LOCUS       sequence-33805-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD
  TITLE     TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral 
            sclerosis-linked mutations accelerate aggregation and increase 
            toxicity.
  JOURNAL   J Biol Chem. 2009 Jul 24. 284(30):20329-39.
  PUBMED    19465477
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2009 Jul 24. 284(30):20329-39."
COMMENT     Sequence Label: pDuet TDP-43 Q331K
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    168..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(192..211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(193..211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agctgcgctg tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct
      121 gccatatgta tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc
      181 gctcacaatt cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg
      241 attactttct gttcgactta agcattatgc ggccgcaagc ttctacattc cccagccaga
      301 agacttagaa tccatgcttg agccaaagcc tccattaaaa ccactgcccg accctgcatt
      361 ggatgctgat ccccaaccaa ttgctgcacc agaattagag ccactataag agttatttcc
      421 agaaccgaag gcctggtttg gctccctctg catgttgcct tggttttggt tattacccga
      481 tgggcctgac tggttctgct ggctggctaa catgcccatc ataccccaac tgctctttag
      541 tgctgcctgg gcggcagcca tcatggctgg attaatgctg aacgcaccaa agttcatccc
      601 accacccata ttactacctt gattgtttcc caaaccagct ccaccccctc tgctattacc
      661 aaatccaccc tgattcccaa agccacctgg attaccacca aatcttccac ttctttctaa
      721 ctgtctattg ctattgtgct taggttcggc attggatata tgaacgctga ttcctttaat
      781 gatcaagtcc tctccacaaa gagactgcgc aatctgatca tctgc
//
