LOCUS       sequence-338151-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Groth AC, Fish M, Nusse R, Calos MP
  TITLE     Construction of transgenic Drosophila by using the site-specific 
            integrase from phage phiC31.
  JOURNAL   Genetics. 2004 Apr . 166(4):1775-82.
  PUBMED    15126397
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2004 Apr . 166(4):1775-82."
COMMENT     Sequence Label: pCaryP
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    125..224
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     misc_feature    join(326..880,881..911)
                     /label=P element 5' end
     rep_origin      complement(1146..1734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1226..1245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1905..2696,2697..2765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2528..2547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2766..2870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2938..2956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2994..3016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3116..3135
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(3673..3703,3704..3905)
                     /label=P element 3' end
                     /note="P element 3' end"
     CDS             7649..7660
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctcgagggc cctcgagggc cctcgagggc cctcgagggc cctcgagggg atccccctag
       61 tactgacgga cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg
      121 ttttcccagg tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt
      181 ctctcagttg ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca
      241 cgtacgcggg tgcttacgac cgtcagtcgc gcgagcgcga ctaggggcga gctcgaattg
      301 gcggccgccg gcggccgcca attcggctgc tgctctaaac gacgcatttc gtactccaaa
      361 gtacgaattt tttccctcaa gctcttattt tcattaaaca atgaacagga cctaacgcac
      421 agtcacgtta ttgtttacat aaatgatttt ttttactatt caaacttact ctgtttgtgt
      481 actcccactg gtatagcctt cttttatctt ttctggttca ggctctatca ctttactagg
      541 tacggcatct gcgttgagtc gcctcctttt aaatgtctga ccttttgcag gtgcagcctt
      601 ccactgcgaa tcattaaagt gggtatcaca aatttgggag ttttcaccaa ggctgcaccc
      661 aaggctctgc tcccacaatt ttctcttaat agcacacttc ggcacgtgaa ttaattttac
      721 tccagtcaca gctttgcagc aaaatttgca atatttcatt tttttttatt ccacgtaagg
      781 gttaatgttt tcaaaaaaaa attcgtccgc acacaacctt tcctctcaac aagcaaacgt
      841 gcactgaatt taagtgtata cttcggtaag cttcggctat cgacgggacc accttatgtt
      901 atttcatcat gggccagacc cacgtagtcc agcggcagat cggcggcgga gaagttaagc
      961 gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt gcagctaatt
     1021 tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc gtaacggaac
     1081 catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt caagaagaag
     1141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2881 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2941 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     3001 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     3061 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     3121 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca ccgaatcgcg
     3181 cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc
     3241 gggtgggaga cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag
     3301 agggcgcgcg atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga
     3361 taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc
     3421 caaagtctac ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt
     3481 gtttttattg gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca
     3541 cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact
     3601 ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga
     3661 gaatggccag accatgatga aataacataa ggtggtcccg tcggcaagag acatccactt
     3721 aacgtatgct tgcaataagt gcgagtgaaa ggaatagtat tctgagtgtc gtattgagtc
     3781 tgagtgagac agcgatatga ttgttgatta acccttagca tgtccgtggg gtttgaatta
     3841 actcataata ttaattagac gaaattattt ttaaagtttt atttttaata atttgcgagt
     3901 acgcaaagct tggctgcagg tcgactatta aatgattatc gcccgattac cacattgagt
     3961 ggtttaaaat agccataaaa tatgcaactg acgatggctt aagataaata cgtcgcagag
     4021 tcactcataa atttcgaacg cagcccgctg atttacctac ccctctaaac gattcatagt
     4081 atatgtacga gtatatccac taagcttttt cgagcactga ttttttcgct tgcacgagac
     4141 aagtgcacca ccgcaattgc aggcaaatta tgtctgaggt aatgattccg tttcgtgcaa
     4201 gattacacag aaatcaaatt acgacaacct ttattcagta agcaaacaaa gcctttgttg
     4261 gcatctaatt attccactta tggttgcgat ttcgggagct acaatcggtt ttggtttagt
     4321 atatctagcg agttccttgg cgacatttaa aatttacaaa taaagtttct ctattcaatc
     4381 gggacagtgg aaattgacta ttttatttat attaatgaac ttatttttaa tttggcttaa
     4441 gttactaagg ggtactaata gtttgagcgc agtgcatgtc atggggacat gtgcaattgt
     4501 gtgtaagcgg gaagtgatcg cggccttccg aatttggcca tgccaaataa tcccagctcg
     4561 aaaggagggg acccggcggt cagggccatg gacattgaac ttgaaaaaaa aaaacacaaa
     4621 aatatataac acaaaacgga aaatgctgtg taccgcttat gttagagaag ttgagcaacg
     4681 ggtttttcgt tttgcagtca cgatggattt ccaaattagt gtaggagggg ggaggggagg
     4741 gagggagata atgtccaggc tgccataagt ggggaataag gaaaataaaa catgaaacac
     4801 gggtcgggca atgtcatgcg gtattcggct ttgctttccg cccaagttga agtgatcctg
     4861 tgtgtaaata atgtcgaatg ttgccggtcg gttgcataag cgttagtcaa ttatgccaaa
     4921 gagatctgat ttgtggaagc tttttttgac cacttagcgc gctccgcttg atgttgtttt
     4981 gttttgtgct ggggcagaaa acttgtttca attattggga aaagtgcgta taaatcattg
     5041 ccgcaagctc tgaaaagcga aaaagaaaaa cagtaaccaa acagacaaac gcagcatccc
     5101 cccacacaat taagcaaaaa cttgaaaaca agtcaattcg aaaaaaatta taggttcaac
     5161 ggctgcagcg atcgcatcat tagttgcgtt tttagtaaat acaccatttc attacacaac
     5221 acacacaatt aattaataaa actgtactgg ttatttcaag tgtgtctttt aataagcctg
     5281 ccgatcgcaa taaattcgag cagcattgcc ggtaattttg tgcaacatat ttttcgattg
     5341 ccacaccgtg tttgtttatt tttctgtggg tgcaatgatt tagaatgcgg gcaagggatc
     5401 aagttgaacc acttctaaga aaaaatagca ttgcataaat gatatagagt ccaaaaacta
     5461 cacaaattca atagcagtaa tggttacatt agctttgaaa ttgtttttag acatccgaag
     5521 aaataagatt aaatttaaac ggcattcttt aatttgtatt ttaatatttt gagaggtttt
     5581 ccttatttaa agtgtagatt attgaggatt aatgcaaacc actttatctg cggaggtcgt
     5641 aaaacgtatt tttacccatt tgcatgttta ttatgcgtgt ggctggttgt attactttac
     5701 ttaagttttg caattttttc tttagcaagc aggtgcattt gggccaagag atatatgcga
     5761 tcgctttcgg ttcgaatttt taacatttac ttgcggcgat ggtcattaga gcattaccca
     5821 cttagggcac ccccaacatc cagttgattt tcagggacca caatatttta aataacagct
     5881 agtggaatta cctaaaagcg ctttcgtccc ttttgaaatt ttatgtaaca ctcaattata
     5941 tttatgtata tgtatgctca aaatcacctg ccaataacta gcggaaacca aatatttgac
     6001 cctcagtgaa ttgtgaatca tcggtgacgc ccaatcgaaa tccaatccta agcaattgaa
     6061 acgagcacga gttccaattt aatagtatac aaggaaacac ctgctttaaa tactctacat
     6121 agtacacgtt ataataacga tttatttgat atttctggat ttttgtctgc atgtatttca
     6181 tataatattg atttgatttt tttaatgaat tgaactaaaa aatcatatta gaacattttt
     6241 tgcagtcgcc gataaagatg aacactgttc tcagaacaca actgtcatgt attaagcttt
     6301 cagattttca gaaatttgga gagcaatgca ttctatgcac gagcctcctg gccttacaat
     6361 ttacttgttt gaaattagat cgtcaaataa agtccctaaa attaaataaa tagtagtcac
     6421 aactttaaaa taggtcttaa tcttttaggg taccgaaagg tatttcggca caaatcagcg
     6481 cagttttaaa tgtcgatgaa ggccaaaaat cataccaaac ccagcgaaag gtgatgtctg
     6541 actcattaaa ttgggggatt cgagtgtatt tattaaacat gcgtgaaaat caatcatgga
     6601 agacaaaacg caaagttggc cgatctatgg gaacagcata agccacctga ttacccgaac
     6661 actgaaccac ccgaatcact aaaaccaccg aagttggcgc gcgccttcgt tttcattttc
     6721 attggcctgt cttcgtcttc ggagaaaaaa accttcatat aaaacgcggc cgacatatta
     6781 tggccaccag tcgttaccgc gccacggtcc acagaagagg attaaaaaaa tatcacacag
     6841 ccgaaggcta gagaagaacc ccctatagct gaacatatat aaacaaatat attttttttt
     6901 attgccaaca cactttggct taagtgttaa gagtgattgt cagcttagag ctaagtgcaa
     6961 tgttccagga caaagggtgg atccttgtga ccctgatcac cttggtgacg ccgtcttggg
     7021 ctgcttacaa acttcaggag cgatatagtt ggagccagct ggactttgct ttcccgaata
     7081 cccgactaaa ggaccaagct ctggctagtg gagattatat tccgcaaaat gctctacctg
     7141 ttggagtcga acactttggc aatcggttat tcgtcactgt tccccgctgg cgtgatggga
     7201 ttccggccac tctgacctat ataaacatgg accgcagttt gacgggttca ccggagctaa
     7261 ttccgtatcc agattggcgc tcaaatacag ctggagattg cgccaacagt attaccactg
     7321 cctaccgcat taaagtggat gagtgtggtc ggctgtgggt tttggacact ggaaccgtgg
     7381 gcatcggcaa taccaccact aatccgtgcc cctatgcggt aaatgtcttt gacttgacca
     7441 cggatacgcg aattcggaga tacgagctac ctggcgtgga cacaaatcca aatactttca
     7501 tagctaacat tgccgtggat ataggcaaaa attgcgatga tgcatatgcc tattttgccg
     7561 atgaattggg atacggcttg attgcttact cctgggaact gaacaagtcc tggagattct
     7621 cggcacattc gtattttttc cccgatccat tgaggggcga tttcaatgtc gctggtatta
     7681 acttccaatg gggcgaggag ggtatatttg gtatgtccct ttcgcccatt cgatcggatg
     7741 gttatcgtac cctgtacttt agtccgttag caagtcatcg acaatttgcc gtatccacga
     7801 ggattttgag ggatgaaacc aggacggaag atagctatca tgactttgtt gccttagatg
     7861 aacggggtcc aaactcccat accacttcac gtgtgatgag cgatgatgga attgagctgt
     7921 tcaatttaat agatcaaaat gcagtgggtt gctggcactc atcaatgccg tactcaccgc
     7981 aatttcatgg cattgtggat cgcgatgacg ttggcttagt ttttccggcc gatgtgaaaa
     8041 ttgatgagaa caaaaacgtt tgggttctat ccgataggat gcccgttttc ttgctgtctg
     8101 acttggatta ttcagatact aatttccgaa tttacacggc tcccttggcc actttaattg
     8161 agaatactgt gtgtgatttg aggaataacg cctatgggcc gccaaatacc gtttcaatac
     8221 caaaacaagc cgttttgcca atgggtccac cgttatatac gaaacaatat cgtcctgtct
     8281 tgccacagaa acctcagacc agctgggctt cctcgccgcc tcctccaagt cgcacttatt
     8341 tgcccgccaa ttcaggcaat gtagtctcca gtattagtgt ctctacaaat tctgtgggtc
     8401 ctgcaggagt ggaggtgcca aaggcctata ttttcaacca gcacaacggc ataaattacg
     8461 agacaagtgg tccccatcta tttcccaccc atcaacccgc ccaaccgggt ggccaggatg
     8521 gtgggttaaa aacttatgtg aatgcccgcc aatctgggtg gtggcatcat cagcatcaag
     8581 gttaacataa tcctacacac ggtacttggg tatattctca cacactcgat tgatgtaaag
     8641 aatatttaaa gacaacaaca tagggcaaca gcggttaaaa aaaccacatg acgtatgagc
     8701 aagtggcaaa tcaatacttt atctagttat gttaagcaaa aaataacaat aaatcaactt
     8761 ttttttgaag gttaagagtt tacgcaattt tcttgagcgg aaaaagcgga aaaaatgtaa
     8821 gtatgcataa attctaaata tatcaacaac tgtacatttt ctggagtact actaccaggc
     8881 aagaaagtag gttgataaag ctatgcacaa gatcttgttt gggtgcaggg aaagttcaac
     8941 ttaatcgctc aatttgagat cgcctggtcg cttgagattc gactgtaatt gaaatttttg
     9001 cttttgatcg gagccagact tcagacgggg caaacaaaaa gactttgttg gtggtagggt
     9061 aggatccgtt gacctgcagg tcgactctag aggatcc
//
