LOCUS       sequence-33815-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     Birnbaum lab plasmids
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1 AKT3 HA (CT-gfp)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(2..709,710..712,713..715))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(39..59)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     654..682
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(770..796)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgtagagctc atccatgcca tgtgtaatcc cagcagcagt tacaaactca agaaggacca
       61 tgtggtcacg cttttcgttg ggatctttcg aaagggcaga ttgtgtcgac aggtaatggt
      121 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta
      181 gttgaacgga tccatcttca atgttgtggc gaattttgaa gttagctttg attccattct
      241 tttgtttgtc tgccgtgatg tatacattgt gtgagttata gttgtactcg agtttgtgtc
      301 cgagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgatacga ttaacaaggg
      361 tatcaccttc aaacttgact tcagcacgcg tcttgtagtt cccgtcatct ttgaaagata
      421 tagtgcgttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca
      481 tatgatccgg ataacgggaa aagcattgaa caccataaga gaaagtagtg acaagtgttg
      541 gccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagc tttccgtatg
      601 tagcatcacc ttcaccctct ccactgacag aaaatttgtg cccattaaca tcaccatcta
      661 attcaacaag aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag
      721 actcgagcgg ccgccactgt gctggatatc tgcagaattg cccttcctaa gcgtagtctg
      781 ggacgtcgta tgggtattcc cgtccgcttg cagagaagga gaactgaggg aagtgtggcc
      841 gccgctcgtt gtccatgccg tccatgccgt cgtcgt
//
