LOCUS       sequence-338046-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Covalently circularized nanodisc NW11 for studying membrane proteins
            and viral entry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, 
            Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G
  TITLE     Covalently circularized nanodiscs for studying membrane proteins and
            viral entry.
  JOURNAL   Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 
            2016 Nov 21.
  PUBMED    27869813
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4079"; journalName: "Nat Methods"; date: "2017-01"; 
            volume: "14"; issue: "1"; pages: "49-52"
COMMENT     Sequence Label: NW11
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(647..667)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(668..685)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             704..726
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    741..765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(765..784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(766..784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(868..887)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcnnncnnnc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcgccggt gcccggcagc tgggtgttca gttttttggt atactcttcc agcgcgctca
      121 gaaagctcac tttaaagctt tccagcaccg gcagcaggcc ctgacgcaga tcttccagcg
      181 ccggtttcgc tttttcgctc agggtgctca gatgttcggt cgctttcgca tgatattccg
      241 ccagacgcgc accgccgttt tctttcagcg cttccagacg ggccgccaga cgctgacgca
      301 gttcatcgct atacggcgcc agatgggtac gcagcgcatc cacatgcgca cgcgcacgat
      361 cacgcatctc ttcgcccagc gggctcagtt tttcctgcag ttcatgcagt ttctgacgcg
      421 cgccttcctg cagttccgca cgcagcggtt ccactttctg acgatacagt tccatctctt
      481 cctgccattt tttctgaaag tcatccagat acggctgcac tttcgctttc acctcttcca
      541 gatctttgct catttcctga cgcaggcctt cggtttcttt ttccaggtta tcccaaaatt
      601 cctgggtcac cgggcccagc tgttcacgca gtttgctaaa ggtgctgccc tgaaaataca
      661 ggttttcatg atgatgatga tgatggctgc tgcccatggt atatctcctt cttaaagtta
      721 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      781 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      841 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
      901 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gc
//
