LOCUS       sequence-337931-        4404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC2A12_STOP
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1961..2060)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2077..2096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2078..2096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2101..2117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2101..2117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2219..2238)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2230..3039
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2297..2316)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3186..3774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3675..3694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3928..3945
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4104..4131)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4223..4309)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4358..4380
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4372..4389
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4373..4389
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gcctgtggag
      121 aataccgagg gcccatccct gctgaaccag aagggcaccg ccgtggagac agagggaagc
      181 ggatccaggc acccaccttg ggccagaggc tgcggcatgt tcacctttct gagctccgtg
      241 acagcagccg tgtccggact gctggtggga tacgagctgg gcatcatctc tggcgccctg
      301 ctgcagatca agaccctgct ggccctgagc tgtcacgagc aggagatggt ggtgtctagc
      361 ctggtcatcg gcgccctgct ggcatccctg accggaggcg tgctgatcga cagatatggc
      421 cggagaacag ccatcatcct gtcctcttgc ctgctgggcc tgggctctct ggtgctgatc
      481 ctgtctctga gctacaccgt gctgatcgtg ggccgcatcg ccatcggcgt gtccatctct
      541 ctgagctcca tcgccacatg cgtgtacatc gcagagatcg cacctcagca caggagggga
      601 ctgctggtga gcctgaatga gctgatgatc gtgatcggca tcctgtccgc ctacatctct
      661 aattatgcct tcgccaacgt gtttcacggc tggaagtaca tgttcggact ggtcatccca
      721 ctgggcgtgc tgcaggcaat cgccatgtat ttcctgccac ccagccctcg gttcctggtc
      781 atgaagggac aggagggagc agcatctaag gtgctgggcc ggctgagagc cctgagcgac
      841 accacagagg agctgaccgt gatcaagtct agcctgaagg atgagtacca gtatagcttc
      901 tgggacctgt ttagatccaa ggataacatg aggacccgca tcatgatcgg cctgacactg
      961 gtgttctttg tgcagatcac cggccagcca aatatcctgt tctacgcctc cacagtgctg
     1021 aagtctgtgg gctttcagag caacgaggca gcatctctgg caagcaccgg agtgggcgtg
     1081 gtgaaagtga tctctaccat cccagccaca ctgctggtgg accacgtggg cagcaagaca
     1141 tttctgtgca tcggctcctc tgtgatggcc gcctccctgg tgaccatggg catcgtgaat
     1201 ctgaacatcc acatgaattt cacacacatc tgtaggtccc acaattctat caaccagagc
     1261 ctggacgagt ccgtgatcta cggccccggc aacctgtcca ccaacaataa cacactgcgg
     1321 gatcacttta agggcatcag ctcccactct agatctagcc tgatgcctct gaggaatgac
     1381 gtggataaga ggggagagac cacaagcgcc tccctgctga acgcaggact gagccacacc
     1441 gagtaccaga tcgtgacaga cccaggcgat gtgcccgcct tcctgaagtg gctgagcctg
     1501 gcctccctgc tggtgtatgt ggccgccttt agcatcggcc tgggccctat gccatggctg
     1561 gtgctgtccg agatcttccc aggaggaatc aggggaagag caatggcact gacctcctct
     1621 atgaattggg gcatcaacct gctgatctcc ctgacattcc tgaccgtgac agatctgatc
     1681 ggcctgcctt gggtgtgctt tatctacacc atcatgtctc tggccagcct gctgttcgtg
     1741 gtcatgttta tcccagagac aaagggctgc tccctggagc agatctctat ggagctggcc
     1801 aaggtgaact acgtgaagaa caacatctgt ttcatgagcc accaccagga ggagctggtg
     1861 cccaagcagc ctcagaagcg gaagccacag gagcagctgc tggagtgcaa caagctgtgc
     1921 ggaaggggac agtctaggca gctgagcccc gagacctaag acccagcttt cttgtacaaa
     1981 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     2041 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2101 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2161 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2221 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2281 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2341 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2401 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2461 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2521 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2581 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2641 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2701 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2761 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2821 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2881 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2941 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     3001 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     3061 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3121 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3961 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4081 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4141 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4201 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4261 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4321 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4381 acggccagtc ttaagctcgg gccc
//
