LOCUS       sequence-337940-       15187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector with TUs incorporated. Cells will be red and, if 
            treated with doxycycline, blue..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15187)
  TITLE     Leonard Lab mMoClo Plasmids
REFERENCE   2  (bases 1 to 15187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD1101 Integration Test Circuit Vector
FEATURES             Location/Qualifiers
     source          1..15187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1676..1732
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1733..2410
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(1913..1933)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2325..2348
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2523..2542)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2588..2643
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2642..2661)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3712..4061
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    3720..3738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3756..3774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3792..3810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3828..3846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3864..3882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3900..3918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3936..3954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4106..4124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4144..4153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4914..5138
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6187..7369
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6418..7360
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7317..7337
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7470..7479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8457..8513
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8526..8924
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8975..9199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10097..10476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          10752..11768
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     11692..11714
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11776..11795
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        11869..11887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11913..12659
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    12710..12934
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(13793..14602)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     14516..14535
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     14594..14613
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(14761..15187,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14841..14860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gagctgccac
     1681 gaacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ccatggatag
     1741 cactgagaac gtcatcaagc ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1801 cggccacgag ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac
     1861 cgccaagctc caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     1921 ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa
     1981 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     2041 cgtggtgacc gtgacccagg actcctccct ccaggacggc accttcatct accacgtgaa
     2101 gttcatcggc gtgaacttcc cctccgacgg ccccgtaatg cagaagaaaa ctctgggctg
     2161 ggagccctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacaa
     2221 ggcgctgaag ctgaagggcg gcggccacta cctggtggag ttcaagtcaa tctacatggc
     2281 caagaagccc gtgaagctgc ccggctacta ctacgtggac tccaagctgg acatcacctc
     2341 ccacaacgag gactacaccg tggtggagca gtacgagcgc gccgaggccc gccaccacct
     2401 gttccagtag gcttccactg gattgtacaa ttaccaactg aattcactcc tcaggtgcag
     2461 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     2521 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     2581 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     2641 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     2701 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     2761 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     2821 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     2881 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     2941 atagctgtcc ctcttctctt atggagatcc gggcctagca tcgcgataag ctctagaggg
     3001 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     3061 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     3121 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     3181 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     3241 aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg gctctagctc
     3301 tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc ccaaagcccc
     3361 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     3421 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     3481 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     3541 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     3601 aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg agattgccaa
     3661 ggtcttgaca aacgagtgct agtacaagat caggcccttt catcttcact cgagtttact
     3721 ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata gagaacgtat
     3781 gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc ctatcagtga
     3841 tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct acagtttact
     3901 ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat
     3961 gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
     4021 gatcgcctgg agcaattcca caacactttt gtcttatact tctctggcta actagagaac
     4081 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagtcc caagctggct
     4141 agcgccacca tggtttccaa gggcgaagaa ctgatcaaag aaaacatgca catgaagctc
     4201 tacatggaag gcaccgtgga caaccaccac ttcaagtgta ccagcgaagg cgagggcaag
     4261 ccttacgagg gcacccagac catgagaatc aaggtggtgg aaggcggccc tctgcctttc
     4321 gcctttgata tcctggccac cagctttctg tacggcagca agaccttcat caatcacacc
     4381 cagggcatcc ccgatttctt caagcagagc ttccccgagg gcttcacctg ggagagagtg
     4441 accacatacg aggatggcgg cgtgctgaca gccacacagg atacaagcct ccaggacggc
     4501 tgcctgatct acaacgtgaa gatccggggc gtgaacttca ccagcaacgg ccccgtgatg
     4561 cagaagaaaa cccttggctg ggaagccttc accgagacac tgtatcctgc cgatggcggc
     4621 ctggaaggca gaaacgatat ggccctgaag ctcgtcggcg gctctcacct gattgccaat
     4681 gccaagacca cctacagaag caagaagccc gccaagaacc tgaagatgcc cggcgtgtac
     4741 tacgtggact accggctgga aagaatcaaa gaggccaaca acgagacata cgtggaacag
     4801 cacgaggtgg ccgtggccag atactgtgat ctgccttcta agctgggcca caagctgaac
     4861 tgagcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4921 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4981 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5041 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaagt
     5101 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcag gtcgagggta
     5161 catgcgcctt actccttgtg agcgcatcgc gataagctct agagggacag ccccccccca
     5221 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     5281 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc
     5341 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     5401 agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga
     5461 atgacagaat catagaacgg cctgcgtacg gatggggctc tagctctaga gcttgatatc
     5521 gaattcctgc agccccgcta gagggacagc ccccccccaa agcccccagg gatgtaatta
     5581 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
     5641 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
     5701 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
     5761 atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc
     5821 ctgggcgcgc ctggtggcga acgatcaaca gacgggagag ggcaaaaccg atgttgacgg
     5881 actaatcctg acaagatctg gggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     5941 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     6001 gctgcttcgc gatgtacggg ccagatatac gcgtgagtaa ttcatacaaa aggactcgcc
     6061 cctgccttgg ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat
     6121 cgctgcgttc ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat
     6181 gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6241 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     6301 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6361 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     6421 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     6481 tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     6541 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     6601 tgaggcctgg cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     6661 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     6721 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     6781 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     6841 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     6901 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     6961 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     7021 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     7081 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     7141 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     7201 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     7261 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     7321 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agctcgttta
     7381 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagagaa
     7441 cgggaccgat ccaaattctc gaggctagcg ccaccatggt tgccggccac gcttctggat
     7501 ctccagcctt tggaacagcc agccacagca actgcgagca cgaggaaatt cacctggccg
     7561 gctctattca gcctcatggc gctctgctgg tggtgtccga acacgaccac agagtgattc
     7621 aggcctctgc caatgccgcc gagttcctga atctgggctc tgtgctggga gtgcctctgg
     7681 ccgaaattga cggcgacctg ctgatcaaga tcctgcctca tctggaccct accgccgagg
     7741 gaatgcctgt ggccgtcaga tgcagaatcg gcaaccccag caccgagtat tgcggcctga
     7801 tgcacagacc tccagaaggc ggactgatca tcgagctgga aagagctggc cccagcatcg
     7861 acctgtctgg aacacttgct cccgctctgg aacggatcag aacagccgga tctctgagag
     7921 ccctgtgcga cgataccgtg ctgctgttcc agcagtgtac cggctacgat agagtgatgg
     7981 tgtatcgctt cgacgagcaa ggccacggcc tggtgtttag cgaatgtcac gtgcccggcc
     8041 tggaaagcta cttcggcaac agatacccca gcagcaccgt gcctcagatg gccagacagc
     8101 tgtatgtgcg gcagcgcgtt agagtgctgg tggacgtgac ataccagcct gtgccactgg
     8161 aacccagact gagccctctg acaggccgcg atctggatat gagcggctgt ttcctgcgga
     8221 gcatgagccc atgccacctc cagtttctga aggacatggg cgtcagagcc acactggccg
     8281 tgtctcttgt tgttggcgga aagctgtggg gcctcgtcgt gtgtcaccac tacctgcctc
     8341 ggttcatcag attcgagctg cgggccatct gcaagagact ggccgagaga atcgccacca
     8401 gaatcacagc cctggaatcc ctgtacaagg aattcggtag caagcttggt agtggagcaa
     8461 ctaacttttc cctgctcaaa caagccgggg atgtagagga gaaccccggc cccgcccccc
     8521 ttaagatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     8581 caatcaacag catccccatc tctgaggact acagcgtcgc cagcgcagct ctctctagcg
     8641 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     8701 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     8761 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     8821 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     8881 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcggcc gctcgagtct
     8941 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9061 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9121 ggtggggtgg ggcaggacag caagggggag gattgggaag tcaatagcag gcatgctggg
     9181 gatgcggtgg gctctatggc ttctgaggca ggtcgaagaa ctatttcctg gctgttacgc
     9241 gagcgcatcg cgataagctc tagagggaca gccccccccc aaagccccca gggatgtaat
     9301 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     9361 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     9421 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     9481 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     9541 gcctgcgtac ggatggggct ctagctctag agcttgatat cgaattcctg cagccccgct
     9601 agagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
     9661 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
     9721 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
     9781 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta
     9841 ggctgaaaga gagatttaga atgacagaat catagaacgg cctgggcgcg cctggtggcg
     9901 aacgatcaac agacgggaga ggactaaata gtagttcaga cgccgttaag cgcaagatct
     9961 ggggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    10021 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
    10081 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
    10141 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
    10201 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
    10261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
    10321 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
    10381 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
    10441 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
    10501 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
    10561 ttattttgtg cagcgatggg ggcggggggg gggggggsgc gccaggcggg gcggggcggg
    10621 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
    10681 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
    10741 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
    10801 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
    10861 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
    10921 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
    10981 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
    11041 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
    11101 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
    11161 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
    11221 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
    11281 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
    11341 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc
    11401 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
    11461 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
    11521 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
    11581 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
    11641 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
    11701 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
    11761 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
    11821 tcttatctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
    11881 ctatagggag tcccaagctg gctagcgcca ccatgtctag actggacaag agcaaagtca
    11941 taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
    12001 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
    12061 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc
    12121 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
    12181 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
    12241 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
    12301 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
    12361 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
    12421 tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
    12481 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
    12541 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
    12601 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaag
    12661 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
    12721 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    12781 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    12841 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagtcaat
    12901 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcaggtcg aagagactgc
    12961 ccgacacatc ttagtgagcg catcgcgata agctctagag ggacagcccc cccccaaagc
    13021 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
    13081 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
    13141 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
    13201 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
    13261 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
    13321 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
    13381 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
    13441 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
    13501 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
    13561 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
    13621 gcgcgcctgg tggcgaacga tcaacagacg ggagaggtta cgaggatgca catgtgaccg
    13681 agggatggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc
    13741 gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa
    13801 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
    13861 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
    13921 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
    13981 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
    14041 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
    14101 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
    14161 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
    14221 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
    14281 atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag
    14341 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
    14401 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
    14461 gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc
    14521 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg
    14581 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
    14641 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
    14701 cgggccatgc tagctccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    14761 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    14821 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    14881 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    14941 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    15001 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    15061 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    15121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    15181 taactac
//
