LOCUS       sequence-337918-        3435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 Spike epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   McGuire BE, Mela JE, Thompson VC, Cucksey LR, Stevens CE, McWhinnie 
            RL, Winkler DFH, Pelech S, Nano FE
  TITLE     Escherichia coli recombinant expression of SARS-CoV-2 protein 
            fragments.
  JOURNAL   Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 
            10.1186/s12934-022-01753-0.
  PUBMED    35123472
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-02-5"; volume: "21(1):21. doi"; pages: " 
            10.1186/s12934-022-01753-0"
COMMENT     Sequence Label: CBM9-ID-F
FEATURES             Location/Qualifiers
     source          1..3435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     488..507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        488..506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             538..560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1304..1322)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1318..1365
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1462..1917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1549..1568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1759..1780
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1943..2047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2048..2116,2117..2908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2266..2285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3079..3435,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tctcgatccc
      481 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag aaataatttt
      541 gtttaacttt aagaaggaga tatacatatg gctagcatga ctggtggtag cggtatcatg
      601 gttgctaccg ctaaatacgg taccccggtt atcgacggtg aaatcgacga aatctggaac
      661 accaccgaag aaatcgaaac caaagctgtt gctatgggtt ctctggacaa aaacgctacc
      721 gctaaagttc gtgttctgtg ggacgaaaac tacctgtacg ttctggctat cgttaaagac
      781 ccggttctga acaaagacaa ctctaacccg tgggaacagg actctgttga aatcttcatc
      841 gacgaaaaca accacaaaac cggttactac gaagacgacg acgctcagtt ccgtgttaac
      901 tacatgaacg aacagacctt cggtaccggt ggttctccgg ctcgtttcaa aaccgctgtt
      961 aaactgatcg aaggtggtta catcgttgaa gctgctatca aatggaaaac catcaaaccg
     1021 accccgaaca ccgttatcgg tttcaacatc caggttaacg acgctaacga aaaaggtcag
     1081 cgtgttggta tcatctcttg gtctgacccg accaacaact cttggcgtga cccgtctaaa
     1141 ttcggtaacc tgcgtctgat caaaccgacg ccaacaccta ccccaacacc ggactctaaa
     1201 gttggtggta actacaacta cctgtaccgt ctgttccgta aatctaacct gaaaccgttc
     1261 gaacgtgaca tctctaccga aatctaccag taatgaagcc cttccgctga gcaataacta
     1321 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     1381 atatccggat ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1441 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1501 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1561 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1621 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1681 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1741 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1801 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1861 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     1921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3421 cagcttggag cgaac
//
