LOCUS       sequence-338038-        7764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon
            with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' 
            PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa
            DM, Boeke JD, Noyes MB
  TITLE     Engineered dual selection for directed evolution of SpCas9 PAM 
            specificity.
  JOURNAL   Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x.
  PUBMED    33441553
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-13"; volume: "12(1):349. doi"; pages: " 
            10.1038/s41467-020-20650-x"
COMMENT     Sequence Label: pGG198
FEATURES             Location/Qualifiers
     source          1..7764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..98
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(636..1586)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1634..1856
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2348..2442)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2473..3267)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2584..2603)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3194..3213
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      complement(3416..3443)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(3459..3530)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3545..4255)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3823..3842)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4092..4110
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             4261..4269
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4322..5125)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      5231..5317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5409..5436
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     5514..5532
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5630..5635,5636..5653,5654..5660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     5712..5728
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5724..6386
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     RBS             6434..6442
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6448..7164
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6448..7161
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7179..7250
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7266..7293
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7308..7763)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7445..7466)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7657..7676
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctagggta
      121 cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca
      181 gatccggctt cagccggttt gccggctgaa agcgctattt cttccagaat tgccatgatt
      241 ttttccccac gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca
      301 gcatcgcctg tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt
      361 gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta
      421 catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa
      481 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
      541 acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg
      601 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
      661 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
      721 tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
      781 gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg
      841 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
      901 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
      961 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     1021 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     1081 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     1141 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     1201 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     1261 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     1321 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     1381 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     1441 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     1501 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     1561 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     1621 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     1681 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     1741 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     1801 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     1861 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     1921 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     1981 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     2041 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     2101 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     2161 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     2221 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     2281 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag
     2341 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     2401 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctctcgaacc
     2461 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     2521 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     2581 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     2641 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     2701 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     2761 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     2821 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     2881 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     2941 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     3001 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     3061 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     3121 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     3181 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     3241 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
     3301 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     3361 gggcttccca accttaccag agggcgcccc agctggcaat tccgacgtct ataaacgcag
     3421 aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac cgacaaacaa
     3481 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggct
     3541 atcattactt gtacagctcg tccatgcctc ctgtggagtg gcggccctcg gcgcgttcgt
     3601 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
     3661 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
     3721 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
     3781 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg
     3841 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
     3901 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
     3961 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
     4021 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
     4081 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
     4141 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
     4201 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatagctg
     4261 acctccttat taaagttaaa caaaacgccg gtgctgcagc tttaaataat cggtgtcact
     4321 attagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt
     4381 caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt
     4441 cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct
     4501 tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac
     4561 ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa
     4621 cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca
     4681 tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa
     4741 tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata
     4801 cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga
     4861 agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg
     4921 aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt
     4981 caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt
     5041 gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac
     5101 gttccttata tgtagctttc gacatagctg tttcctgtgt ggctaccagt agcgtattac
     5161 ttgaggaaca taagccgaaa ggccatagga tgtaaaaccg gtcgagcgag tgctttggcg
     5221 cgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     5281 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     5341 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     5401 aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt
     5461 tatttttcta aatacattca aatatgtatc cgctcatgac attaacctat aaaaataggc
     5521 gtatcacgag gccctttcgt cttcaaacgc gtgtacaaaa ccggcggccg caacacgctg
     5581 aacttgtggc cgtttacgtc gctgtccagc tcgaccagga tgggaattct ttacacttta
     5641 tgcttccggc tcgtatgttg tgtcgaccgc ccatcctggt cgagctggac ggcgacgata
     5701 acaatttcac acaggaaaca gctatgacag agcagaaagc cctagtaaag cgtattacaa
     5761 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
     5821 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
     5881 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
     5941 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
     6001 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg
     6061 gagtaaaaag gtttggatca ggatttgcgc ctttggatga ggcactttcc agagcggtgg
     6121 tagatctttc gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag
     6181 gagatctctc ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag gctagcagaa
     6241 ttaccctcca cgttgattgt ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt
     6301 tcaaggctct tgcggttgcc ataagagaag ccacctcgcc caatggtacc aacgatgttc
     6361 cctccaccaa aggtgttctt atgtagtgac accgattatt taaagctgca gggatccttt
     6421 tgtttaactt taataaggag gtcagctatg tctaaaggtg aagaattatt cactggtgtt
     6481 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
     6541 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt
     6601 aaattgccag ttccatggcc aaccttagtc actactttaa cttatggtgt tcaatgtttt
     6661 tcaagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
     6721 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
     6781 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     6841 gaagatggta acattttagg tcacaaattg gaatacaact ataactctca caatgtttac
     6901 atcatggctg acaaacaaaa gaatggtatc aaagttaact tcaaaattag acacaacatt
     6961 gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     7021 ccagtcttgt taccagacaa ccattactta tccactcaat ctgccttatc caaagatcca
     7081 aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg tattacccat
     7141 ggtatggatg aattgtacaa ataatagtga ccaggcatca aataaaacga aaggctcagt
     7201 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     7261 acactggctc accttcgggt gggcctttct gcgtttatac tcgagctaaa ttgtaagcgt
     7321 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7381 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7441 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7501 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7561 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7621 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7681 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7741 taatgcgccg ctacagggcg cgtc
//
