LOCUS       sequence-33793-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pCold TDP43 M337V
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             27..38
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccaagtgca tcatcatcat catcatatcg aaggtaggca tatggagctc ggtaccctcg
       61 agggatccat gtctgaatat attcgggtaa ccgaagatga gaacgatgag cccattgaaa
      121 taccatcgga agacgatggg acggtgctgc tctccacggt tacagcccag tttccagggg
      181 cgtgtgggct tcgctacagg aatccagtgt ctcagtgtat gagaggtgtc cggctggtag
      241 aaggaattct gcatgcccca gatgctggct ggggaaatct ggtgtatgtt gtcaactatc
      301 caaaagataa caaaagaaaa atggatgaga cagatgcttc atcagcagtg aaagtgaaaa
      361 gagcagtcca gaaaacatcc gatttaatag tgttgggtct cccatggaaa acaaccgaac
      421 aggacctgaa agagtatttt agtacctttg gagaagttct tatggtgcag gtcaagaaag
      481 atcttaagac tggtcattca aaggggtttg gctttgttcg ttttacggaa tatgaaacac
      541 aagtgaaagt aatgtcacag cgacatatga tagatggacg atggtgtgac tgcaaacttc
      601 ctaattctaa gcaaagccaa gatgagcctt tgagaagcag aaaagtgttt gtggggcgct
      661 gtacagagga catgactgag gatgagctgc gggagttctt ctctcagtac ggggatgtga
      721 tggatgtctt catccccaag ccattcaggg cctttgcctt tgttacattt gcagatgatc
      781 agattgcgca gtctctttgt ggagaggact tgatcattaa aggaatcagc gttcatatat
      841 ccaatgccga acctaagcac aatagcaata gacagttaga aagaagtgga a
//
