LOCUS       sequence-338006-       11315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  No promoter, C-terminal NLUC:3xFlag:10xHis tag, 
            attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP 
            backbone, SpcR for bacteria, BASTA R for plant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Urquiza-Garcia U, Millar AJ
  TITLE     Expanding the bioluminescent reporter toolkit for plant science with
            NanoLUC.
  JOURNAL   Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. 
            eCollection 2019.
  PUBMED    31316580
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2019-07-8"; volume: "15:68. doi"; pages: " 
            10.1186/s13007-019-0454-4. eCollection 2019"
COMMENT     Sequence Label: pGWB601NL3F10H
FEATURES             Location/Qualifiers
     source          1..11315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(70..92)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    81..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    150..171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(207..459)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(619..1170)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1222..1401)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1907..1931
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2452..3243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3489..4077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3978..3997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4231..4248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4263..4403
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4329..4348)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4489..4511
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4747..4941
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5007..6080)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6509..7138)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8439..8463
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8651..8673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8665..8682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8666..8682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8723..8847
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(8884..8889,8890..8907,8908..8914)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             8968..9627
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9034..9053)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             9969..10274
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10153..10172
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10315..10439)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             10450..10962
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             10972..11037
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11038..11067
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      join(11087..11315,1..24)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cggtgtcatc tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt
      181 aggcgcgcct atttctatta attcccgatc tagtaacata gatgacaccg cgcgcgataa
      241 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
      301 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
      361 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
      421 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggggac
      481 gtccccagct cggtaccccc tcctgggcac ggtcgtacgc gatccgccgc ggttccacct
      541 caccattgcg ggtatgccag ttgagttcga tgagttcccg taccagctcc aactcgggcg
      601 atgccacgcg cactccgttc agatctcggt gacgggcagg accggacggg gcggtaccgg
      661 caggctgaag tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc
      721 cgcccgcagc atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc
      781 gttgggcagc ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag
      841 caggtgggtg tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac
      901 ggtcgactcg gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat
      961 gccggcgacc tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac
     1021 gaggtcgtcc gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt
     1081 ctcgatgtag tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg
     1141 gatgtcggcc gggcgtcgtt ctgggctcat atcttattgc cccccgggat cagatctgga
     1201 ttgagagtga atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga
     1261 gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc
     1321 aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg
     1381 gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc
     1441 ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcttgatc ccctgcgcca
     1501 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     1561 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     1621 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     1681 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     1741 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     1801 agctgatccg tcgagattaa tagaaataaa ttcagcctaa ttcggcgtta attcagtaca
     1861 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     1921 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     1981 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     2041 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     2101 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     2161 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     2221 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2281 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     2341 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2401 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     2461 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     2521 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     2581 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     2641 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     2701 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     2761 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     2821 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2881 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2941 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     3001 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     3061 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     3121 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     3181 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     3241 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     3301 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     3361 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3601 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4261 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     4321 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     4381 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     4441 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4501 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
     4561 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
     4621 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
     4681 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
     4741 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
     4801 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
     4861 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
     4921 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
     4981 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
     5041 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
     5101 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
     5161 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
     5221 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
     5281 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
     5341 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
     5401 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
     5461 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
     5521 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
     5581 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
     5641 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
     5701 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
     5761 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
     5821 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
     5881 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
     5941 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
     6001 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
     6061 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     6121 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     6181 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     6241 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     6301 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
     6361 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
     6421 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
     6481 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
     6541 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
     6601 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
     6661 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
     6721 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
     6781 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
     6841 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
     6901 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
     6961 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
     7021 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
     7081 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
     7141 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
     7201 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
     7261 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
     7321 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
     7381 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
     7441 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
     7501 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
     7561 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
     7621 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
     7681 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
     7741 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
     7801 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
     7861 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
     7921 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
     7981 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
     8041 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
     8101 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
     8161 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
     8221 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
     8281 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
     8341 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
     8401 aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
     8461 tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg
     8521 ctctagcatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8581 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8641 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg
     8701 caggtcgact ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     8761 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     8821 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
     8881 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
     8941 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     9001 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     9061 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     9121 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     9181 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     9241 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     9301 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     9361 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     9421 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     9481 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     9541 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     9601 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     9661 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     9721 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     9781 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     9841 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     9901 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     9961 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
    10021 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
    10081 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
    10141 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
    10201 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
    10261 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
    10321 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
    10381 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
    10441 gataacagca tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc
    10501 tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg
    10561 gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac
    10621 atccatgtca tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa
    10681 atttttaagg tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc
    10741 acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa
    10801 ggcatcgccg tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac
    10861 aaaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc
    10921 aacggagtga ccggctggcg gctgtgcgaa cgcattctgg cgcctcgagt tgattacaag
    10981 gatcatgatg gtgattacaa ggatcacgac atcgactaca aggatgacga tgacaagcat
    11041 caccatcacc atcaccatca ccatcactga gagctcgaat ttccccgatc gttcaaacat
    11101 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
    11161 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
    11221 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
    11281 aatatagcgc gcaaactagg ataaattatc gcgcg
//
