LOCUS       sequence-338011-        7954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express drosophila SSRP1 in E.coli expression system. Generated from
            pDONR-SSRP1 by Gateway system. Used for custom antibody 
            purification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Tettey TT, Gao X, Shao W, Li H, Story BA, Chitsazan AD, Glaser RL, 
            Goode ZH, Seidel CW, Conaway RC, Zeitlinger J, Blanchette M, Conaway
            JW
  TITLE     A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing.
  JOURNAL   Cell Rep. 2019 Jun 25;27(13):3770-3779.e7. doi: 
            10.1016/j.celrep.2019.05.099.
  PUBMED    31242411
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.05"; journalName: "Cell Rep"; date: 
            "2019-06-25- 25"; volume: "27"; issue: "13"; pages: "3770-3779.e7"
COMMENT     Sequence Label: pET-DEST42 -SSRP1
FEATURES             Location/Qualifiers
     source          1..7954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2282..2306)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2326..2367
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2377..2394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2451..2469)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2465..2512
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(2687..2692,2693..2709,2710..2715))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2746..2764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2832..2936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2937..3005,3006..3797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3155..3174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3968..4556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4457..4476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4710..4727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4742..4881
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4807..4826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4967..4989
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4983..5174)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6486..7568)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7530..7549
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7569..7646)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7852..7871
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggaa tatcacaagt ttgtacaaaa aagcaggctt aatgacagac
      121 tctctggagt acaacgacat aaacgccgaa gtgcgcggag tcttgtgttc cggacgccta
      181 aagatgaccg agcagaacat catcttcaag aacaccaaga ccggcaaggt ggagcagatc
      241 tcggcagagg acatagacct gatcaattcg cagaagttcg tgggcacctg gggactgagg
      301 gtgttcacca aaggcggcgt gctccaccgc ttcaccggat tccgcgacag cgagcacgag
      361 aagctgggca agtttatcaa ggctgcctac tcgcaggaga tggtcgagaa ggagatgtgc
      421 gtcaagggct ggaactgggg caccgcccgc ttcatgggct ccgtcctgag cttcgacaag
      481 gagtcgaaga ccatcttcga ggtgccgctg tcgcacgttt cgcagtgcgt gaccggcaag
      541 aacgaggtca ccctggagtt ccaccaaaac gacgatgcgc ccgtgggtct actggagatg
      601 cggttccaca tacccgccgt ggagtcggcc gaggaggatc cggtagacaa gttccaccag
      661 aacgtaatga gcaaggcctc ggtcatctcg gcttcgggcg agtccatcgc cattttcaga
      721 gagatccaga tcctcacgcc tcgcggtcgc tatgacatca agatcttctc gaccttcttc
      781 cagctgcacg gcaagacgtt cgactacaag attcccatgg actcggtgct gcggctcttc
      841 atgctgcccc acaaagacag tcgacagatg ttctttgtgc tctccttgga tccgcccatc
      901 aagcagggac aaacgcgtta ccactacctg gtcctgctgt ttgctcccga tgaggagacc
      961 accattgagc tgccattctc ggaagccgag ttgcgagaca agtacgaggg caagctggag
     1021 aaagagatct ccgggccggt gtacgaggtg atgggcaaag tgatgaaggt gctgatcggt
     1081 cgaaaaatta ccggacccgg taactttatc ggacactctg gcacggctgc agtgggctgc
     1141 tcgttcaagg ctgcagctgg atatctgtat cccctggagc gaggattcat ctatatccac
     1201 aagccaccgc tgcatatccg ctttgaggag attagttctg tgaactttgc ccgcagcggc
     1261 ggatccacgc gatctttcga cttcgaagtg acgctcaaga acggaactgt tcacatcttc
     1321 tcctccatcg agaaggagga gtatgccaag ctcttcgact acatcacaca gaagaagttg
     1381 catgtcagca acatgggcaa ggacaagagc ggctacaagg acgtggactt tggtgattcg
     1441 gacaacgaga acgaaccaga tgcctatctg gctcgcctca aggctgaggc gagggaaaag
     1501 gaggaggacg acgacgatgg cgactcggat gaagagtcca cggatgagga cttcaagccc
     1561 aacgagaacg agtccgatgt ggccgaggag tatgacagca acgtggagag tgattcggac
     1621 gatgacagcg atgctagtgg cggcggaggc gacagcgacg gcgccaagaa aaagaaggag
     1681 aagaagtccg agaagaaaga gaaaaaggag aaaaaacaca aggagaagga gagaacaaag
     1741 aaaccctcca agaagaagaa ggactctggc aaacccaagc gcgccaccac cgctttcatg
     1801 ctctggctga acgacacgcg cgagagcatc aagagggaaa atccgggcat aaaggttacc
     1861 gagatcgcca agaagggcgg cgagatgtgg aaggagctga aggacaagtc caagtgggag
     1921 gatgcggcgg ccaaggacaa gcagcgctac cacgacgaga tgcgcaacta caagcctgaa
     1981 gcgggcggtg acagcgacaa cgagaagggt ggaaagtcct ccaagaagcg caagacagag
     2041 ccttctccat ccaagaaggc gaatacctcg ggtagcggct tcaagagcaa ggagtacatt
     2101 tcggacgacg actccaccag ctccgacgac gagaaggaca acgagcctgc cacgaagaag
     2161 agcaagccgc catccgacgg cgatgccaag aagaaaaagg ccaagagcga gagcgaaccg
     2221 gaggagagcg aggaggacag caatgccagc gatgaggatg aggaagatga ggccagtgat
     2281 tacccagctt tcttgtacaa agtggtgatc aattcgaagc ttgaaggtaa gcctatccct
     2341 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     2401 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     2461 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     2521 aggaactata tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
     2581 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
     2641 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg
     2701 atgataagct gtcaaacatg agaattaatt cttgaagacg aaagggcctc gtgatacgcc
     2761 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2821 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2881 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2941 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3001 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3061 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3121 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     3181 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3241 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3301 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3361 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3421 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3481 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3541 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3601 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3661 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3721 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3781 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3841 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3901 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3961 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4021 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4081 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4141 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4201 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4261 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4321 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4381 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4441 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4501 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4561 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4621 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4681 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4741 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     4801 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     4861 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4921 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4981 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     5041 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     5101 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     5161 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     5221 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     5281 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     5341 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     5401 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     5461 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     5521 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     5581 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     5641 ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     5701 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     5761 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg
     5821 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
     5881 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
     5941 cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
     6001 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     6061 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     6121 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc
     6181 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
     6241 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
     6301 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
     6361 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     6421 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     6481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
     6601 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
     6661 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
     6721 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg
     6781 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
     6841 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
     6901 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
     6961 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
     7021 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
     7081 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
     7141 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
     7201 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
     7261 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
     7321 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
     7381 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
     7441 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
     7501 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
     7561 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
     7621 aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg
     7681 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     7741 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     7801 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     7861 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     7921 ggcgtagagg atcgagatct cgatcccgcg aaat
//
