LOCUS       sequence-337990-        3987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full-length, human GIRK2 with C-terminal StrepTag II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Qiao P, Schrecke S, Lyu J, Zhu Y, Zhang T, Benavides A, Laganowsky A
  TITLE     Insight into the Phospholipid-Binding Preferences of Kir3.4.
  JOURNAL   Biochemistry. 2021 Dec 21;60(50):3813-3821. doi: 
            10.1021/acs.biochem.1c00615. Epub 2021 Nov 30.
  PUBMED    34846128
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.1c00615"; journalName: "Biochemistry"; date: 
            "2021-12-21- 21"; volume: "60"; issue: "50"; pages: "3813-3821"
COMMENT     Sequence Label: pACE-GIRK2-S
FEATURES             Location/Qualifiers
     source          1..3987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        111..202
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     127..144
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1494..1517
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    1544..1678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1581..1600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1635..1654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1799..1964
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2154..2742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2643..2662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  2877..3100
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3167..3700)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3889..3894,3895..3911,3912..3917))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag atcatggaga
      121 taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt
      181 tgtaataaaa aaacctataa ataaccgcca ccatggccaa gctgacagaa tccatgacta
      241 acgtcctgga gggcgactcc atggatcagg acgtcgaaag cccagtggcc attcaccagc
      301 caaagttgcc taagcaggcc agggatgacc tgccaagaca catcagccga gatcggacca
      361 aaaggaaaat ccagaggtac gtgaggaaag acggaaagtg caatgttcat cacggcaacg
      421 tgagggagac ctatcgctac ctgaccgata tcttcaccac attagtggac ctgaagtgga
      481 gattcaacct attgattttt gtcatggttt acacagtgac ctggctcttt tttggaatga
      541 tctggtggtt gatcgcatac atacggggag acatggacca catagaggac ccctcctgga
      601 ctccttgtgt taccaacctc aacgggttcg tctctgcttt tttattctca atagagacag
      661 aaaccaccat tggttatggc taccgggtca tcacagataa atgcccggag ggaattattc
      721 ttctcttaat ccaatctgtg ttggggtcca ttgtcaatgc attcatggtg ggatgcatgt
      781 ttgtaaaaat ctctcaaccc aagaagaggg cagagaccct ggtcttttcc acccatgcag
      841 tgatctccat gcgggatggg aaactgtgcc tgatgttccg ggtaggggac cttaggaatt
      901 cccacattgt ggaggcttcc atcagagcca agttgatcaa atccaaacag acctcggagg
      961 gggagttcat cccgttgaac cagacggata tcaacgtagg gtattacacg ggggatgacc
     1021 gtctgtttct ggtgtcaccg ctgatcatta gccatgaaat taaccaacag agtcctttct
     1081 gggagatctc caaagcccag ctgcccaaag aggaactgga aattgtggtc atcctagaag
     1141 gaatggtgga agccacaggg atgacatgcc aagctcgaag ctcctacatc accagtgaga
     1201 tcctgtgggg ttaccggttc acacctgtcc tgaccctgga ggacgggttc tacgaagttg
     1261 actacaacag cttccatgag acctatgaga ccagcacccc atcccttagt gccaaagagc
     1321 tggccgagtt agccagcagg gcagagctgc ccctgagttg gtctgtatcc agcaaactca
     1381 accaacatgc agaactggag actgaagagg aagaaaagaa cctcgaagag caaacagaaa
     1441 gaaatggtga tgtggcaaac ctggagaatg aatccaaagt tggtggcgct tcctggtcac
     1501 atccacagtt tgagaaataa taatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     1681 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     1741 ctgctgccac cgctgagcaa taactatcat aacccctagg gtatacccat ctaattggaa
     1801 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
     1861 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
     1921 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacaaccggt tgacttgggt
     1981 caactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2041 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2101 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2161 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2221 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2281 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2341 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2401 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2461 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2521 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2581 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2641 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2701 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2761 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2821 cctgattgac ttgggtcgct cttcctgtgg atgcgcagat gccctgcgta agcgggtgtg
     2881 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     2941 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     3001 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     3061 taaagagggg cgtggccaag ggcatgtaaa gactatattc gcggcgttgt gacaatttac
     3121 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     3181 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     3241 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     3301 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     3361 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     3421 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     3481 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     3541 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     3601 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     3661 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     3721 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     3781 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     3841 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     3901 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     3961 gcaaccttgg gcagcagcga agtcgcc
//
