     6421 ccaccacctg ttcctgtacg gcatggacga gctgtacaag tgagcgatat cgcgtaattc
     6481 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     6541 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccgga
     6601 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     6661 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     6721 gcacgtgtca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     6781 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6841 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6901 tccaccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6961 tcttcctcat ctccgggcct ttcggctagc gccaccatgg gatctaaggg cgaggaactg
     7021 ttcaccggcg tggtgcccat cctggtggaa ctggatggcg acgtgaacgg ccacaagttc
     7081 tctgtgcggg gagagggcga aggcgacgcc acaaatggca agctgaccct gaagttcatc
     7141 tgcaccaccg gcaagctgcc cgtgccttgg cctaccctcg tgaccacact gacctacggc
     7201 gtgcagtgct tcagcagata ccccgaccat atgaagcggc acgacttctt caagagcgcc
     7261 atgcccgagg gctacgtgca ggaacggacc atcagcttca aggacgacgg cacctacaag
     7321 accagagccg aagtgaagtt cgagggcgac accctcgtga accggatcga gctgaagggc
     7381 atcgatttca aagaggacgg caacatcctg ggccacaagc tggagtacaa cttcaacagc
     7441 cacaatgtgt atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     7501 cggcacaacg tggaagatgg cagcgtgcag ctggccgacc actaccagca gaacaccccc
     7561 atcggagatg gccccgtgct gctgcccgac aaccactacc tgagcaccca gagcgtgctg
     7621 agcaaggacc ccaacgagaa gcgggaccac atggtgctgc tggaattcgt gaccgccgct
     7681 ggcatcaccc acggcatgga cgagctgtat aagggaggaa gcggcgccac caatttcagc
     7741 ctgctgaaac aggccggcga cgtggaagag aaccctggcc ctatgagccg gctggacaag
     7801 agcaaagtga tcaacagcgc cctggaactg ctgaacggcg tgggcatcga gggcctgacc
     7861 acccggaagc tggcccagaa actgggcgtg gaacagccca ccctgtactg gcacgtgaag
     7921 aacaagcggg ccctgctgga cgccctgccc atcgagatgc tggaccggca ccacacccac
     7981 agctgccctc tggaaggcga gagctggcag gacttcctgc ggaacaacgc caagagctac
     8041 agatgcgccc tgctgagcca ccgggacggc gccaaagtgc acctgggcac cagacccacc
     8101 gagaagcagt acgagacact ggaaaaccag ctggccttcc tgtgccagca gggcttcagc
     8161 ctggaaaacg ccctgtacgc cctgagcgcc gtgggccact tcaccctggg ctgcgtgctg
     8221 gaagaacagg aacaccaggt cgccaaagag gaaagagaga cacccaccac cgacagcatg
     8281 ccccccctgc tgaagcaggc catcgagctg ttcgacagac agggcgccga gcccgccttc
     8341 ctgttcggcc tggaactgat catctgcggc ctcgagaagc agctgaagtg cgagagcggc
     8401 ggacccaccg acgccctgga cgacttcgac ctggacatgc tgcccgccga tgccctggat
     8461 gattttgatc tggatatgct ccctgctgac gctctcgatg acttcgatct cgatatgctg
     8521 ccaggctaat gtacaagtga tcgcgttaag tcgacaatca acctctggat tacaaaattt
     8581 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8641 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8701 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8761 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8821 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     8881 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     8941 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     9001 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9061 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     9121 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     9181 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9241 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9421 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     9481 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9541 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9601 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9661 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     9841 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     9901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    10021 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    10141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    10201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    10261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    10321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    10381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    10441 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    10501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    11101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    11161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    11221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    11281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    11341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    12001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    12061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    12121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    12181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    12241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgc
//
