LOCUS       sequence-338003-       12207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Eukaryotic expression of human SCN1A variant 1 isoform. This channel
            has been modified to be stably maintained in standard bacterial 
            strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   DeKeyser JM, Thompson CH, George AL Jr
  TITLE     Cryptic prokaryotic promoters explain instability of recombinant 
            neuronal sodium channels in bacteria.
  JOURNAL   J Biol Chem. 2021 Jan-Jun;296:100298. doi: 
            10.1016/j.jbc.2021.100298. Epub 2021 Jan 15.
  PUBMED    33460646
REFERENCE   2  (bases 1 to 12207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 
            2021 Jan 15."
COMMENT     Sequence Label: pIR-CMV-SCN1A-Variant-1-IRES-mScarlet
FEATURES             Location/Qualifiers
     source          1..12207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          3555..3687
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3670..3689
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(6834..7407,7408..7410,7411..7419)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7001..7018)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7228..7247
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7420..8115
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(7553..7571)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7821..7840
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8390..8511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8427..8446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8481..8500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8518..8973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8655..8676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8867..8886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9000..9104
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9106..9297
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9129..9149)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             9444..10238
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9444..10238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9498..9517)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10108..10127
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10426..10445)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10470..10517
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     repeat_region   10747..10809
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      11117..11705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11606..11625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   11835..11869
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      601 gcggccgcat ggagcaaaca gtgcttgtac caccaggacc tgacagcttc aacttcttca
      661 ccagagaatc tcttgcggct attgaaagac gcattgcaga agaaaaggca aagaatccca
      721 aaccagacaa aaaagatgac gacgaaaatg gcccaaagcc aaatagtgac ttggaagctg
      781 gaaagaacct tccatttatt tatggagaca ttcctccaga gatggtgtca gagcccctgg
      841 aggacctgga cccctactat atcaataaga aaacttttat agtattgaat aaagggaagg
      901 ccatcttccg gttcagtgcc acctctgccc tgtacatttt aactcccttc aatcctctta
      961 ggaaaatagc tattaagatt ttggtacatt cattattcag catgctaatt atgtgcacta
     1021 ttttgacaaa ctgtgtgttt atgacaatga gtaaccctcc tgattggaca aagaatgtag
     1081 aatacacctt cacaggaata tatacttttg aatcacttat aaaaattatt gcaaggggat
     1141 tctgtttaga agattttact ttccttcggg atccatggaa ctggctcgat ttcactgtca
     1201 ttacatttgc gtacgtcaca gagtttgtgg acctgggcaa tgtctcggca ttgagaacat
     1261 tcagagttct ccgagcattg aagacgattt cagtcattcc aggcctgaaa accattgtgg
     1321 gagccctgat ccagtctgtg aagaagctct cagatgtaat gatcctgact gtgttctgtc
     1381 tgagcgtatt tgctctaatt gggctgcagc tgttcatggg caacctgagg aataaatgta
     1441 tacaatggcc tcccaccaat gcttccttgg aggaacatag tatagaaaag aatataactg
     1501 tgaactacaa cggtacactt ataaatgaaa ctgtctttga gtttgactgg aagtcatata
     1561 ttcaagattc aagatatcat tatttcctgg agggtttttt agatgcacta ctatgtggaa
     1621 atagctctga tgcaggccaa tgtccagagg gatatatgtg tgtgaaagct ggtagaaatc
     1681 ccaattatgg ctacacaagc tttgatacct tcagttgggc ttttttgtcc ttgtttcgac
     1741 taatgactca ggacttctgg gaaaatcttt atcaactgac attacgtgct gctgggaaaa
     1801 cgtacatgat attcttcgta ttggtcattt tcttgggctc attctaccta ataaatttga
     1861 tcctggctgt ggtggccatg gcctacgagg aacagaatca ggccaccttg gaagaagcag
     1921 aacagaaaga ggccgaattt cagcagatga ttgaacagct taaaaagcaa caggaggcag
     1981 ctcagcaggc agcaacggca actgcctcag aacattccag agagcccagt gcagcaggca
     2041 ggctctcaga cagctcatct gaagcctcta agttgagttc caagagtgct aaggaaagaa
     2101 gaaatcggag gaagaaaaga aaacagaaag agcagtctgg tggggaagag aaagatgagg
     2161 atgaattcca aaaatctgaa tctgaggaca gcatcaggag gaaaggtttt cgcttctcca
     2221 ttgaagggaa ccgattgaca tatgaaaaga ggtactcctc cccacaccag tctttgttga
     2281 gcatccgtgg ctccctattt tcaccaaggc gaaatagcag aacaagcctt ttcagcttta
     2341 gagggcgagc aaaggatgtg ggatctgaga acgacttcgc agatgatgag cacagcacct
     2401 ttgaggataa cgagagccgt agagattcct tgtttgtgcc ccgacgacac ggagagagac
     2461 gcaacagcaa cctgagtcag accagtaggt catcccggat gctggcagtg tttccagcga
     2521 atgggaagat gcacagcact gtggattgca atggtgtggt ttccttggtt ggtggacctt
     2581 cagttcctac atcgcctgtt ggacagcttc tgccagaggt gataatagat aagccagcta
     2641 ctgatgacaa tggaacaacc actgaaactg aaatgagaaa gagaaggtca agttctttcc
     2701 acgtttccat ggactttcta gaagatcctt cccaaaggca acgagcaatg agtatagcca
     2761 gcattctaac aaatacagta gaagaacttg aagaatccag gcagaaatgc ccaccctgtt
     2821 ggtataaatt ttccaacata ttcttaatct gggactgttc tccatattgg ttaaaagtga
     2881 aacatgttgt caacctggtt gtgatggacc catttgttga cctggccatc accatctgta
     2941 ttgtcttaaa tactcttttc atggccatgg agcactatcc aatgacggac catttcaata
     3001 atgtgcttac agtaggaaac ttggttttca ctgggatctt tacagcagaa atgtttctga
     3061 aaattattgc catggatcct tactattatt tccaagaagg ctggaatatc tttgacggtt
     3121 ttattgtgac gcttagcctg gtagaacttg gactcgccaa tgtggaagga ttatctgttc
     3181 tccgttcatt tcgattgctg cgagttttca agttggcaaa atcttggcca acgttaaata
     3241 tgctaataaa gatcatcggc aattccgtgg gggctctggg aaatttaacc ctcgtcttgg
     3301 ccatcatcgt cttcattttt gccgtggtcg gcatgcagct ctttggtaaa agctacaaag
     3361 attgtgtctg caagatcgcc agtgattgtc aactcccacg ctggcacatg aatgacttct
     3421 tccactcctt cctgattgtg ttccgcgtgc tgtgtgggga gtggatagag accatgtggg
     3481 actgtatgga ggttgctggt caagccatgt gccttactgt cttcatgatg gtcatggtga
     3541 ttggaaacct agtggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac
     3601 tgggcttgtc gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg
     3661 acatccactt tgcctttctc tccacaggtc ctgaatctct ttctggcctt gcttctgagc
     3721 tcatttagtg cagacaacct tgcagccact gatgatgata atgaaatgaa taatctccaa
     3781 attgctgtgg ataggatgca caaaggagta gcttatgtga aaagaaaaat atatgaattt
     3841 attcaacagt ccttcattag gaaacaaaag attttagatg aaattaaacc acttgatgat
     3901 ctaaacaaca agaaagacag ttgtatgtcc aatcatacag cagaaattgg gaaagatctt
     3961 gactatctta aagatgtaaa tggaactaca agtggtatag gaactggcag cagtgttgaa
     4021 aaatacatta ttgatgaaag tgattacatg tcattcataa acaaccccag tcttactgtg
     4081 actgtaccaa ttgctgtagg agaatctgac tttgaaaatt taaacacgga agactttagt
     4141 agtgaatcgg atctggaaga aagcaaagag aaactgaatg aaagcagtag ctcatcagaa
     4201 ggtagcactg tggacatcgg cgcacctgta gaagaacagc ccgtagtgga acctgaagaa
     4261 actcttgaac cagaagcttg tttcactgaa ggctgtgtac aaagattcaa gtgttgtcaa
     4321 atcaatgtgg aagaaggcag aggaaaacaa tggtggaacc tgagaaggac gtgtttccga
     4381 atagttgaac ataactggtt tgagaccttc attgttttca tgattctcct tagtagtggt
     4441 gctctggcat ttgaagatat atatattgat cagcgaaaga cgattaagac gatgttggaa
     4501 tatgctgaca aggttttcac ttacattttc attctggaaa tgcttctaaa atgggtggca
     4561 tatggctatc aaacatattt caccaatgcc tggtgttggc tggacttctt aattgttgat
     4621 gtttcattgg tcagtttaac agcaaatgcc ttgggttact cagaacttgg agccatcaaa
     4681 tctctcagga cactaagagc tctgagacct ctaagagcct tatctcgatt tgaagggatg
     4741 agggtggttg tgaatgccct tttaggagca attccatcca tcatgaatgt gcttctggtt
     4801 tgtcttatat tctggctaat tttcagcatc atgggcgtaa atttgtttgc tggcaaattc
     4861 taccactgta ttaacaccac aactggtgac aggttcgaca tcgaagacgt gaataatcat
     4921 actgattgcc taaaactaat agaaagaaat gagactgctc gatggaaaaa tgtgaaagta
     4981 aactttgata atgtaggatt tgggtatctc tctttgcttc aagttgccac attcaaagga
     5041 tggatggata taatgtatgc agcagttgat tccagaaatg tggaactcca gcctaagtat
     5101 gaagaaagtc tgtacatgta tctttacttt gttattttca tcatctttgg gtccttcttc
     5161 accttgaacc tgtttattgg tgtcatcata gataatttca accagcagaa aaagaagttt
     5221 ggaggtcaag acatctttat gacagaagaa cagaagaaat actataatgc aatgaaaaaa
     5281 ttaggatcga aaaaaccgca aaagcctata cctcgaccag gaaacaaatt tcaaggaatg
     5341 gtctttgact tcgtaaccag acaagttttt gacataagca tcatgattct catctgtctt
     5401 aacatggtca caatgatggt ggaaacagat gaccagagtg aatatgtgac taccattttg
     5461 tcacgcatca atctggtgtt cattgtgcta tttactggag agtgtgtact gaaactcatc
     5521 tctctacgcc attattattt taccattgga tggaatattt ttgattttgt ggttgtcatt
     5581 ctctccattg taggtatgtt tcttgccgag ctgatagaaa agtatttcgt gtcccctacc
     5641 ctgttccgag tgatccgtct tgctaggatt ggccgaatcc tacgtctgat caaaggagca
     5701 aaggggatcc gcacgctgct ctttgctttg atgatgtccc ttcctgcgtt gtttaacatc
     5761 ggcctcctac tcttcctagt catgttcatc tacgccatct ttgggatgtc caactttgcc
     5821 tatgttaaga gggaagttgg gatcgatgac atgttcaact ttgagacctt tggcaacagc
     5881 atgatctgcc tattccaaat tacaacctct gctggctggg atggattgct agcacccatt
     5941 ctcaacagta agccacccga ctgtgaccct aataaagtta accctggaag ctcagttaag
     6001 ggagactgtg ggaacccatc tgttggaatt ttcttttttg tcagttacat catcatatcc
     6061 ttcctggttg tggtgaacat gtacatcgcg gtcatcctgg agaacttcag tgttgctact
     6121 gaagaaagtg cagagcctct gagtgaggat gactttgaga tgttctatga ggtttgggag
     6181 aagtttgatc ccgatgcaac tcagttcatg gaatttgaaa aattatctca gtttgcagct
     6241 gcgcttgaac cgcctctcaa tctgccacaa ccaaacaaac tccagctcat tgccatggat
     6301 ttgcccatgg tgagtggtga ccggatccac tgtcttgata tcttatttgc ttttacaaag
     6361 cgggttctag gagagagtgg agagatggat gctctacgaa tacagatgga agagcgattc
     6421 atggcttcca atccttccaa ggtctcctat cagccaatca ctactacttt aaaacgaaaa
     6481 caagaggaag tatctgctgt cattattcag cgtgcttaca gacgccacct tttaaagcga
     6541 actgtaaaac aagcttcctt tacgtacaat aaaaacaaaa tcaaaggtgg ggctaatctt
     6601 cttataaaag aagacatgat aattgacaga ataaatgaaa actctattac agaaaaaact
     6661 gatctgacca tgtccactgc agcttgtcca ccttcctatg accgggtgac aaagccaatt
     6721 gtggaaaaac atgagcaaga aggcaaagat gaaaaagcca aagggaaata acgatcgcct
     6781 gcaggagtca agggcgaatt cgtttatcag ggaaagtaaa aagtaaggat ccgcccctct
     6841 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6901 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6961 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     7021 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7081 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     7141 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     7201 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     7261 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     7321 tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     7381 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggca
     7441 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
     7501 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7561 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
     7621 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc
     7681 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc
     7741 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
     7801 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
     7861 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
     7921 ctgaaggacg gaggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
     7981 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag
     8041 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
     8101 gacgagctgt acaagtaacc tgcaggaatt ccccttccca acagttgcgc agcctgaatg
     8161 gcgaatggag atccaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     8221 tgtattttag attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt
     8281 catgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     8341 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     8401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     8521 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8581 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8641 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8701 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8761 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8821 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8881 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8941 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     9001 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9061 aataaccctg ataaatgctt caataatatt gaaaaaggaa gaatcctgag gcggaaagaa
     9121 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     9181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9301 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9361 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     9421 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     9481 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     9541 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     9601 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     9661 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     9721 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     9781 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     9841 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     9901 tcgatcagga tgatctggac gaagaacatc aggggctcgc gccagccgaa ctgttcgcca
     9961 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
    10021 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    10081 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagaacttg
    10141 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    10201 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
    10261 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    10321 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    10381 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
    10441 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    10501 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
    10561 cccactgcct aacgcgtttt tgttacttta tagaagaaat tttgagtttt tgtttttttt
    10621 taataaataa ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta
    10681 aatataataa aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat
    10741 aaaacacatg cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc
    10801 tttctagggt taaacgcgtt aggcatacgc atccaccgag accccattgg ggccaatacg
    10861 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
    10921 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
    10981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
    11821 agttattaat ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa
    11881 tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc
    11941 gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac
    12001 tataacgacc gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt
    12061 aactcatacg ataattatat tgttatttca tgttctactt acgtgataac ttattatata
    12121 tatattttct tgttatagat aatgcattag ttattaatag taatcaatta cggggtcatt
    12181 agttcatagc ccatatatgg agttccg
//
