LOCUS       sequence-337880-       10094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express GFP1-10 in the mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, 
            Xie J, Kamiyama D
  TITLE     Cell-type-specific, multicolor labeling of endogenous proteins with 
            split fluorescent protein tags in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 
            10.1073/pnas.2024690118.
  PUBMED    34074768
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23). pii: 
            2024690118. doi"; pages: " 10.1073/pnas.2024690118"
COMMENT     Sequence Label: pACUH-GFP1-10 mitochondrial matrix
FEATURES             Location/Qualifiers
     source          1..10094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3047..3077,3078..3279)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3360..3454
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3478..3572
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3591..3829
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3776..3797
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3852..3926
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3945..4586
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     CDS             4602..4631
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          4740..4805
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4935..4955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5227..5361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5264..5283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5318..5337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5388..5406)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5858..5929,6331..6604,6679..7333,7395..7710,7914..8045,
                     8116..8730)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9509..10063,10064..10094)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     3001 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagaccatg atgaaataac
     3061 ataaggtggt cccgtcggca agagacatcc acttaacgta tgcttgcaat aagtgcgagt
     3121 gaaaggaata gtattctgag tgtcgtattg agtctgagtg agacagcgat atgattgttg
     3181 attaaccctt agcatgtccg tggggtttga attaactcat aatattaatt agacgaaatt
     3241 atttttaaag ttttattttt aataatttgc gagtacgcaa agcttggctg catccaacgc
     3301 gttgggagct ctccggatcc aagctggccg cggctcgatc cgcttgcatg cctgcaggtc
     3361 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     3421 gtactgtcct ccgagcggag tactgtcctc cgagcggaag cttgcatgcc tgcaggtcgg
     3481 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3541 actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagcg ccggagtata
     3601 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     3661 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     3721 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     3781 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagggaatt
     3841 gggaattcac catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc
     3901 ggcggctccc agtgccgcgc gccaagatcc attcgttgac tagtatgtcc aaaggagaag
     3961 aactgtttac cggtgttgtg ccaattttgg ttgaactcga tggtgatgtc aacggacata
     4021 agttctcagt gagaggcgaa ggagaaggtg acgccaccat tggaaaattg actcttaaat
     4081 tcatctgtac tactggtaaa cttcctgtac catggccgac tctcgtaaca acgcttacgt
     4141 acggagttca gtgcttttcg agatacccag accatatgaa aagacatgac ttttttaagt
     4201 cggctatgcc tgaaggttac gtgcaagaaa gaacaatttc gttcaaagat gatggaaaat
     4261 ataaaactag agcagttgtt aaatttgaag gagatacttt ggttaaccgc attgaactga
     4321 aaggaacaga ttttaaagaa gatggtaata ttcttggaca caaactcgaa tacaatttta
     4381 atagtcataa cgtatacatc actgctgata agcaaaagaa cggaattaaa gcgaatttca
     4441 cagtacgcca taatgtagaa gatggcagtg ttcaacttgc cgaccattac caacaaaaca
     4501 cccctattgg agacggtccg gtacttcttc ctgataatca ctacctctca acacaaacag
     4561 tcctgagcaa agatccaaat gaaaaaggtg gcggaggaag tgaacaaaaa ctcatctcag
     4621 aagaggatct gtaagcggcc gcggctcgag ggtacctcta gaggatcttt gtgaaggaac
     4681 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     4741 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     4801 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     4861 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     4921 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     4981 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     5041 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     5101 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     5161 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5221 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5281 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5341 gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg taagttaata
     5401 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     5461 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     5521 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     5581 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     5641 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     5701 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     5761 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     5821 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     5881 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     5941 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     6001 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     6061 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     6121 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     6181 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     6241 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     6301 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     6361 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     6421 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     6481 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     6541 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     6601 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     6661 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     6721 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     6781 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     6841 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     6901 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     6961 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     7021 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     7081 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     7141 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     7201 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     7261 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     7321 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     7381 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     7441 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     7501 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     7561 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     7621 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     7681 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     7741 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     7801 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     7861 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     7921 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     7981 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     8041 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     8101 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     8161 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     8221 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     8281 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
     8341 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
     8401 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
     8461 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     8521 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     8581 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
     8641 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     8701 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     8761 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     8821 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     8881 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
     8941 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     9001 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     9061 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     9121 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
     9181 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
     9241 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     9301 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     9361 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     9421 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     9481 ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat ttcgtactcc
     9541 aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca ggacctaacg
     9601 cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt actctgtttg
     9661 tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta tcactttact
     9721 aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc
     9781 cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac caaggctgca
     9841 cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt gaattaattt
     9901 tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt attccacgta
     9961 agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa
    10021 cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg accaccttat
    10081 gttatttcat catg
//
