LOCUS       sequence-337925-        4500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC28A1_STOP
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2057..2156)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2173..2192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2174..2192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2197..2213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2197..2213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2315..2334)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2326..3135
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2393..2412)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3282..3870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3771..3790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4024..4041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4200..4227)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4319..4405)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4454..4476
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4468..4485
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4469..4485
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gaacgaccct
      121 agccggagaa gggagtccat ctctctgacc ccagtggcca agggcctgga gaatatgggc
      181 gccgacttcc tggagtccct ggaggaggga cagctgccaa gaagcgatct gtcccccgcc
      241 gagatcagga gctcctggtc tgaggcagca ccaaagcctt ttagcagatg gaggaatctg
      301 cagcctgccc tgagggcacg gtccttctgc cgcgagcaca tgcagctgtt tcggtggatc
      361 ggaaccggac tgctgtgcac aggactgtct gccttcctgc tggtggcctg tctgctggac
      421 ttccagagag ccctggccct gtttgtgctg acctgcgtgg tgctgacctt cctgggccac
      481 agactgctga agaggctgct gggaccaaag ctgcgccggt ttctgaagcc acagggccac
      541 cccagactgc tgctgtggtt caagagggga ctggccctgg cagcctttct gggactggtg
      601 ctgtggctgt ccctggatac ctctcagaga cctgagcagc tggtgtcttt tgccggcatc
      661 tgcgtgttcg tggccctgct gtttgcctgc agcaagcacc actgtgccgt gtcttggagg
      721 gccgtgagct ggggactggg actgcagttc gtgctgggac tgctggtcat cagaaccgag
      781 ccaggcttca tcgcctttga gtggctgggc gagcagatca ggatctttct gagctacaca
      841 aaggccggct ctagcttcgt gtttggcgag gccctggtga aggacgtgtt cgcctttcag
      901 gtgctgccca tcatcgtgtt ctttagctgc gtgatctccg tgctgtatca cgtgggcctg
      961 atgcagtggg tcatcctgaa gatcgcctgg ctgatgcagg tgaccatggg caccacagcc
     1021 accgagacac tgtccgtggc cggcaacatc ttcgtgtctc agaccgaggc accactgctg
     1081 atcaggcctt acctggccga catgacactg tccgaggtgc acgtggtcat gaccggagga
     1141 tacgcaacaa tcgcaggaag cctgctggga gcctatatct ccttcggcat cgatgccaca
     1201 tccctgatcg cagcatctgt gatggcagca ccatgcgccc tggccctgag caagctggtg
     1261 taccctgagg tggaggagtc caagtttaga agggaggagg gcgtgaagct gacctatggc
     1321 gacgcccaga atctgatcga ggcagcaagc acaggagcag caatctccgt gaaggtggtg
     1381 gcaaacatcg ccgccaatct gatcgccttc ctggccgtgc tggacttcat caacgccgcc
     1441 ctgagctggc tgggcgacat ggtggatatc cagggcctgt ctttccagct gatctgcagc
     1501 tatatcctgc gcccagtggc ctttctgatg ggagtggcat gggaggactg tccagtggtg
     1561 gcagagctgc tgggcatcaa gctgttcctg aatgagtttg tggcctacca ggacctgagc
     1621 aagtataagc agcgccggct ggcaggagca gaggagtggg tgggcgatcg caagcagtgg
     1681 atctccgtgc gggccgaggt gctgaccaca ttcgccctgt gcggcttcgc caacttttcc
     1741 tctatcggca tcatgctggg aggactgacc agcatggtgc ctcagcgcaa gtctgacttc
     1801 agccagatcg tgctgcgggc cctgtttaca ggcgcctgcg tgtctctggt gaacgcctgt
     1861 atggcaggaa tcctgtacat gccaagggga gccgaggtgg attgcatgtc cctgctgaat
     1921 accacactga gctcctctag cttcgagatc tatcagtgct gtcgcgaggc cttccagtcc
     1981 gtgaaccctg agttttctcc agaggccctg gataactgct gtcggtttta caatcacacc
     2041 atctgtgccc agtaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     2101 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     2161 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     2221 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2281 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2341 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2401 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2461 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2521 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2581 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2641 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2701 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2761 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2821 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2881 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2941 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     3001 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     3061 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     3121 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     3181 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4081 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4141 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4201 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4261 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4321 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4381 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4441 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
