LOCUS       sequence-337923-        3492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35E3_STOP
FEATURES             Location/Qualifiers
     source          1..3492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1049..1148)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1165..1184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1166..1184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1189..1205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1189..1205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1307..1326)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1318..2127
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1385..1404)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2274..2862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2763..2782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3016..3033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3192..3219)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3311..3397)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3446..3468
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3460..3477
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3461..3477
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc actgctggtg
      121 gaccgggtga gaggacactg gaggatcgca gcaggactgc tgtttaacct gctggtgtcc
      181 atctgcatcg tgttcctgaa taagtggatc tacgtgtatc acggctttcc taacatgtct
      241 ctgaccctgg tgcacttcgt ggtgacatgg ctgggcctgt acatctgcca gaagctggat
      301 atctttgcac caaagagcct gccaccttcc aggctgctgc tgctggccct gtccttctgt
      361 ggctttgtgg tgttcaccaa tctgtctctg cagaacaata ccatcggcac ataccagctg
      421 gccaaggcca tgaccacacc tgtgatcatc gccatccaga cattttgtta tcagaagacc
      481 ttctctacaa ggatccagct gaccctgatc ccaatcacac tgggcgtgat cctgaacagc
      541 tactatgacg tgaagttcaa ttttctggga atggtgttcg ccgccctggg cgtgctggtg
      601 accagcctgt atcaagtgtg ggtgggagca aagcagcacg agctgcaggt gaactccatg
      661 cagctgctgt actatcaggc ccccatgagc tccgccatgc tgctggtggc agtgccattc
      721 tttgagcccg tgtttggcga gggcggcatc ttcggccctt ggtctgtgag cgccctgctg
      781 atggtgctgc tgagcggcgt gatcgccttt atggtgaatc tgtctatcta ctggatcatc
      841 ggcaacacca gcccagtgac atataatatg ttcggccact tcaagttttg catcaccctg
      901 tttggcggct acgtgctgtt caaggatccc ctgagcatca accaggccct gggcatcctg
      961 tgcaccctgt ttggcatcct ggcctataca cacttcaagc tgtccgagca ggagggctcc
     1021 aggtctaagc tggcccagcg cccctaagac ccagctttct tgtacaaagt tggcattata
     1081 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1141 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1201 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1261 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1321 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1381 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1441 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1501 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1561 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1621 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1681 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1741 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1801 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1861 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     1921 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     1981 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2041 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2101 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2161 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2221 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2401 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2941 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3001 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3061 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3121 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3181 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3241 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3301 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3361 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3421 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3481 aagctcgggc cc
//
