LOCUS       sequence-337917-        3409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Nucleocapsid protein epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie 
            RL, Winkler DFH, Pelech S, Nano FE
  TITLE     Escherichia coli recombinant expression of SARS-CoV-2 protein 
            fragments.
  JOURNAL   Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 
            10.1186/s12934-022-01753-0.
  PUBMED    35123472
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-02-5"; volume: "21(1):21. doi"; pages: " 
            10.1186/s12934-022-01753-0"
COMMENT     Sequence Label: CBM9-N
FEATURES             Location/Qualifiers
     source          1..3409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     488..507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        488..506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             538..560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1278..1296)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1292..1339
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1436..1891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1523..1542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1733..1754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1917..2021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2022..2090,2091..2882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2240..2259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3053..3409,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tctcgatccc
      481 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt
      541 gtttaacttt aagaaggaga tatacatatg gctagcatga ctggtggtag cggtatcatg
      601 gttgctaccg ctaaatacgg taccccggtt atcgacggtg aaatcgacga aatctggaac
      661 accaccgaag aaatcgaaac caaagctgtt gctatgggtt ctctggacaa aaacgctacc
      721 gctaaagttc gtgttctgtg ggacgaaaac tacctgtacg ttctggctat cgttaaagac
      781 ccggttctga acaaagacaa ctctaacccg tgggaacagg actctgttga aatcttcatc
      841 gacgaaaaca accacaaaac cggttactac gaagacgacg acgctcagtt ccgtgttaac
      901 tacatgaacg aacagacctt cggtaccggt ggttctccgg ctcgtttcaa aaccgctgtt
      961 aaactgatcg aaggtggtta catcgttgaa gctgctatca aatggaaaac catcaaaccg
     1021 accccgaaca ccgttatcgg tttcaacatc caggttaacg acgctaacga aaaaggtcag
     1081 cgtgttggta tcatctcttg gtctgacccg accaacaact cttggcgtga cccgtctaaa
     1141 ttcggtaacc tgcgtctgat caaaccgacg ccaacaccta ccccaacacc gcagaaaaaa
     1201 cagcagaccg ttaccctgct gccggctgct gacctggacg acttctaatg acttgcgatg
     1261 tacgtgctat gctccttccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     1321 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatctggcg taatagcgaa
     1381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     1441 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1501 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1561 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1621 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1681 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1741 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1801 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1861 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1921 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1981 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2041 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2101 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2161 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2221 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2281 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2341 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2401 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2461 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2521 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2581 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2641 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2701 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2761 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2821 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2881 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2941 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3001 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3061 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3121 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3181 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3241 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3301 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaac
//
