LOCUS       sequence-337890-       10483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 
            related (MERS) pseudovirus assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Rogers TF, Zhao F, Huang D, Beutler N, Burns A, He WT, Limbo O, 
            Smith C, Song G, Woehl J, Yang L, Abbott RK, Callaghan S, Garcia E, 
            Hurtado J, Parren M, Peng L, Ramirez S, Ricketts J, Ricciardi MJ, 
            Rawlings SA, Wu NC, Yuan M, Smith DM, Nemazee D, Teijaro JR, Voss 
            JE, Wilson IA, Andrabi R, Briney B, Landais E, Sok D, Jardine JG, 
            Burton DR
  TITLE     Isolation of potent SARS-CoV-2 neutralizing antibodies and 
            protection from disease in a small animal model.
  JOURNAL   Science. 2020 Aug 21;369(6506):956-963. doi: 
            10.1126/science.abc7520. Epub 2020 Jun 15.
  PUBMED    32540903
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc7520"; journalName: "Science"; date: 
            "2020-08-21- 21"; volume: "369"; issue: "6506"; pages: "956-963"
COMMENT     Sequence Label: pBOBI-FLuc
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2718..4370
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2784..2802)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4457..4686
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4669..4688
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4863..5451
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4916..4936)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5334..5345)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5858..6038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6064..6081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6070..6294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6340..6768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6427..6446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6637..6658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6777..6797)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6782..7111
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6962..7097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7024..7043
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7159..7206
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7225..7599
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7729..7850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7766..7785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7820..7839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7899..7915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7899..7915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7912..7934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7923..7939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7947..7953,7954..7971,7972..7977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7992..8013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8130..8147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8301..8889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8381..8400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9060..9851,9852..9920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9683..9702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9921..10025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10100..10119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10291..10483,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaact
     2701 ctagagaatt cggatccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct
     2761 atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc
     2821 tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg
     2881 agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa
     2941 atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg
     3001 cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc
     3061 tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa
     3121 aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta
     3181 aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg
     3241 gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga
     3301 tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg
     3361 cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata
     3421 ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat
     3481 atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga
     3541 ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct
     3601 tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg
     3661 gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag
     3721 gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg
     3781 gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg
     3841 atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc
     3901 ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg
     3961 atggatggct acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg
     4021 ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg
     4081 aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg
     4141 atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg
     4201 aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag
     4261 gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa
     4321 tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ctcgagccat
     4381 ggaagcttga tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg
     4441 agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga
     4501 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
     4561 agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt
     4621 ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt gcctttctct
     4681 ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt
     4741 ccgccgatcc tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4801 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4861 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4921 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4981 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5041 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5101 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5161 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5221 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5281 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5341 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5401 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5461 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5521 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     5581 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     5641 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     5701 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     5761 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     5821 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat
     6061 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6121 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6181 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6241 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6301 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     6781 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6841 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6901 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6961 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7021 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7081 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7141 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     7201 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     7261 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     7321 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7381 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7441 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7501 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7561 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     7621 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7681 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7861 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10021 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    10081 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    10141 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    10201 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    10261 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
    10321 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    10381 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10441 cgtatgttcc catagtaacg ccaataggga ctttccattg acg
//
