LOCUS       sequence-337916-        3771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Spike epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie 
            RL, Winkler DFH, Pelech S, Nano FE
  TITLE     Escherichia coli recombinant expression of SARS-CoV-2 protein 
            fragments.
  JOURNAL   Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 
            10.1186/s12934-022-01753-0.
  PUBMED    35123472
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-02-5"; volume: "21(1):21. doi"; pages: " 
            10.1186/s12934-022-01753-0"
COMMENT     Sequence Label: CBM9-ID-H1
FEATURES             Location/Qualifiers
     source          1..3771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     506..525
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        506..524
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             556..578
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1658..1676)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1672..1719
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1816..2271
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1903..1922)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2113..2134
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2297..2401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2402..2470,2471..3262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRRQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2620..2639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3433..3771,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcaggatc tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt
      541 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagcatgact
      601 ggtggtagcg gtatcatggt tgctaccgct aaatacggta ccccggttat cgacggtgaa
      661 atcgacgaaa tctggaacac caccgaagaa atcgaaacca aagctgttgc tatgggttct
      721 ctggacaaaa acgctaccgc taaagttcgt gttctgtggg acgaaaacta cctgtacgtt
      781 ctggctatcg ttaaagaccc ggttctgaac aaagacaact ctaacccgtg ggaacaggac
      841 tctgttgaaa tcttcatcga cgaaaacaac cacaaaaccg gttactacga agacgacgac
      901 gctcagttcc gtgttaacta catgaacgaa cagaccttcg gtaccggtgg ttctccggct
      961 cgtttcaaaa ccgctgttaa actgatcgaa ggtggttaca tcgttgaagc tgctatcaaa
     1021 tggaaaacca tcaaaccgac cccgaacacc gttatcggtt tcaacatcca ggttaacgac
     1081 gctaacgaaa aaggtcagcg tgttggtatc atctcttggt ctgacccgac caacaactct
     1141 tggcgtgacc cgtctaaatt cggtaacctg cgtctgatca aaccgacgcc aacacctacc
     1201 ccaacaccga acttcaactt caacggtctg accggtaccg gtgttctgac cgaatctaac
     1261 aaaaaattcc tgccgttcca gcagttcggt cgtgacatcg ctgacaccac cgacgctgtt
     1321 cgtgacccgc agaccctgga aatcctggac atcacctaat gacttgcgat gtacgtgcta
     1381 tgctcctttg agacgaatac ctgtaaatcg atttcaatta cgtctcaagg agcgatttac
     1441 atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agaaggtttc tagagtccat
     1501 ataggatagg agattgatgt atgatgagga aaaagagttt ttggcttggg atgctaactg
     1561 ccttcatgct cgtgttcacg atggcattca gcgattccgc ttctgctgct caaccgtgag
     1621 acgttaaggc taggtggagg ctcattcgtc tcaccttccg ctgagcaata actagcataa
     1681 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     1741 ggatctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     1801 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1861 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1921 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1981 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2041 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2101 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2161 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2221 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     2281 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3001 ttcccggcga caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3301 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3361 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac a
//
