LOCUS       sequence-33791-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pCold TDP43 G294A
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..23
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             42..53
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtatcaccat gaatcacaaa gtgcatcatc atcatcatca tatcgaaggt aggcatatgg
       61 agctcggtac cctcgaggga tccatgtctg aatatattcg ggtaaccgaa gatgagaacg
      121 atgagcccat tgaaatacca tcggaagacg atgggacggt gctgctctcc acggttacag
      181 cccagtttcc aggggcgtgt gggcttcgct acaggaatcc agtgtctcag tgtatgagag
      241 gtgtccggct ggtagaagga attctgcatg ccccagatgc tggctgggga aatctggtgt
      301 atgttgtcaa ctatccaaaa gataacaaaa gaaaaatgga tgagacagat gcttcatcag
      361 cagtgaaagt gaaaagagca gtccagaaaa catccgattt aatagtgttg ggtctcccat
      421 ggaaaacaac cgaacaggac ctgaaagagt attttagtac ctttggagaa gttcttatgg
      481 tgcaggtcaa gaaagatctt aagactggtc attcaaaggg gtttggcttt gttcgtttta
      541 cggaatatga aacacaagtg aaagtaatgt cacagcgaca tatgatagat ggacgatggt
      601 gtgactgcaa acttcctaat tctaagcaaa gccaagatga gcctttgaga agcagaaaag
      661 tgtttgtggg gcgctgtaca gaggacatga ctgaggatga gctgcgggag ttcttctctc
      721 agtacgggga tgtgatggat gtcttcatcc ccaagccatt cagggccttt gcctttgtta
      781 catttgcaga tgatcagatt gcgcagtctc tttgtggaga ggacttgatc attaaaggaa
      841 tca
//
