LOCUS       sequence-338004-       10463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Murphy KC, Campellone KG
  TITLE     Lambda Red-mediated recombinogenic engineering of enterohemorrhagic 
            and enteropathogenic E. coli.
  JOURNAL   BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.1186/1471-2199-4-11.
  PUBMED    14672541
REFERENCE   2  (bases 1 to 10463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Biol."; date: "2003-12-13"; volume: "4:11. doi"; pages: " 
            10.1186/1471-2199-4-11"
COMMENT     Sequence Label: pTP223
FEATURES             Location/Qualifiers
     source          1..10463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     56..75
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(156..173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(327..915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(407..426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1545..1550,1551..1568,1569..1575)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1583..1599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1633..2049
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             2055..2840
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2837..3517
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     promoter        join(3900..3905,3906..3922,3923..3928)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             3976..5166
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(4328..4347)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        7823..7900
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7901..8983
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7920..7939)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(9032..9037,9038..9055,9056..9062)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    9070..9086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9094..9110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9094..9110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9106..9675
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNKGLKIDFFLYILHGI
                     TDQCVQLVRLFFKIKRSAGMGFLLRL"
     primer_bind     complement(9126..9143)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9126..9142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9135..9157)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             10171..10362
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(10356..10378)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
       61 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
      121 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aacggtgatc cccaccaatt
      961 cagaagaaga tgatggagcc acagtccctc cctgaacatc acgtatatag ttagcctgac
     1021 atccaacaag gaggtttatc gcgaatattc ccacaaaaaa tcttttcctc ataactcgat
     1081 ccttataaaa tgaaaagaat atatgacgag gtttaattta tgagcttaag atactacata
     1141 aaaaatattt tatttggcct gtactgcaca cttatatata tataccttat aacaaaaaac
     1201 agcgaagagt attatttcct tgtgacagat aagatgctat atgcaatagt gataagcact
     1261 attctatgtc catattcaaa atatgctatt gaacacatag cttttaactt cataaagaaa
     1321 gattttttcg aaagaagaaa aaatctaaat aacgcccccg tagcaaaatt aaacctattt
     1381 atgctatata atctattttg tttggtccta gcaatcccat ttggattgct aggacttttt
     1441 atatcaataa agaataatta aatccctaat acctcattta tcgtattaag tttattctta
     1501 tcaatatagg agcatagaat tctcactcat taggcacccc aggctttaca ctttatgctt
     1561 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagtcga
     1621 cgcttataaa aaatggatat taatactgaa actgagatca agcaaaagca ttcactaacc
     1681 ccctttcctg ttttcctaat cagcccggca tttcgcgggc gatattttca cagctatttc
     1741 aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg
     1801 cgtcactacc agcagctcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa
     1861 aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg
     1921 gccagcaaaa aatccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg
     1981 gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca
     2041 gaggtataaa acgaatgagt actgcactcg caacgctggc tgggaagctg gctgaacgtg
     2101 tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta
     2161 aaggtgatgc cagcgatgcg cagttcatcg cattactgat cgttgccaac cagtacggcc
     2221 ttaatccgtg gacgaaagaa atttacgcct ttcctgataa gcagaatggc atcgttccgg
     2281 tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gatggcatgg
     2341 actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc
     2401 cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa actcgcgaag
     2461 gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgt cataaagcca
     2521 tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg
     2581 agcgcattgt cgaaaatact gcatacactg cagaacgtca gccggaacgc gacatcactc
     2641 cggttaacga tgaaacaatg caggagatta acactctgct gatcgccctg gataaaacat
     2701 gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt
     2761 cagaactgac acaggccgaa gcagtaaaag ctcttggatt cctgaaacag aaagccgcag
     2821 agcagaaggt ggcagcatga caccggacat tatcctgcag cgtaccggga tcgatgtgag
     2881 agctgtcgaa cagggggaag atgcgtggca caaattacgg ctcggcgtca tcaccgcttc
     2941 agaagttcac aacgtgatag caaaaccccg ctccggaaag aagtggcctg acatgaaaat
     3001 gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg aagttaacgc
     3061 taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt ttgaattcac
     3121 ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta tgcgtaccgc
     3181 ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat gcccgtttac
     3241 ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt cagcttacat
     3301 ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat gcctggtact ttgccaacta
     3361 tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg atgaaaagta
     3421 catggcgagt tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg aggcactggc
     3481 tgaaattggt tttgtatttg gggagcaatg gcgatgacgc atcctcacga taatatccgg
     3541 gtaggcgcaa tcactttcgt ctctctaggg aattctaatt tttcgcttga gaacttggca
     3601 tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat ttccacagtt
     3661 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg
     3721 ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg
     3781 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc
     3841 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttctcatgtt
     3901 tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc
     3961 agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     4021 ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     4081 gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     4141 caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     4201 ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     4261 ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     4321 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     4381 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     4441 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     4501 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     4561 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     4621 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     4681 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     4741 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     4801 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     4861 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     4921 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     4981 cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc
     5041 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     5101 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     5161 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     5221 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     5281 cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac
     5341 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     5401 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     5461 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     5521 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     5581 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     5641 tttttctctg gtgccgccct atccctttgt gcagcttgcc acgctcaaag gggtttgagg
     5701 tccaaccgta cgaaaacgta cggtaagagg aaaattatcg tctgaaaaat cgattagtag
     5761 acaagaaagt ccgttaagtg ccaattttcg attaaaaaga caccgttttg atggcgtttt
     5821 ccaatgtaca ttatgtttcg atatatcaga cagttacttc actaacgtac gttttcgttc
     5881 tattggcctt cagaccccat atccttaatg tcctttattt gctggggtta tcagatcccc
     5941 ccgacacgtt taattaatgc tttctccgcc ggagatcgac gcacagcgtt ctgtgctcta
     6001 tgatgttatt tcttaataat catccaggta ttctctttat caccatacgt agtgcgagtg
     6061 tccaccttaa cgcagggctt tccgtcacag cgcgatatgt cagccagcgg ggctttcttt
     6121 tgccagaccg cttccatcct ctgcatttca gcaatctggc tatacccgtc attcataaac
     6181 cacgtaaatg ccgtcacgca ggaagccagg acgaagaata tcgtcagtac aagataaatc
     6241 gcggatttcc acgtatagcg tgacatctca cgacgcattt catggatcat cgctttcgcc
     6301 gtatcggcag cctgattcag cgcttctgtc gccggtttct gctgtgctaa tccggcttgt
     6361 ttcagttctt tctcaacctg agtgagcgcg gaactcaccg atttcctgac ggtgtcagtc
     6421 atattaccgg acgcgctgtc cagctcacga atgaccctgc tcagcgtttc actttgctgc
     6481 tgtaattgtg atgaggcggc ctgaaactgt tctgtcagag aagtaacacg cttttccagc
     6541 gcctgatgat gcccgataag ggcggcaatt tgtttaattt cgtcgctcat acaaaatcct
     6601 gcctatcgtg agaatgacca gcctttatcc ggcttctgtc gtatctgttc ggcgagtcgc
     6661 tgtcgttctt tctcctgctg acgctgtttt tccgccagac gttcgcgctc tctctgcctt
     6721 tccatctcct gatgtatccc ctggaactcc gccatcgcat cgttaacagc aggctggatg
     6781 tcagggtgtt gtattgccat ttcagtacct cacgactcgg acaaaatgtc gttgcgcgca
     6841 cagtacagcg caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt
     6901 ataggaaggg tgttttcggc tacaatcaaa acatgcccga atgtgcacca ggtgcaccac
     6961 gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa
     7021 accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc
     7081 ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga
     7141 ctgctggaga cgctgcagga agagggatat gtccgccgta gcccctccga tgatagtttt
     7201 cgactgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct
     7261 gcactggcgg ccccactgct gggcgatctg ttgcgcgaag tggtatggcc gacagatgtg
     7321 tccacgctgg atgttgatgc aatggtggta cgcgaaacca ctcaccgttt cagccgctta
     7381 tcctttcacc gggcaatggt cgggcgacgt ttgccgcttc tgaaaaccgc ctcgggcctg
     7441 acctggctgg ccttttgccc ggaacaagac cgcaaggaat taatcgaaat gttagcctcc
     7501 cgccccggtg atgactatca actggcacgg gaaccgttaa agctggaagc cattctggcg
     7561 cgcgcgcgca aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc
     7621 gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg
     7681 tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa
     7741 cgggtagcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggaagg
     7801 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     7861 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     7921 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     7981 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     8041 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     8101 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     8161 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     8221 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     8281 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     8341 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     8401 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     8461 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     8521 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     8581 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     8641 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     8701 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     8761 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     8821 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     8881 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9001 taagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     9061 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9121 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9181 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9241 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     9301 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     9361 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc
     9421 tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     9481 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     9541 ggcgttaaca agggactgaa gatcgatttc ttcctgtata tccttcatgg catcactgac
     9601 cagtgcgttc agcttgtcag gctctttttc aaaatcaaac gttctgccgg aatgggattc
     9661 ctgctcaggc tctgacttct tccgggttac cctgaagtac cgttgcacct tcccggttac
     9721 gctccctccc cagaaggtaa tcaaccggac cactgccacc accttttccc cgggcatgaa
     9781 atttaactat catcccgcgc cccctgttcc ctgacggcca gacgcagccg gcgcagctca
     9841 tccccgatgg ccatcagtgc ggccaccacc tgaacccggt cgccggaaga ccactgcccg
     9901 ctgttcacct tcctcgctgt ctgattcagg ttattcccga tggcggccag ctgacgcagt
     9961 aacggcggag ccaaagttgg cagcttcccg gaacgggcaa ccggctcacc caggcagacc
    10021 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
    10081 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
    10141 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
    10201 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
    10261 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
    10321 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10381 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10441 gcgggtgttg gcgggtgtcg ggg
//
