LOCUS       sequence-338264-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for expression of kinase inactive PAK2 with a CFP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Whalley HJ, Porter AP, Diamantopoulou Z, White GR, Castaneda-Saucedo
            E, Malliri A
  TITLE     Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal 
            Pak and promote mitotic spindle formation.
  JOURNAL   Nat Commun. 2015 Jun 16;6:7437. doi: 10.1038/ncomms8437.
  PUBMED    26078008
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-16"; volume: "6:7437. doi"; pages: " 
            10.1038/ncomms8437"
COMMENT     Sequence Label: pRetro-XT-PAK2-CFP(puro)-T402A
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1676..1990
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1684..1702
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1720..1738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1755..1773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1791..1809
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1827..1845
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1862..1880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1898..1916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1963..1987
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2014..2023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2020..2022,2023..2025,2026..2736)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDLNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2065..2086)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2326..2345)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2673..2694
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2800..2824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4432..4919
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4467..4490)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4852..4871
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4940..5539
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4940..4959)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5436..5456
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5720..6145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6248..6270)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6406..6426)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6411..6740
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6591..6726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6762..6781
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6862..6879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7033..7621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7113..7132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7792..8583,8584..8652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8415..8434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8653..8757)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8825..8843
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctgaggcc ctttcgtctt cactcgagtt
     1681 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     1741 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     1801 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     1861 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     1921 atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg
     1981 tcagatcgcc tggagaagga tccgcggccg ctcgccacca tggtgagcaa gggcgaggag
     2041 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2101 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2161 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     2221 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2281 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2341 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2401 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     2461 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     2521 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2581 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2641 ctgagcaaag acctcaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2701 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa
     2761 gcttcgaatt ctgcagtcga cggtaccgcg ggcccatcaa caagtttgta caaaaaagca
     2821 ggcttgatgt ctgataacgg agaactggaa gataagcctc cagcacctcc tgtgcgaatg
     2881 agcagcacca tctttagcac tggaggcaaa gaccctttgt cagccaatca cagtttgaaa
     2941 cctttgccct ctgttccaga agagaaaaag cccaggcata aaatcatctc catattctca
     3001 ggcacagaga aaggaagtaa aaagaaagaa aaggaacggc cagaaatttc tcctccatct
     3061 gattttgagc acaccatcca tgttggcttt gatgctgtta ctggagaatt cactggcatg
     3121 ccagaacagt gggctcgatt actacagacc tccaatatca ccaaactaga gcaaaagaag
     3181 aatcctcagg ctgtgctgga tgtcctaaag ttctacgact ccaacacagt gaagcagaaa
     3241 tatctgagct ttactcctcc tgagaaagat ggctttcctt ctggaacacc agcactgaat
     3301 gccaagggaa cagaagcacc cgcagtagtg acagaggagg aggatgatga tgaagagact
     3361 gctcctcccg ttattgcccc gcgaccggat catacgaaat caatttacac acggtctgta
     3421 attgaccctg ttcctgcacc agttggtgat tcacatgttg atggtgctgc caagtcttta
     3481 gacaaacaga aaaagaagac taagatgaca gatgaagaga ttatggagaa attaagaact
     3541 atcgtgagca taggtgaccc taagaaaaaa tatacaagat atgaaaaaat tggacaaggg
     3601 gcttctggta cagttttcac tgctactgac gttgcactgg gacaggaggt tgctatcaaa
     3661 caaattaatt tacagaaaca gccaaagaag gaactgatca ttaacgagat tctggtgatg
     3721 aaagaattga aaaatcccaa catcgttaac tttttggaca gttacctggt aggagatgaa
     3781 ttgtttgtgg tcatggaata ccttgctggg gggtcactca ctgatgtggt aacagaaacg
     3841 tgcatggatg aagcacagat tgctgctgta tgcagagagt gtttacaggc attggagttt
     3901 ttacatgcta atcaagtgat ccacagagac atcaaaagtg acaatgtact tttgggaatg
     3961 gaaggatctg ttaagctcac tgactttggt ttctgtgccc agatcacccc tgagcagagc
     4021 aaacgcagtg ccatggtcgg aacgccatac tggatggcac cagaggtggt tacacggaaa
     4081 gcttatggcc ctaaagtcga catatggtct ctgggtatca tggctattga gatggtagaa
     4141 ggagagcctc catacctcaa tgaaaatccc ttgagggcct tgtacctaat agcaactaat
     4201 ggaaccccag aacttcagaa tccagagaaa ctttccccaa tatttcggga tttcttaaat
     4261 cgatgtttgg aaatggatgt ggaaaaaagg ggttcagcca aagaattatt acagcatcct
     4321 ttcctgaaac tggccaaacc gttatctagc ttgacaccac tgatcatggc agctaaagaa
     4381 gcaatgaaga gtaaccgtta agacccagct cgcgaacgcg tgaattctac cgggtagggg
     4441 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     4501 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     4561 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     4621 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     4681 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     4741 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
     4801 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     4861 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     4921 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4981 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5041 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5101 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5161 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     5221 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     5281 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5341 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5401 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5461 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5521 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     5581 acgaccccat gcatcggcgt ctcgagatat cagtggtcca ggctctagtt ttgactcaac
     5641 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
     5701 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     5761 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     5821 ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt ccagggtgcc
     5881 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5941 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     6001 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     6061 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     6121 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     6181 gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg
     6241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     6361 ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat gtgtgtcagt
     6421 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6481 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6541 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6661 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6721 gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag agcagattgt
     6781 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6841 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7441 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8101 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8401 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8761 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     8821 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc cattgcatac
     8881 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
     8941 t
//
