LOCUS       sequence-33811-e         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Johnson BS, McCaffery JM, Lindquist S, Gitler AD
  TITLE     A yeast TDP-43 proteinopathy model: Exploring the molecular 
            determinants of TDP-43 aggregation and cellular toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Apr 29. 105(17):6439-44.
  PUBMED    18434538
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Apr 29. 105(17):6439-44."
COMMENT     Sequence Label: pRS303 Gal TDP-43 YFP
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..279)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     419..438
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctggtaccg ggccccccct cgagttactt gtacagctcg tccatgccga gagtgatccc
       61 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      121 cagggcggac tggtagctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
      181 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
      241 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
      301 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      361 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      421 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      481 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcag
      541 gccgtagccg aaggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      601 gatgaacttc agggtcagct tgccgta
//
