LOCUS       sequence-337937-        3801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35D3_STOP
FEATURES             Location/Qualifiers
     source          1..3801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1358..1457)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1474..1493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1475..1493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1498..1514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1498..1514)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1616..1635)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1627..2436
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1694..1713)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2583..3171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3072..3091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3325..3342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3501..3528)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3620..3706)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3755..3777
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3769..3786
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3770..3786
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag gcagctgtgc
      121 aggggaagag tgctgggaat ctccgtggca atcgcacacg gcgtgttcag cggatccctg
      181 aacatcctgc tgaagtttct gatcagcaga taccagttct cctttctgac cctggtgcag
      241 tgcctgacca gctccacagc cgccctgtcc ctggagctgc tgcggagact gggactgatc
      301 gcagtgccac ctttcggcct gtccctggca aggtcttttg ccggagtggc cgtgctgtct
      361 accctgcagt ctagcctgac actgtggtct ctgaggggac tgagcctgcc aatgtatgtg
      421 gtgttcaaga gatgtctgcc tctggtgaca atgctgatcg gcgtgctggt gctgaagaat
      481 ggagcaccaa gcccaggcgt gctggcagcc gtgctgatca ccacatgcgg agccgccctg
      541 gcaggagcag gcgacctgac cggcgatccc atcggctacg tgacaggcgt gctggccgtg
      601 ctggtgcacg cagcatatct ggtgctgatc cagaaggcaa gcgccgacac cgagcacgga
      661 ccactgacag cccagtacgt gatcgccgtg tccgccaccc cactgctggt catctgttct
      721 ttcgccagca ccgactccat ccacgcctgg acatttccag gctggaagga tcccgccatg
      781 gtgtgcatct tcgtggcctg tatcctgatc ggctgcgcca tgaactttac cacactgcac
      841 tgcacatata tcaattccgc cgtgaccaca tcttttgtgg gcgtggtgaa gtccatcgcc
      901 accatcacag tgggcatggt ggccttcagc gatgtggagc ctacctccct gtttatcgcc
      961 ggcgtggtgg tgaacacact gggctccatc atctactgcg tggccaagtt catggagacc
     1021 aggaagcagt ctaattatga ggacctggag gcacagccta ggggagagga ggcccagctg
     1081 tctggcgatc agctgccttt tgtgatggag gagctgccag gagagggagg aaacggcaga
     1141 agcgagggag gagaggcagc aggaggacca gcccaggagt ctagacagga ggtgagggga
     1201 agcccaaggg gagtgcctct ggtggcagga tcctctgagg agggatctag gcgcagcctg
     1261 aaggacgcct acctggaagt gtggaggctg gtgcgcggaa cccggtacat gaagaaggat
     1321 tatctgatcg agaatgagga gctgcctagc ccataagacc cagctttctt gtacaaagtt
     1381 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1441 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1501 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1621 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1681 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1741 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1801 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1861 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1921 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1981 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2041 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2101 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2161 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2221 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2281 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2341 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2401 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2461 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2521 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3241 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3301 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3361 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3421 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3481 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3541 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3601 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3661 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3721 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3781 gccagtctta agctcgggcc c
//
