LOCUS       sequence-338147-        9611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human BCL6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-BCL6
FEATURES             Location/Qualifiers
     source          1..9611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(45..63,64..86,87..105,106..128,129..147,148..170,
                     171..189,190..212,213..231,232..254,255..273,274..296,
                     297..315)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(87..105,106..128,129..147,148..170,171..189,190..212,
                     213..231,232..254,255..273,274..296,297..315,316..338,
                     339..357)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(129..147,148..170,171..189,190..212,213..231,232..254,
                     255..273,274..296,297..315,316..338,339..357,358..380,
                     381..399)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        432..470
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     467..491
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1734..2322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1787..1807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2205..2216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2325..2341)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2326..2342)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2847..3027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3053..3070)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3059..3283
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3329..3757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3416..3435)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3626..3647
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3766..3786)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3771..4100
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3951..4086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4013..4032
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4148..4195
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4214..4588
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4718..4839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4755..4774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4809..4828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4888..4904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4888..4904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4901..4923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4912..4928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4936..4942,4943..4960,4961..4966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4981..5002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5119..5136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5290..5878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5370..5389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6049..6840,6841..6909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6672..6691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6910..7014)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7089..7108)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7280..7659
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7661..7859
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7877..8057
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8104..8229
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8722..8955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9140..9184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9482..9599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agggacagca gagatccagt ttggttagat ctcgagttta ccactcccta tcagtgatag
       61 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      121 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      241 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      301 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      361 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcggt
      421 acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
      481 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
      541 accgatccag cctccgcggc cccgaattca ccggttctag agctagcatg gcctcgccgg
      601 ctgacagctg tatccagttc acccgccatg ccagtgatgt tcttctcaac cttaatcgtc
      661 tccggagtcg agacatcttg actgatgttg tcattgttgt gagccgtgag cagtttagag
      721 cccataaaac ggtcctcatg gcctgcagtg gcctgttcta tagcatcttt acagaccagt
      781 tgaaatgcaa ccttagtgtg atcaatctag atcctgagat caaccctgag ggattctgca
      841 tcctcctgga cttcatgtac acatctcggc tcaatttgcg ggagggcaac atcatggctg
      901 tgatggccac ggctatgtac ctgcagatgg agcatgttgt ggacacttgc cggaagttta
      961 ttaaggccag tgaagcagag atggtttctg ccatcaagcc tcctcgtgaa gagttcctca
     1021 acagccggat gctgatgccc caagacatca tggcctatcg gggtcgtgag gtggtggaga
     1081 acaacctgcc actgaggagc gcccctgggt gtgagagcag agcctttgcc cccagcctgt
     1141 acagtggcct gtccgcaccg ccagcctctt attccatgta cagccacctc cctgtcagca
     1201 gcctcctctt ctccgatgag gagtttcggg atgtccggat gcctgtggcc aaccccttcc
     1261 ccaaggagcg ggcactccca tgtgatagtg ccaggccagt ccctggtggg tacagccggc
     1321 cgactttgga ggtgtccccc aatgtgtgcc acagcaatat ctattcaccc aaggaaacaa
     1381 tcccagaaga ggcacgaagt gatatgcact acagtgtggc tgagggcctc aaacctgctg
     1441 ccccctcagc ccgaaatgcc ccctacttcc cttgtgacaa ggccagcaaa gaagaagaga
     1501 gaccctcctc ggaagatgag attgccatgc atttcgagcc ccccaatgca cccctgaacc
     1561 ggaagggtct ggttagtcca cagagccccc agaaatctga ctgccagccc aactcgccca
     1621 cagagtcctg cagcagtaag aatgcctgca tcctccaggc ttctggctcc cctccagcca
     1681 agagccccac tgaccccaaa gcctgctgat tcgaagatat caagcttatc gataatcaac
     1741 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     1801 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     1861 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     1921 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     1981 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2041 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2101 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2161 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2221 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2281 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     2341 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     2401 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     2461 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2521 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     2581 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     2641 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     2701 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     2761 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     2821 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2881 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2941 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3001 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     3061 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3121 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3181 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3241 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3301 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3361 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3421 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3481 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3541 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3601 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3661 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3721 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     3781 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3841 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3901 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4021 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4081 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4141 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4201 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     4261 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     4321 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     4381 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     4441 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     4501 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     4561 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     4621 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4681 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     4741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     4861 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5701 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5881 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5941 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6001 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6061 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6121 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6181 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6241 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6301 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6361 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6421 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6481 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6541 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6601 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6661 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6721 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6781 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6841 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6901 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6961 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7021 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     7081 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     7141 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     7201 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     7261 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     7321 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7381 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7441 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7501 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     7561 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     7621 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7681 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7741 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     7801 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     7861 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7921 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7981 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8041 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8101 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8161 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8221 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8281 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8341 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8401 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8461 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8521 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8581 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8641 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8701 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8761 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8821 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8881 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8941 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9001 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9061 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9121 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9181 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9241 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9301 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9361 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9421 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     9481 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9541 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9601 gggtttatta c
//
