LOCUS       sequence-337935-        5910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC38A10_STOP
FEATURES             Location/Qualifiers
     source          1..5910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(239..256)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     protein_bind    complement(3467..3566)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3583..3602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3584..3602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3607..3623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3607..3623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3725..3744)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3736..4545
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3803..3822)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4692..5280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5181..5200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5434..5451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5610..5637)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5729..5815)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5864..5886
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5878..5895
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5879..5895
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac cgcagcagca
      121 gcaagcaact ggggactgat cacaaacatc gtgaattcta tcgtgggcgt gagcgtgctg
      181 accatgccct tctgctttaa gcagtgtgga atcgtgctgg gcgccctgct gctggtgttt
      241 tgctcttgga tgacccacca gagctgtatg ttcctggtga agtctgccag cctgtccaag
      301 cggagaacat acgccggcct ggcctttcat gcgtatggaa aggcggggaa gatgctggtg
      361 gagaccagca tgatcggcct gatgctgggc acatgcatcg ccttctatgt ggtcatcggc
      421 gacctgggct ccaacttctt tgccagactg ttcggctttc aagtgggcgg caccttcagg
      481 atgtttctgc tgttcgccgt gagcctgtgc atcgtgctgc ctctgtccct gcagagaaat
      541 atgatggcct ctatccagtc ttttagcgcc atggccctgc tgttctacac agtgttcatg
      601 tttgtgatcg tgctgagctc cctgaagcac ggcctgttta gcggccagtg gctgaggcgc
      661 gtgtcctatg tgcgctggga gggcgtgttc cggtgcatcc caatctttgg catgtccttc
      721 gcctgtcagt ctcaggtgct gcccacctac gacagcctgg atgagccttc cgtgaagaca
      781 atgtctagca tctttgcctc ctctctgaat gtggtgacca cattctacgt gatggtgggc
      841 ttctttggct atgtgagctt taccgaggcc acagccggca acgtgctgat gcacttcccc
      901 tccaatctgg tgaccgagat gctgcgggtg ggctttatga tgtctgtggc cgtgggcttc
      961 cccatgatga tcctgccttg cagacaggcc ctgtccaccc tgctgtgcga gcagcagcag
     1021 aaggacggca catttgccgc aggaggatat atgccacctc tgcggtttaa ggccctgacc
     1081 ctgagcgtgg tgttcggcac aatggtgggc ggcatcctga tcccaaacgt ggagaccatc
     1141 ctgggactga ccggagcaac aatgggatcc ctgatctgct tcatctgtcc cgccctgatc
     1201 tacaagaaga tccacaagaa tgccctgagc tcccaggtgg tgctgtgggt gggactgggc
     1261 gtgctggtgg tgagcacagt gaccacactg tccgtgtctg aggaagtgcc agaggatctg
     1321 gcagaggagg caccaggagg cagactgggc gaggcagagg gactgatgaa ggtggaggca
     1381 gccaggctgt ccgcccagga cccagtggtg gcagtggcag aggatggccg cgagaagcca
     1441 aagctgccaa aggagaggga ggagctggag caggcccaga tcaagggacc tgtggacgtg
     1501 ccaggaaggg aggatggcaa ggaggcacct gaggaggcac agctggacag gccaggccag
     1561 ggcatcgccg tgcccgtggg cgaggcccac agacacgagc cacccgtgcc tcacgataag
     1621 gtggtggtgg acgagggaca ggatagggag gtgccagagg agaacaagcc tccatctagg
     1681 cacgcaggag gcaaggcacc aggagtgcag ggccagatgg ccccccctct gccagacagc
     1741 gagagggaga agcaggagcc tgagcagggc gaagtgggca agcggccagg acaggcacag
     1801 gccctggagg aggcaggcga cctgcctgag gatccacaga aggtgcctga ggcagatgga
     1861 cagccagcag tgcagcctgc aaaggaggac ctgggaccag gcgatagagg actgcaccca
     1921 aggcctcagg ccgtgctgtc cgagcagcag aacggactgg cagtgggagg aggagagaag
     1981 gccaagggag gaccaccacc tggaaatgca gcaggcgaca ccggccagcc agccgaggac
     2041 tctgatcacg gcggcaagcc accactgcca gcagagaagc cagcaccagg acctggcctg
     2101 cctccagagc caagggagca gagggatgtg gagagggcag gaggaaatca ggcagcaagc
     2161 cagctggagg aggcaggacg cgccgagatg ctggaccacg ccgtgctgct gcaggtcatc
     2221 aaggagcagc aggtgcagca gaagcggctg ctggatcagc aggagaagct gctggccgtg
     2281 atcgaggagc agcacaagga gatccaccag cagaggcagg aggacgagga ggataagcca
     2341 aggcaggtgg aggtgcacca ggagccagga gcagcagtgc caaggggaca ggaggcacct
     2401 gagggcaagg caagggagac cgtggagaac ctgccacctc tgccactgga ccctgtgctg
     2461 agggcaccag gaggccggcc agcaccctcc caggatctga atcagagaag cctggagcac
     2521 tccgagggcc ctgtgggcag ggacccagca ggaccacctg acggaggacc agataccgag
     2581 cccagagcag cacaggcaaa gctgagggac ggacagaagg atgcagcacc aagggcagcc
     2641 ggcacagtga aggagctgcc aaagggacca gagcaggtgc ctgtgccaga tccagcaaga
     2701 gaggcaggag gaccagagga gaggctggca gaggagttcc ctggacagtc ccaggacgtg
     2761 accggaggct ctcaggatcg caagaagcct ggcaaggagg tggcagcaac cggcacatcc
     2821 atcctgaagg aggcaaactg gctggtggca ggaccaggag cagagacagg cgaccctcgc
     2881 atgaagccaa agcaggtgtc tcgggacctg ggcctggccg ccgatctgcc aggaggagca
     2941 gagggagcag cagcacagcc tcaggccgtg ctgagacagc cagagctgag agtgatctct
     3001 gacggagagc agggaggaca gcagggacac aggctggatc acggcggcca cctggagatg
     3061 agaaaggcaa ggggaggcga ccacgtgccc gtgagccacg agcagcctag gggaggcgag
     3121 gatgccgccg tgcaggagcc tagacagagg cctgagccag agctgggcct gaagagggca
     3181 gtgccaggag gacagaggcc tgacaacgcc aagccaaatc gggatctgaa gctgcaggca
     3241 ggaagcgacc tgcggagaag gcgccgggat ctgggacctc acgcagaggg acagctggca
     3301 ccaagagacg gcgtgatcat cggcctgaac cccctgcctg acgtgcaagt gaatgatctg
     3361 agaggcgccc tggatgcaca gctgaggcag gcagcaggag gcgccctgca ggtggtgcac
     3421 tctcgccagc tgcggcaggc ccccggccct ccagaggaga gctaagaccc agctttcttg
     3481 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     3541 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     3601 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     3661 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     3721 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     3781 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     3841 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     3901 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     3961 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     4021 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     4081 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     4141 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     4201 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     4261 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     4321 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     4381 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     4441 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     4501 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     4561 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     4621 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     4681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5461 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     5521 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     5581 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     5641 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     5701 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     5761 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     5821 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     5881 aaaacgacgg ccagtcttaa gctcgggccc
//
