LOCUS       sequence-338045-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Covalently circularized nanodisc NW11 for studying membrane proteins
            and viral entry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, 
            Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G
  TITLE     Covalently circularized nanodiscs for studying membrane proteins and
            viral entry.
  JOURNAL   Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 
            2016 Nov 21.
  PUBMED    27869813
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4079"; journalName: "Nat Methods"; date: "2017-01"; 
            volume: "14"; issue: "1"; pages: "49-52"
COMMENT     Sequence Label: NW11
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             692..709
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      777..824
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cntcnnnnnn nnnttttgtt nnnctttang aaggagatat accatgggca gcagccatca
       61 tcatcatcat catgaaaacc tgtattttca gggcagcacc tttagcaaac tgcgtgaaca
      121 gctgggcccg gtgacccagg aattttggga taacctggaa aaagaaaccg aaggcctgcg
      181 tcaggaaatg agcaaagatc tggaagaggt gaaagcgaaa gtgcagccgt atctggatga
      241 ctttcagaaa aaatggcagg aagagatgga actgtatcgt cagaaagtgg aaccgctgcg
      301 tgcggaactg caggaaggcg cgcgtcagaa actgcatgaa ctgcaggaaa aactgagccc
      361 gctgggcgaa gagatgcgtg atcgtgcgcg tgcgcatgtg gatgcgctgc gtacccatct
      421 ggcgccgtat agcgatgaac tgcgtcagcg tctggcggcc cgtctggaag cgctgaaaga
      481 aaacggcggt gcgcgtctgg cggaatatca tgcgaaagcg accgaacatc tgagcacnnn
      541 gagcgaaaaa gcgaaaccgg cgctggaaga tctgcgtcag ggcctgctgc cggtgctgga
      601 aagctttaaa gtgagctttc tgagcgcgct ggaagagtat accaaaaaac tgaacaccca
      661 gctgccgggc accggcgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      721 tgctaancaa agcccgaaag gaagctgagt tggctgctgc caccgctgan caataactag
      781 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      841 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      901 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccg
//
