LOCUS       sequence-33795-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pCold TDP43 Q331K
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..18
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..48
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cccatgaatc acaaagtgca tcatcatcat catcatatcg aaggtaggca tatggagctc
       61 ggtaccctcg agggatccat gtctgaatat attcgggtaa ccgaagatga gaacgatgag
      121 cccattgaaa taccatcgga agacgatggg acggtgctgc tctccacggt tacagcccag
      181 tttccagggg cgtgtgggct tcgctacagg aatccagtgt ctcagtgtat gagaggtgtc
      241 cggctggtag aaggaattct gcatgcccca gatgctggct ggggaaatct ggtgtatgtt
      301 gtcaactatc caaaagataa caaaagaaaa atggatgaga cagatgcttc atcagcagtg
      361 aaagtgaaaa gagcagtcca gaaaacatcc gatttaatag tgttgggtct cccatggaaa
      421 acaaccgaac aggacctgaa agagtatttt agtacctttg gagaagttct tatggtgcag
      481 gtcaagaaag atcttaagac tggtcattca aaggggtttg gctttgttcg ttttacggaa
      541 tatgaaacac aagtgaaagt aatgtcacag cgacatatga tagatggacg atggtgtgac
      601 tgcaaacttc ctaattctaa gcaaagccaa gatgagcctt tgagaagcag aaaagtgttt
      661 gtggggcgct gtacagagga catgactgag gatgagctgc gggagttctt ctctcagtac
      721 ggggatgtga tggatgtctt catccccaag ccattcaggg cctttgcctt tgttacattt
      781 gcagatgatc agattgcgca gtctctttgt ggagaggact tgatcattaa aggaatcagc
      841 gttcatatat ccaatgccga acctaagcac aatagcaata gacagttaga aagaagtgga
      901 aga
//
