LOCUS       sequence-337971-       10902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  deletion of a genomic locus in Yap1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Kagiwada S, Aramaki S, Wu G, Shin B, Kutejova E, Obridge D, Adachi 
            K, Wrana JL, Hubner K, Scholer HR
  TITLE     YAP establishes epiblast responsiveness to inductive signals for 
            germ cell fate.
  JOURNAL   Development. 2021 Oct 15;148(20). pii: 272520. doi: 
            10.1242/dev.199732. Epub 2021 Oct 19.
  PUBMED    34528691
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2021-10-15"; volume: "148(20). pii: 272520. 
            doi"; pages: " 10.1242/dev.199732. Epub 2021 Oct 19"
COMMENT     Sequence Label: pX330A-Yap1-E1-#1
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          139..366
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      378..387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             387..452
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             459..479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             504..4604
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             504..4604
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4605..4652
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4680..4697)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4686..4893
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4902..5042
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4902..5031
                     /label=AAV2 ITR
     promoter        5187..5398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5411..5679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5708..5717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5738..6400
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6470..6523
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6524..7123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6524..6543)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7020..7040
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7177..7197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7239..7360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7276..7295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7330..7349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7511..7966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7598..7617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7808..7829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7983..8002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8102..8124
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8162..8180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8248..8352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8353..8421,8422..9213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8571..8590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9384..9972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9873..9892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10034..10282
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10034..10054
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10205..10224
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10304..10379
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        10476..10761
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(10763..10902,1..138)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggsggggsg rggggsgggg sggggcgagg cggagaggtg cggcggcagc caatcagagc
       61 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      121 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      181 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      241 cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga
      301 tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt
      361 tttcaggttg gaccggtgcc accatggact ataaggacca cgacggagac tacaaggatc
      421 atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg
      481 gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca
      541 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
      601 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
      661 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
      721 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
      781 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
      841 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
      901 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
      961 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     1021 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     1081 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     1141 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     1201 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     1261 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     1321 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1381 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1441 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1501 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     1561 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     1621 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     1681 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     1741 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     1801 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     1861 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     1921 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     1981 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     2041 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     2101 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     2161 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     2221 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     2281 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     2341 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2401 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2461 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     2521 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     2581 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     2641 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     2701 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     2761 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     2821 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     2881 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     2941 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     3001 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca
     3061 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     3121 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     3181 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     3241 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     3301 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     3361 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3421 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3481 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     3541 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     3601 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     3661 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     3721 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     3781 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
     3841 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     3901 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     3961 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     4021 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     4081 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     4141 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     4201 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     4261 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     4321 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     4381 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     4441 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
     4501 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     4561 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg ccggcggcca
     4621 cgaaaaaggc cggccaggca aaaaagaaaa agtaagaatt cctagagctc gctgatcagc
     4681 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4741 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4801 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4861 ggattgggaa gagaatagca ggcatgctgg ggagcggccg caggaacccc tagtgatgga
     4921 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4981 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     5041 ggaaagagcc cacaacccct cacttggtgg gccagtcctc tgatagactg tgtcccctgg
     5101 atacccgtat tctagagcta gcgaattcga atttaaatcg gatccgcggc cgcgaaggat
     5161 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5221 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5281 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5341 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5401 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5461 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5521 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     5581 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     5641 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     5701 gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
     5761 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
     5821 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
     5881 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
     5941 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
     6001 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
     6061 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
     6121 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
     6181 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
     6241 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
     6301 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
     6361 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc
     6421 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg
     6481 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc
     6541 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6601 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6661 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6721 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6781 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6841 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6901 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6961 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7021 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7081 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta
     7141 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7201 atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt gcagcttata
     7261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg aattgactca
     7381 aatgatgtca attagtctat cagaagctca tctggtctcc cttccgggcc tgcaggggcg
     7441 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     7501 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7561 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     7621 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7681 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     7741 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7801 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     7861 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     7921 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     7981 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     8041 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8101 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8161 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8221 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8281 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8341 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8401 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8461 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8521 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8581 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8641 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8701 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8761 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8821 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8881 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8941 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9001 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9061 gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9121 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9181 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9241 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9301 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10021 tttgctcaca tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
    10081 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
    10141 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
    10201 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
    10261 cttgtggaaa ggacgaaaca ccgcgcggcc ttggcgttgc gcggttttag agctagaaat
    10321 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
    10381 tttttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg
    10441 cgccaattct gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc
    10501 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg
    10561 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10621 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10681 tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10741 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
    10801 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
    10861 atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sg
//
