LOCUS       sequence-337987-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full-length, human GIRK4 with C-terminal StrepTag II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Qiao P, Schrecke S, Lyu J, Zhu Y, Zhang T, Benavides A, Laganowsky A
  TITLE     Insight into the Phospholipid-Binding Preferences of Kir3.4.
  JOURNAL   Biochemistry. 2021 Dec 21;60(50):3813-3821. doi: 
            10.1021/acs.biochem.1c00615. Epub 2021 Nov 30.
  PUBMED    34846128
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.1c00615"; journalName: "Biochemistry"; date: 
            "2021-12-21- 21"; volume: "60"; issue: "50"; pages: "3813-3821"
COMMENT     Sequence Label: pACE-GIRK4-S
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1482..1505
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1532..1666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1569..1588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1623..1642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1787..1952
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2142..2730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2631..2650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2865..3088
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3155..3688)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3877..3882,3883..3899,3900..3905))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa ataaccgcca ccatggccgg cgattctagg aatgccatga
      241 accaggacat ggagattgga gtcactccct gggaccccaa gaagattcca aaacaggccc
      301 gcgattatgt ccccattgcc acagaccgta cgcgcctgct ggccgagggc aagaagccac
      361 gccagcgcta catggagaag agcggcaagt gcaacgtgca ccacggcaac gtccaggaga
      421 cctaccggta cctgagtgac ctcttcacca ccctggtgga cctcaagtgg cgcttcaact
      481 tgctcgtctt caccatggtt tacactgtca cctggctgtt cttcggcttc atttggtggc
      541 tcattgctta tatccggggt gacctggacc atgttggcga ccaagagtgg attccttgtg
      601 ttgaaaacct cagtggcttc gtgtccgctt tcctgttctc cattgagacc gaaacaacca
      661 ttgggtatgg cttccgagtc atcacagaga agtgtccaga ggggattata ctcctcttgg
      721 tccaggccat cctgggctcc atcgtcaatg ccttcatggt ggggtgcatg tttgtcaaga
      781 tcagccagcc caagaagaga gcggagaccc tcatgttttc caacaacgca gtcatctcca
      841 tgcgggacga gaagctgtgc ctcatgttcc gggtgggcga cctccgcaac tcccacatcg
      901 tggaggcctc catccgggcc aagctcatca agtcccggca gaccaaagag ggggagttca
      961 tccccctgaa ccagacagac atcaacgtgg gctttgacac gggcgacgac cgcctcttcc
     1021 tggtgtctcc tctgatcatc tcccacgaga tcaacgagaa gagccctttc tgggagatgt
     1081 ctcaggctca gctgcatcag gaagagtttg aagttgtggt cattctagaa gggatggtgg
     1141 aagccacagg catgacctgc caagcccgga gctcctacat ggatacagag gtgctctggg
     1201 gccaccgatt cacaccagtc ctcaccttgg aaaagggctt ctatgaggtg gactacaaca
     1261 ccttccatga tacctatgag accaacacac ccagctgctg tgccaaggag ctggcagaaa
     1321 tgaagaggga aggccggctc ctccagtacc tccccagccc cccactgctg gggggctgtg
     1381 ctgaggcagg gctggatgca gaggctgagc agaatgaaga agatgagccc aaggggctgg
     1441 gtgggtccag ggaggccagg ggctcggtgg gtggcgcttc ctggtcacat ccacagtttg
     1501 agaaataata atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     1681 gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1741 ctgagcaata actatcataa cccctagggt atacccatct aattggaacc agataagtga
     1801 aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc
     1861 cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc
     1921 ctcccagttc ccaactattt tgtccgccca caaccggttg acttgggtca actgtcagac
     1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattgactt
     2821 gggtcgctct tcctgtggat gcgcagatgc cctgcgtaag cgggtgtggg cggacaataa
     2881 agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa
     2941 tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat
     3001 ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg
     3061 tggccaaggg catgtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg
     3121 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
     3181 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
     3241 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
     3301 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
     3361 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
     3421 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
     3481 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
     3541 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac
     3601 atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct
     3661 gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     3721 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     3781 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     3841 gaccagttgc gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat
     3901 gtcaactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     3961 agcagcgaag tcgcc
//
