LOCUS       sequence-337936-        4116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35F4_STOP
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1673..1772)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1789..1808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1790..1808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1813..1829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1813..1829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1931..1950)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1942..2751
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2009..2028)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2898..3486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3387..3406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3640..3657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3816..3843)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3935..4021)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4070..4092
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4084..4101
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4085..4101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga cgagctgctg
      121 ctggatctgt tccacaagct gaccagcggc aggcagctgg cagcaggaaa cggactgtgc
      181 ggcatctccc acaaggagca ggaagtgtgg aagcctggcc acaatatcct ggtgaagatg
      241 aggaaggagg acaagtccct ggtgtggctg atccactcta ccctggcccg ctacacccag
      301 gtgacaaact ttctgggcac ctccaggagc tccgtgacaa ggtgcaagcc aggagccaat
      361 tgtccttcta gccacagcgg catctcccgc cagctgtctc cactgagcgt gacagaggat
      421 tcctctgccc ccatcctgga gctgcagaac cagggcagct ccggcgtgtg cggacacagg
      481 gtggagaggc agaatagaag cgccgacgat ggcacccaga cacactccga gaactctagc
      541 caggagaata ggatcaaggc ccgctgcctg tcctgtacct ctatggtgct gaagggcatc
      601 tggggcctgc tgatcatcct gagcgtgtcc tctagctggg tgggcaccac acagatcgtg
      661 aagatcacat acaagaactt ctattgcccc ttctttatga cctggttttc cacaaactgg
      721 aatatcatgt tctttcccgt gtactattct ggacacctgg caaccgcaca ggagaagcag
      781 tctccaatga agaagttccg ggagtgtagc agaatctttg gcgaggacgg cctgaccctg
      841 aagctgttcc tgaagcggac agccccattt tccatcctgt ggaccctgac aaactacctg
      901 tatctgctgg ccctgaagaa gctgaccgcc acagacgtgt ctgccctgtt ctgctgtaat
      961 aaggccttcg tgtttctgct gagctggatc gtgctgaagg atcggtttat gggcgtgaga
     1021 atcgtggccg ccatcatggc catcaccggc atcgtgatga tggcctacgc cgacaatttc
     1081 cacgccgata gcatcatcgg agtggccttt gccgtgggaa gcgcctccac ctctgccctg
     1141 tataaggtgc tgttcaagat gtttctggga tccgccaact tcggagaggc agcacacttc
     1201 gtgagcaccc tgggcttctt taatctgatc ttcatcagct ttaccccagt gatcctgtac
     1261 ttcacaaagg tggagcactg gtcctctttt gccgccctgc catggggatg cctgtgcgga
     1321 atggccggcc tgtggctggc cttcaacatc ctggtgaatg tgggcgtggt gctgacctat
     1381 cctatcctga tcagcatcgg cacagtgctg tccgtgccag gaaacgcagc agtggatctg
     1441 ctgaagcagg aagtgatctt caacgtggtg cggctggccg ccaccatcat catctgcatc
     1501 ggctttctgc tgatgctgct gcctgaggag tgggacgaga tcacactgag attcatcaac
     1561 tctctgaagg agaagaagag cgaggagcac gtggacgatg tgaccgatcc atccatccac
     1621 ctgaggggaa gaggaagggc aaatggaaca gtgagcatcc ctctggccta agacccagct
     1681 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1741 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1801 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1861 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1921 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1981 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2041 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2101 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2161 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2221 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2281 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2341 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2401 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2461 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2521 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2581 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2641 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2701 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2761 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2821 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3661 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3721 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3781 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3841 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3901 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3961 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     4021 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4081 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
