LOCUS       sequence-338219-       11509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for CLTX T-CAR expression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, 
            Torregrosa-Allen S, Elzey BD, Won YY, Deng Q, Lian XL, Wang X, 
            Eniola-Adefeso O, Bao X
  TITLE     CAR-neutrophil mediated delivery of tumor-microenvironment 
            responsive nanodrugs for glioblastoma chemo-immunotherapy.
  JOURNAL   Nat Commun. 2023 Apr 20;14(1):2266. doi: 10.1038/s41467-023-37872-4.
  PUBMED    37080958
REFERENCE   2  (bases 1 to 11509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-20"; volume: "14(1):2266. doi"; pages: " 
            10.1038/s41467-023-37872-4"
COMMENT     Sequence Label: AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR
FEATURES             Location/Qualifiers
     source          1..11509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..840
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
     CDS             896..949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             959..1558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(959..978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1455..1475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1590..1607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1596..1820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1826..2205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2207..2484
                     /label=chicken beta-actin promoter
     intron          2485..3501
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3425..3447
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3509..3528
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    4870..5458
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4923..4943)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5341..5352)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5490..5966
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5689..5708)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6076..6095)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6141..6196
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6195..6214)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6557..6573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6581..6597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6605..6611,6612..6629,6630..6635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6650..6671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6745..7581
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7615..7634)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7616..7634)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7641..7658)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7641..7657)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7650..7672)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7795..8097
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7976..7995
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8500..8519)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9114..9133
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9494..10285,10286..10354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10117..10136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10478..11066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10967..10986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11220..11237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11354..11375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11390..11395,11396..11413,11414..11420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11428..11444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11452..11468
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11489..11507
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct
       61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
      121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
      181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
      241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
      301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
      361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
      421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta
      481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc
      541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc
      601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc
      661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca
      721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc
      781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
      901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat
      961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     1081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     1561 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1621 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1681 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1741 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1801 ggatgcggtg ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca
     1861 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1921 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1981 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2041 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2101 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2161 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     2221 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     2281 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccgggg
     2341 gggggggggg gggggggggg gsggggsggg gsgrggcgga gaggtgcggc ggcagccaat
     2401 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     2461 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     2521 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     2581 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     2641 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     2701 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     2761 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     2821 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     2881 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     2941 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     3001 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     3061 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     3121 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     3181 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     3241 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     3301 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     3361 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     3421 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     3481 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     3541 tttggcaaag aattcgtcga cgccaccatg cttctcctgg tgacaagcct tctgctctgt
     3601 gagttaccac acccagcatt cctcctgatc ccaatgtgca tgccttgctt caccaccgac
     3661 caccagatgg ccagaaagtg cgatgactgc tgcggcggca agggcagagg caagtgctac
     3721 ggcccccagt gtctgtgcag agagtccaaa tatggtcccc catgcccacc atgcccagca
     3781 cctgagttcc tggggggacc atcagtcttc ctgttccccc caaaacccaa ggacactctc
     3841 atgatctccc ggacccctga ggtcacgtgc gtggtggtgg acgtgagcca ggaagacccc
     3901 gaggtccagt tcaactggta cgtggatggc gtggaggtgc ataatgccaa gacaaagccg
     3961 cgggaggagc agttcaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag
     4021 gactggctga acggcaagga gtacaagtgc aaggtctcca acaaaggcct cccgtcctcc
     4081 atcgagaaaa ccatctccaa agccaaaggg cagccccgag agccacaggt gtacaccctg
     4141 cccccatccc aggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
     4201 ttctacccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac
     4261 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caggctaacc
     4321 gtggacaaga gcaggtggca ggaggggaat gtcttctcat gctccgtgat gcatgaggct
     4381 ctgcacaacc actacacaca gaagagcctc tccctgtccc taggtaaaat cattgtggct
     4441 gtggtcattg cgactgctgt agcagccatt gttgctgctg tagtggcctt gatctacaga
     4501 gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagcagctc
     4561 tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc
     4621 cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat
     4681 gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc
     4741 cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc
     4801 tacgacgccc ttcacatgca ggccctgccc cctcgctgaa cgcgtgaatt cgatatcaag
     4861 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4921 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4981 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5041 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5101 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5161 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5221 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5281 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5341 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5401 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     5461 cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
     5521 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     5581 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     5641 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     5701 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     5761 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     5821 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     5881 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     5941 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccaggcgc gtgaattcac
     6001 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     6061 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     6121 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     6181 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     6241 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     6301 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     6361 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     6421 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     6481 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     6541 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6601 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6661 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6721 agcggatcga cagtactaag ctttactagg gacaggattg gtgacagaaa agccccatcc
     6781 ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc ctgttaggca
     6841 gattccttat ctggtgacac acccccattt cctggagcca tctctctcct tgccagaacc
     6901 tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct tggcaggggg
     6961 tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc tgcgctaccc
     7021 tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg atcctggtgc
     7081 tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa tcagaataag
     7141 ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta tattgttcct
     7201 ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct ggcagggctg
     7261 tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg cgtcctaggt
     7321 gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc tttccttaaa
     7381 gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc gcttccctaa
     7441 ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg cgctcaggct
     7501 tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc ccttccctac
     7561 aggggttcct ggctctgctc taagggcgaa ttcgcggccg ctaaattcaa ttcgccctat
     7621 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7681 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7741 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctatacgt acggcagttt
     7801 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     7861 attattgaca cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     7921 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     7981 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     8041 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     8101 tcaggcatga gattatcaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt
     8161 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     8221 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
     8281 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     8341 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     8401 aggggatcaa gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     8461 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     8521 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     8581 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     8641 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     8701 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     8761 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     8821 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     8881 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     8941 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     9001 gtgacccatg catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     9061 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     9121 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     9181 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     9241 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     9301 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9361 tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca
     9421 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9481 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9541 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9601 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9661 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9721 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9781 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9841 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9901 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9961 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10021 taagatgctt ttctgtgact ggtgaatact caaccaagtc attctgagaa tagtgtatgc
    10081 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10141 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10201 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10261 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10321 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10381 gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact
    10441 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10501 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10561 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10621 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10681 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10741 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10801 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10861 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10921 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10981 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11041 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11101 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11161 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11221 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11281 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    11341 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11401 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11461 gctatgacca tgattacgcc aagctcagaa ttaaccctca ctaaaggga
//
