LOCUS       sequence-337986-        7769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1-alpha 
            promoter (1.1kb short version)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, 
            Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, 
            Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y,
            Tye KM, Roska B, Cardin JA, Boyden ES
  TITLE     Noninvasive optical inhibition with a red-shifted microbial 
            rhodopsin.
  JOURNAL   Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014
            Jul 6.
  PUBMED    24997763
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.3752"; 
            journalName: "Nat Neurosci"; date: "2014-08"; volume: "17"; issue: 
            "8"; pages: "1123-9"
COMMENT     Sequence Label: pAAV-EF1-alpha-1.1-Jaws-KGC-tdTomato-ER2
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        168..1346
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          399..1337
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1294..1314
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3756..4344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3809..3829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4227..4238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4364..4840
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4563..4582)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4886..5026
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4886..5015
                     /label=AAV2 ITR
     rep_origin      5101..5556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5188..5207)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5398..5419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5573..5592)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5692..5714
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5752..5770)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5838..5942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5943..6011,6012..6803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6161..6180)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6974..7562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7463..7482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7624..7753
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
       61 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      121 gtccccgaga agttgggggg aggggtcggc agcaaatggt taattaaggc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      421 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      781 gggcccgtgc gtcccagcgc acatgttcga cgaggcgggg cctgcgagcg cggccaccga
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1321 tttttcttcc atttcaggtg tcgtgaggat ccgccaccat gaccgccgtg agcaccacag
     1381 ccactaccgt gctgcaggcc acacagagcg acgtgctgca ggagatccag tccaacttcc
     1441 tgctgaatag ctccatctgg gtgaacattg ctctggccgg agtggtcatc ctgctgtttg
     1501 tggccatggg gagggatctg gaatccccta gagctaagct gatctgggtg gccacaatgc
     1561 tggtgccact ggtgtctatt tctagttacg ctggactggc cagtgggctg actgtgggct
     1621 tcctgcagat gccacctgga cacgctctgg ccggacagga ggtgctgagc ccatggggcc
     1681 ggtatctgac atggactttc tccactccca tgatcctgct ggctctggga ctgctggccg
     1741 acaccgatat tgccagcctg ttcaccgcca tcacaatgga cattggcatg tgcgtgacag
     1801 gactggccgc tgccctgatc actagctccc atctgctgcg ctgggtgttc tacggaattt
     1861 cttgtgcttt ctttgtggcc gtgctgtatg tgctgctggt gcagtggcca gctgatgctg
     1921 aggctgctgg gaccagtgaa atctttggca ctctgaggat tctgaccgtg gtgctgtggc
     1981 tggggtaccc tatcctgttc gctctgggct ctgagggagt ggccctgctg agtgtgggag
     2041 tgaccagctg gggatactcc ggactggaca tcctggctaa atacgtgttc gcctttctgc
     2101 tgctgagatg ggtggctgcc aatgaaggca cagtgtctgg gagtggaatg ggaatcgggt
     2161 ccggaggagc tgctccagcc gacgatcgac cggtagtaaa atccagaatt acttctgaag
     2221 gggagtatat ccctctggat caaatagaca tcaatgttgc acctgcaggg gcagtgagta
     2281 agggcgagga agtgatcaaa gagttcatgc ggtttaaggt gagaatggaa ggaagcatga
     2341 acggccacga gttcgaaatt gagggagaag gagagggacg gccctacgag ggcacccaga
     2401 cagccaagct gaaagtgaca aagggcgggc ctctgccatt cgcttgggac atcctgagcc
     2461 cacagtttat gtacggctcc aaggcctatg tgaaacatcc agctgacatt cccgattata
     2521 agaaactgag cttccccgag gggtttaagt gggaaagagt gatgaacttc gaggacggag
     2581 gcctggtgac tgtgacccag gacagctccc tgcaggatgg gaccctgatc tacaaggtga
     2641 aaatgagagg gacaaatttt ccccctgatg gacctgtgat gcagaagaaa actatgggat
     2701 gggaggcctc caccgaaagg ctgtatccac gcgacggggt gctgaaagga gaaatccacc
     2761 aggctctgaa gctgaaagat gggggacatt acctggtgga gttcaagaca atctacatgg
     2821 ccaagaaacc tgtgcagctg ccaggctact attacgtgga cacaaaactg gatatcactt
     2881 cacacaacga ggactacact attgtggagc agtatgaacg gagcgagggg agacaccatc
     2941 tgttcctggg ccatgggact ggaagtaccg gctcagggtc tagtggaacc gcctcaagcg
     3001 aggataacaa tatggctgtg atcaaagagt tcatgaggtt taaggtgcgc atggagggca
     3061 gcatgaatgg gcacgaattt gagattgaag gagagggcga agggaggcct tacgagggca
     3121 cacagactgc caagctgaaa gtgaccaagg gaggaccact gcctttcgct tgggatatcc
     3181 tgtctcctca gtttatgtac ggaagtaagg cctatgtcaa gcatcccgct gacattcctg
     3241 attacaagaa actgtctttc ccagagggct ttaagtggga gagagtgatg aattttgaag
     3301 atggaggcct ggtgaccgtg acacaggact cctctctgca ggatggcact ctgatctaca
     3361 aagtcaaaat gcgcggcacc aattttccac ccgatgggcc cgtgatgcag aagaaaacaa
     3421 tggggtggga ggccagcact gaacggctgt atcctagaga cggagtgctg aagggcgaaa
     3481 tccaccaggc cctgaagctg aaagacggcg gccactacct ggtggagttc aaaaccatct
     3541 acatggccaa gaaaccagtg cagctgcccg gctattacta tgtggacacc aagctggata
     3601 tcacatccca caatgaagac tacaccattg tggaacagta tgagaggtct gaaggacgcc
     3661 accatctgtt tctgtacggc atggatgagc tgtataaggt agcattctgc tacgagaatg
     3721 aagtgtaata ggaattccgt acaaagctta tcgataatca acctctggat tacaaaattt
     3781 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3841 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3901 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3961 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4021 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4081 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4141 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     4201 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4261 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4321 tctccctttg ggccgcctcc ccgcatcgat accggctcga gacgggtggc atccctgtga
     4381 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt
     4441 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg
     4501 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg
     4561 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc
     4621 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg
     4681 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc
     4741 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct
     4801 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctcgagctga ttttgtaggt
     4861 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     4921 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4981 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     5041 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     5101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5161 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5221 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5281 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     5341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5401 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     5461 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     5521 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     5581 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5641 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5701 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5761 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5821 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5881 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5941 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6001 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6061 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6121 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6181 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6241 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6301 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6361 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6421 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6481 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6541 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6601 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6661 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6721 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6781 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6841 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7081 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7621 tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc
     7681 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     7741 actaggggtt cctgcggccg cacgcgttg
//
