LOCUS       sequence-33789-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pCold TDP43 WT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..29
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             30..41
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             54..707
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     665..687
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             723..743
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atcacaaagt gcatcatcat catcatcata tcgaaggtag gcatatggag ctcatgtccc
       61 ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt cttttggaat
      121 atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat aaatggcgaa
      181 acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt gatggtgatg
      241 ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac aacatgttgg
      301 gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt ttggatatta
      361 gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa gttgattttc
      421 ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat aaaacatatt
      481 taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt gatgttgttt
      541 tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt aaaaaacgta
      601 ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata gcatggcctt
      661 tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg gatctggcta
      721 gcgagaatct ttattttcag ggatccatgt ctgaatatat tcgggtaacc gaagatgaga
      781 acgatgagcc cattgaaata ccatcggaag acgatgggac ggtgctgctc tccacggtta
      841 cagcccagtt tccaggggcg tgtgggcttc gctacaggaa
//
