LOCUS       sequence-338222-       11401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, 
            Torregrosa-Allen S, Elzey BD, Won YY, Deng Q, Lian XL, Wang X, 
            Eniola-Adefeso O, Bao X
  TITLE     CAR-neutrophil mediated delivery of tumor-microenvironment 
            responsive nanodrugs for glioblastoma chemo-immunotherapy.
  JOURNAL   Nat Commun. 2023 Apr 20;14(1):2266. doi: 10.1038/s41467-023-37872-4.
  PUBMED    37080958
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-20"; volume: "14(1):2266. doi"; pages: " 
            10.1038/s41467-023-37872-4"
COMMENT     Sequence Label: AAVS1-Puro CAG-CLTX CD32a ITAM CAR
FEATURES             Location/Qualifiers
     source          1..11401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    37..840
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
     CDS             896..949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             959..1558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(959..978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1455..1475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1590..1607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1596..1820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1826..2205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2207..2484
                     /label=chicken beta-actin promoter
     intron          2485..3501
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3425..3447
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3509..3528
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    4762..5350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4815..4835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5233..5244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5382..5858
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5581..5600)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5968..5987)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6033..6088
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6087..6106)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6449..6465)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6473..6489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6497..6503,6504..6521,6522..6527))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6542..6563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6637..7473
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7507..7526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7508..7526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7533..7550)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7533..7549)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7542..7564)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7687..7989
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7868..7887
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8392..8411)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9006..9025
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9386..10177,10178..10246))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10009..10028
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10370..10958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10859..10878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11112..11129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11246..11267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11282..11287,11288..11305,11306..11312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11320..11336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11344..11360
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11381..11399
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagctagtc ctgcaggttt aaacgaattc gccctttgct ttctctgacc agcattctct
       61 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
      121 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
      181 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
      241 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
      301 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
      361 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
      421 tcccgcctcc ccttcttgta ggcctgcatc atcaccgttt ttctggacaa ccccaaagta
      481 ccccgtctcc ctggctttag ccacctctcc atcctcttgc tttctttgcc tggacacccc
      541 gttctcctgt ggattcgggt cacctctcac tcctttcatt tgggcagctc ccctaccccc
      601 cttacctctc tagtctgtgc tagctcttcc agccccctgt catggcatct tccaggggtc
      661 cgagagctca gctagtcttc ttcctccaac ccgggcccct atgtccactt caggacagca
      721 tgtttgctgc ctccagggat cctgtgtccc cgagctggga ccaccttata ttcccagggc
      781 cggttaatgt ggctctggtt ctgggtactt ttatctgtcc cctccacccc acagtggggc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag agatctggca gcggagaggg
      901 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggctcgagat
      961 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1021 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     1081 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1141 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1201 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1261 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1321 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1381 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1441 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1501 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc
     1561 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1621 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1681 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1741 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1801 ggatgcggtg ggctctatgg gtctcgacat tgattattga ctagttatta atagtaatca
     1861 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1921 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1981 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2041 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2101 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2161 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     2221 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     2281 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccgggg
     2341 gggggggggg ggggsggggg gsggggsggg gsgrggcgga gaggtgcggc ggcagccaat
     2401 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     2461 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     2521 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     2581 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     2641 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     2701 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     2761 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     2821 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     2881 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     2941 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     3001 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     3061 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     3121 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     3181 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     3241 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     3301 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     3361 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     3421 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     3481 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     3541 tttggcaaag aattcgtcga cgccaccatg cttctcctgg tgacaagcct tctgctctgt
     3601 gagttaccac acccagcatt cctcctgatc ccaatgtgca tgccttgctt caccaccgac
     3661 caccagatgg ccagaaagtg cgatgactgc tgcggcggca agggcagagg caagtgctac
     3721 ggcccccagt gtctgtgcag agagtccaaa tatggtcccc catgcccacc atgcccagca
     3781 cctgagttcc tggggggacc atcagtcttc ctgttccccc caaaacccaa ggacactctc
     3841 atgatctccc ggacccctga ggtcacgtgc gtggtggtgg acgtgagcca ggaagacccc
     3901 gaggtccagt tcaactggta cgtggatggc gtggaggtgc ataatgccaa gacaaagccg
     3961 cgggaggagc agttcaacag cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag
     4021 gactggctga acggcaagga gtacaagtgc aaggtctcca acaaaggcct cccgtcctcc
     4081 atcgagaaaa ccatctccaa agccaaaggg cagccccgag agccacaggt gtacaccctg
     4141 cccccatccc aggaggagat gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc
     4201 ttctacccca gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac
     4261 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag caggctaacc
     4321 gtggacaaga gcaggtggca ggaggggaat gtcttctcat gctccgtgat gcatgaggct
     4381 ctgcacaacc actacacaca gaagagcctc tccctgtccc taggtaaaat cattgtggct
     4441 gtggtcattg cgactgctgt agcagccatt gttgctgctg tagtggcctt gatctactgc
     4501 aggaaaaagc ggatttcagc caattccact gatcctgtga aggctgccca atttgagcca
     4561 cctggacgtc aaatgattgc catcagaaag agacaacttg aagaaaccaa caatgactat
     4621 gaaacagctg acggcggcta catgactctg aaccccaggg cacctactga cgatgataaa
     4681 aacatctacc tgactcttcc tcccaacgac catgtcaaca gtaataacta aacgcgtgaa
     4741 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     4801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5161 tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc
     5221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5341 gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc
     5401 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
     5461 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
     5521 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
     5581 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
     5641 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
     5701 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
     5761 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
     5821 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccaggc
     5881 gcgtgaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5941 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     6001 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     6061 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     6121 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     6181 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     6241 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     6301 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     6361 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     6421 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6481 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6541 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6601 acctgtcgtg ccagcggatc gacagtacta agctttacta gggacaggat tggtgacaga
     6661 aaagccccat ccttaggcct cctccttcct agtctcctga tattgggtct aacccccacc
     6721 tcctgttagg cagattcctt atctggtgac acacccccat ttcctggagc catctctctc
     6781 cttgccagaa cctctaaggt ttgcttacga tggagccaga gaggatcctg ggagggagag
     6841 cttggcaggg ggtgggaggg aaggggggga tgcgtgacct gcccggttct cagtggccac
     6901 cctgcgctac cctctcccag aacctgagct gctctgacgc ggctgtctgg tgcgtttcac
     6961 tgatcctggt gctgcagctt ccttacactt cccaagagga gaagcagttt ggaaaaacaa
     7021 aatcagaata agttggtcct gagttctaac tttggctctt cacctttcta gtccccaatt
     7081 tatattgttc ctccgtgcgt cagttttacc tgtgagataa ggccagtagc cagccccgtc
     7141 ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt gtgagaatgg
     7201 tgcgtcctag gtgttcacca ggtcgtggcc gcctctactc cctttctctt tctccatcct
     7261 tctttcctta aagagtcccc agtgctatct gggacatatt cctccgccca gagcagggtc
     7321 ccgcttccct aaggccctgc tctgggcttc tgggtttgag tccttggcaa gcccaggaga
     7381 ggcgctcagg cttccctgtc ccccttcctc gtccaccatc tcatgcccct ggctctcctg
     7441 ccccttccct acaggggttc ctggctctgc tctaagggcg aattcgcggc cgctaaattc
     7501 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     7561 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7621 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac
     7681 gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     7741 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
     7801 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     7861 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     7921 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     7981 ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg
     8041 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     8101 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     8161 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     8221 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     8281 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     8341 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     8401 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     8461 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     8521 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     8581 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     8641 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8701 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8761 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8821 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     8881 gaggatctcg tcgtgaccca tgcatggcga tgcctgcttg ccgaatatca tggtggaaaa
     8941 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     9001 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     9061 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     9121 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac
     9181 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
     9241 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa
     9301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9901 tcatgccatc cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag
     9961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10261 aatattattg aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt
    10321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10501 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10981 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11041 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11101 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    11161 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    11221 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11281 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11341 acacaggaaa cagctatgac catgattacg ccaagctcag aattaaccct cactaaaggg
    11401 a
//
