LOCUS       sequence-337915-        3355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries CBM9 fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie 
            RL, Winkler DFH, Pelech S, Nano FE
  TITLE     Escherichia coli recombinant expression of SARS-CoV-2 protein 
            fragments.
  JOURNAL   Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 
            10.1186/s12934-022-01753-0.
  PUBMED    35123472
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-02-5"; volume: "21(1):21. doi"; pages: " 
            10.1186/s12934-022-01753-0"
COMMENT     Sequence Label: CBM9-(PT4)P
FEATURES             Location/Qualifiers
     source          1..3355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     488..507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        488..506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             538..560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1224..1242)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1238..1285
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1382..1837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1469..1488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1679..1700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1863..1967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1968..2036,2037..2828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2186..2205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2999..3355,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tctcgatccc
      481 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt
      541 gtttaacttt aagaaggaga tatacatatg gctagcatga ctggtggtag cggtatcatg
      601 gttgctaccg ctaaatacgg taccccggtt atcgacggtg aaatcgacga aatctggaac
      661 accaccgaag aaatcgaaac caaagctgtt gctatgggtt ctctggacaa aaacgctacc
      721 gctaaagttc gtgttctgtg ggacgaaaac tacctgtacg ttctggctat cgttaaagac
      781 ccggttctga acaaagacaa ctctaacccg tgggaacagg actctgttga aatcttcatc
      841 gacgaaaaca accacaaaac cggttactac gaagacgacg acgctcagtt ccgtgttaac
      901 tacatgaacg aacagacctt cggtaccggt ggttctccgg ctcgtttcaa aaccgctgtt
      961 aaactgatcg aaggtggtta catcgttgaa gctgctatca aatggaaaac catcaaaccg
     1021 accccgaaca ccgttatcgg tttcaacatc caggttaacg acgctaacga aaaaggtcag
     1081 cgtgttggta tcatctcttg gtctgacccg accaacaact cttggcgtga cccgtctaaa
     1141 ttcggtaacc tgcgtctgat caaaccgacg ccaacaccta ccccaacacc gtaatgaaat
     1201 gcgatgtacg tgctatgctc cttccgctga gcaataacta gcataacccc ttggggcctc
     1261 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat ctggcgtaat
     1321 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1381 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     1681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1801 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     1861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaac
//
