LOCUS       sequence-33810-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Johnson BS, McCaffery JM, Lindquist S, Gitler AD
  TITLE     A yeast TDP-43 proteinopathy model: Exploring the molecular 
            determinants of TDP-43 aggregation and cellular toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Apr 29. 105(17):6439-44.
  PUBMED    18434538
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Apr 29. 105(17):6439-44."
COMMENT     Sequence Label: pRS303 Gal TDP-43 YFP
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..448
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    7..124
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     419..442
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     463..479
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     463..479
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    487..511
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctgtcgga ttagaagccg ccgagcgggt gacagccctc cgaaggaaga ctctcctccg
       61 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
      121 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
      181 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
      241 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
      301 tctattaaca gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
      361 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
      421 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
      481 cccatcacaa gtttgtacaa aaaagcaggc ttcgaaggag ataacaaaat gtctgaatat
      541 attcgggtaa ccgaagatga gaacgatgag cccattgaaa taccatcgga agacgctggg
      601 acggtgctgc tctccacggt tacagcccag tttccagggg cgtgtgggct tcgctacagg
      661 aatccagtgt ctcagtgtat gagaggtgtc cggctagtag aaggaattct gcatgcccca
      721 gatgctggct ggggaaatct ggtgtatgtt gtcaactatc c
//
