LOCUS       sequence-338263-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of miR-125b-2 with dTomato as 
            reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2106..2485
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        2542..2733
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2565..2585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2752..2978
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2979..3159
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3206..3331
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3824..4057
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4242..4286
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4499..4616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4662..5040
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    4670..4688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4706..4724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4742..4760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4778..4796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4814..4832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4850..4868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4886..4904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4949..4973
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5047..5056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5053..5754
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5186..5204)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5454..5473
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5648..5671
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6173..6672
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6208..6231)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6605..6624
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6679..6688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6685..6687,6688..6690,6691..7401)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7414..7470
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7471..8217
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8244..8832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8297..8317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8715..8726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8904..9137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9215..9336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9252..9271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9306..9325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9376..9511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9531..9550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9532..9550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9560..9577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9560..9576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9569..9591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9718..10173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9805..9824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10015..10036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10199..10303
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcggtacc gtcgacctgc
     2101 aggttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2161 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2221 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2281 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2341 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2401 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2461 cgtattagtc atcgctatta ccatggcgcg ccatgcaggg cctgaaataa cctctgaaag
     2521 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     2581 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     2641 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2701 catgcatctc aattagtcag caaccatagt cccttaagag gcgcgcctta atgtagtctt
     2761 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     2821 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     2881 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     2941 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     3001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3181 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3241 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3301 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3361 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3421 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3481 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3541 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3601 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3661 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     3721 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     3781 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     3841 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     3901 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     3961 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     4021 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     4081 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     4141 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     4201 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     4261 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     4321 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     4381 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     4441 tcccaacccc gaggggacaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     4501 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4561 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     4621 tttattacag ggacagcaga gatccacttt ggcgccggct cgagtttact ccctatcagt
     4681 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     4741 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     4801 atrwccagtt tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt
     4861 gatagagaac gtatatccag tttactccct atcagtgata gagaacgtat aagctttagg
     4921 cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4981 gcaattccac aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa
     5041 ggatccgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     5101 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     5161 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     5221 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     5281 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     5341 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     5401 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     5461 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     5521 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     5581 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     5641 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     5701 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtgagct
     5761 agccgtctca gatccaatta gtctataaat ggtcgtcgtg attactcagc tcatcctaac
     5821 ttttatatat catatatatt tctactgaag tattttaaat agtatttaga ggtaaaagtc
     5881 taagtgaacc caactgtaat ttctaagcta tccttatttc tggaagaaga attctaccgc
     5941 atcaaaccag acttttccta gtccctgaga ccctaacttg tgaggtattt tagtaacatc
     6001 acaagtcagg ctcttgggac ctaggcggag gggaaccagc agctttggac cttattgatt
     6061 gtctgcagtt accaccagaa caaaagaaca tacatagatt ctgcctagga gaaaagaaca
     6121 atgcttttct ttatcagcaa cgttttcacc accggtaacg cgtaattcta ccgggtaggg
     6181 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     6241 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggaag gcgccaaccg
     6301 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     6361 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtgtcact
     6421 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     6481 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     6541 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc caccggaggc
     6601 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     6661 ccgggccttt cggctagcgc caccatggga tctaagggcg aggaactgtt caccggcgtg
     6721 gtgcccatcc tggtggaact ggatggcgac gtgaacggcc acaagttctc tgtgcgggga
     6781 gagggcgaag gcgacgccac aaatggcaag ctgaccctga agttcatctg caccaccggc
     6841 aagctgcccg tgccttggcc taccctcgtg accacactga cctacggcgt gcagtgcttc
     6901 agcagatacc ccgaccatat gaagcggcac gacttcttca agagcgccat gcccgagggc
     6961 tacgtgcagg aacggaccat cagcttcaag gacgacggca cctacaagac cagagccgaa
     7021 gtgaagttcg agggcgacac cctcgtgaac cggatcgagc tgaagggcat cgatttcaaa
     7081 gaggacggca acatcctggg ccacaagctg gagtacaact tcaacagcca caatgtgtat
     7141 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg gcacaacgtg
     7201 gaagatggca gcgtgcagct ggccgaccac taccagcaga acacccccat cggagatggc
     7261 cccgtgctgc tgcccgacaa ccactacctg agcacccaga gcgtgctgag caaggacccc
     7321 aacgagaagc gggaccacat ggtgctgctg gaattcgtga ccgccgctgg catcacccac
     7381 ggcatggacg agctgtataa gggaggaagc ggcgccacca atttcagcct gctgaaacag
     7441 gccggcgacg tggaagagaa ccctggccct atgagccggc tggacaagag caaagtgatc
     7501 aacagcgccc tggaactgct gaacggcgtg ggcatcgagg gcctgaccac ccggaagctg
     7561 gcccagaaac tgggcgtgga acagcccacc ctgtactggc acgtgaagaa caagcgggcc
     7621 ctgctggacg ccctgcccat cgagatgctg gaccggcacc acacccacag ctgccctctg
     7681 gaaggcgaga gctggcagga cttcctgcgg aacaacgcca agagctacag atgcgccctg
     7741 ctgagccacc gggacggcgc caaagtgcac ctgggcacca gacccaccga gaagcagtac
     7801 gagacactgg aaaaccagct ggccttcctg tgccagcagg gcttcagcct ggaaaacgcc
     7861 ctgtacgccc tgagcgccgt gggccacttc accctgggct gcgtgctgga agaacaggaa
     7921 caccaggtcg ccaaagagga aagagagaca cccaccaccg acagcatgcc ccccctgctg
     7981 aagcaggcca tcgagctgtt cgacagacag ggcgccgagc ccgccttcct gttcggcctg
     8041 gaactgatca tctgcggcct cgagaagcag ctgaagtgcg agagcggcgg acccaccgac
     8101 gccctggacg acttcgacct ggacatgctg cccgccgatg ccctggatga ttttgatctg
     8161 gatatgctcc ctgctgacgc tctcgatgac ttcgatctcg atatgctgcc aggctaatgt
     8221 acaagtgatc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     8281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     8461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     8641 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8821 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     8881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8941 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9001 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9061 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9121 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     9181 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     9241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     9361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9481 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     9541 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     9601 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     9661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     9721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9901 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
    10021 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
    10081 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    10141 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
    10201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10261 ataaccctga taaatgcttc aataatattg aaaaaggaag agt
//
