LOCUS       sequence-338258-       12312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of murine Arid3a cDNA with dTomato 
            as reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 12312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A....
FEATURES             Location/Qualifiers
     source          1..12312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5190..5764,5765..5767,5768..5776)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5357..5374)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5584..5603
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5783..6484
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5916..5934)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6184..6203
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6378..6401
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6509..7008
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6544..6567)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6941..6960
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7015..7024
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7021..7023,7024..7026,7027..7737)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7750..7806
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7807..8553
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8580..9168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8633..8653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9051..9062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9240..9473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9551..9672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9588..9607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9642..9661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9712..9847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9867..9886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9868..9886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9896..9913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9896..9912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9905..9927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10054..10509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10141..10160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10351..10372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10535..10639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10640..10708,10709..11500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10858..10877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11671..12259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12160..12179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
     3061 cagtttactc cctatcagtg atagagaacg tatmwrsagt ttactcccta tcagtgatag
     3121 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagac cggtatgaag ttgcaagccg tcatggaaac tctcattcag cggcaacaga
     3421 gagcacgaca agaactggaa gctcgccaag cccctcctcc tccccctcct gaacctacag
     3481 gagttcgcgc tcgaacaacc atgactgacg aggacagaga gccagaaaat gcccggatgc
     3541 atcggactca gatggcagcc ttggccgcca tgcgcgctgc tgctgctgga ttgggtcacc
     3601 cttctagtcc cggaggctct gaagacgggc ccccaatctc cggtgatgaa gacaccgcca
     3661 gggagggtac tctttctagt ccagcactcc atggcagcgt gttggagggt gctggtcatg
     3721 ccgagggaga tcggcatttg atggatgttg ggtcagacga tgatgataca aagtctaaat
     3781 gggaagagca ggagttggaa gaattgggag aggaggaaga ggaagaagag gaggaggacg
     3841 attttgagga ggaagaagaa gaggaggagg gtttgggtcc tcccgaatcc gcctccctgg
     3901 ggacagctgg actcttcacc cgaaaggccc ctccagcaca ggcatttcgg ggtgacggag
     3961 ggccccgcat gctctctgga cccgaaagat tgggtcctgg ccccgcccac ccaagtcaca
     4021 tggcctcaca gatgcctcca cccgaccatg gcgactggac tttcgaggaa caatttaaac
     4081 agctttatga gctcgacgca gatccaaaac ggaaagagtt tctggatgat ctcttctcct
     4141 ttatgcagaa gcgaggcacc cctgtaaaca ggattccaat aatggctaag caagtactgg
     4201 acctgtttat gctgtatgtc cttgtaactg agaagggagg gctcgtcgaa gtaatcaaca
     4261 agaaactttg gagagaaatt acaaagggat tgaatttgcc taccagtata acctcagctg
     4321 cattcacact gagaactcag tacatgaaat atctctatcc ctatgaatgc gaacgcaggg
     4381 gtctttcttc tcctaatgaa ctccaagctg ctattgatag taataggaga gaaggcaggc
     4441 gccagagctt cgggggaagc ctcttcgctt atagccccag tggagcccat tcaatgctcc
     4501 ctagtccaaa actccctgtc actccactcg gactggctgc ttctactaat ggctcatcaa
     4561 ttaccccagc acctaaaatc aagaaggaag aagactcagc aatcccaatt accgtgcccg
     4621 gtaggttgcc tgtgtccttg gccggccatc ctgttgtggc cgcacaagca gcagcagtac
     4681 aggccgctgc cgcacaggcc gctgtcgctg cccaagcagc tgcactcgaa caacttcgag
     4741 aaaaactgga atccaccgag ccacctgaga agaaaatggc tctggttgca gatgagcaac
     4801 agagacttat gcagcgcgct gttcagcagt cctttctggc aatgaccgcc caacttccca
     4861 tgaacattag aattaatagc caagcttccg aaagccgcca agatagtgcc gtttccttga
     4921 catctgcaaa tggttccaat agtatttcaa tgagtgttga aatgaatggg atagtatata
     4981 ctggcgtcct ttttgcccag cctcctcctc ctacagcacc ttcagcacca ggtaagggcg
     5041 gagtcagctc tattggtaca aataccacta ccggaagtcg cactggggcc agtgggtcaa
     5101 ccgtctctgg cgggcaggtg ggacttcctg gcgtttctac tcccaccatg agttccacca
     5161 gtaacaattc cctgccataa acgcgttcgc ccctctccct cccccccccc taacgttact
     5221 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5281 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5341 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5401 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5461 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5521 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     5581 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     5641 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     5701 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5761 taatatggcc acaaccggat ccatggtgag caagggcgag gaggtcatca aagagttcat
     5821 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     5881 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     5941 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     6001 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     6061 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     6121 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     6181 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     6241 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     6301 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     6361 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6421 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     6481 caagtgagcg atatcgcgta attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6541 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6601 cgcacacatt ccacatccac cggaaggcgc caaccggctc cgttctttgg tggccccttc
     6661 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6721 tgcaggacgt gacaaatgga agtagcacgt gtcactagtc tcgtgcagat ggacagcacc
     6781 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6841 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6901 aggggcgggg cgggcgcccg aaggtccacc ggaggcccgg cattctgcac gcttcaaaag
     6961 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcggc tagcgccacc
     7021 atgggatcta agggcgagga actgttcacc ggcgtggtgc ccatcctggt ggaactggat
     7081 ggcgacgtga acggccacaa gttctctgtg cggggagagg gcgaaggcga cgccacaaat
     7141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ttggcctacc
     7201 ctcgtgacca cactgaccta cggcgtgcag tgcttcagca gataccccga ccatatgaag
     7261 cggcacgact tcttcaagag cgccatgccc gagggctacg tgcaggaacg gaccatcagc
     7321 ttcaaggacg acggcaccta caagaccaga gccgaagtga agttcgaggg cgacaccctc
     7381 gtgaaccgga tcgagctgaa gggcatcgat ttcaaagagg acggcaacat cctgggccac
     7441 aagctggagt acaacttcaa cagccacaat gtgtatatca ccgccgacaa gcagaagaac
     7501 ggcatcaagg ccaacttcaa gatccggcac aacgtggaag atggcagcgt gcagctggcc
     7561 gaccactacc agcagaacac ccccatcgga gatggccccg tgctgctgcc cgacaaccac
     7621 tacctgagca cccagagcgt gctgagcaag gaccccaacg agaagcggga ccacatggtg
     7681 ctgctggaat tcgtgaccgc cgctggcatc acccacggca tggacgagct gtataaggga
     7741 ggaagcggcg ccaccaattt cagcctgctg aaacaggccg gcgacgtgga agagaaccct
     7801 ggccctatga gccggctgga caagagcaaa gtgatcaaca gcgccctgga actgctgaac
     7861 ggcgtgggca tcgagggcct gaccacccgg aagctggccc agaaactggg cgtggaacag
     7921 cccaccctgt actggcacgt gaagaacaag cgggccctgc tggacgccct gcccatcgag
     7981 atgctggacc ggcaccacac ccacagctgc cctctggaag gcgagagctg gcaggacttc
     8041 ctgcggaaca acgccaagag ctacagatgc gccctgctga gccaccggga cggcgccaaa
     8101 gtgcacctgg gcaccagacc caccgagaag cagtacgaga cactggaaaa ccagctggcc
     8161 ttcctgtgcc agcagggctt cagcctggaa aacgccctgt acgccctgag cgccgtgggc
     8221 cacttcaccc tgggctgcgt gctggaagaa caggaacacc aggtcgccaa agaggaaaga
     8281 gagacaccca ccaccgacag catgcccccc ctgctgaagc aggccatcga gctgttcgac
     8341 agacagggcg ccgagcccgc cttcctgttc ggcctggaac tgatcatctg cggcctcgag
     8401 aagcagctga agtgcgagag cggcggaccc accgacgccc tggacgactt cgacctggac
     8461 atgctgcccg ccgatgccct ggatgatttt gatctggata tgctccctgc tgacgctctc
     8521 gatgacttcg atctcgatat gctgccaggc taatgtacaa gtgatcgcgt taagtcgaca
     8581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     9001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     9181 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     9241 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     9301 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9361 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9421 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     9481 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9541 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9601 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9661 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     9721 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9781 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9841 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     9901 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     9961 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    10021 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
    10081 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    10141 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    10201 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    10261 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    10321 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    10381 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    10441 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10501 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10561 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10621 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10681 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10741 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10801 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10861 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    10921 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10981 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    11041 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    11101 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    11161 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11221 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11281 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11341 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11401 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11461 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11521 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11581 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    12121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    12181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    12241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    12301 ctggcctttt gc
//
