LOCUS       sequence-33813-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Johnson BS, McCaffery JM, Lindquist S, Gitler AD
  TITLE     A yeast TDP-43 proteinopathy model: Exploring the molecular 
            determinants of TDP-43 aggregation and cellular toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Apr 29. 105(17):6439-44.
  PUBMED    18434538
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Apr 29. 105(17):6439-44."
COMMENT     Sequence Label: pDONR TDP43 WT YFP
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(106..357)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(148..169)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     497..516
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
       61 tataatgcca actttgtaca agaaagctgg gtcctagata gatctcttgt acagctcgtc
      121 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      181 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      241 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      301 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
      361 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
      421 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      481 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      541 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      601 gcgggcgaag cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg
      661 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgta
//
