LOCUS       sequence-338270-        7968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentiviral vector for doxycycline-inducible expression of
            FLAG-tagged, codon-optimized, full-length SRSF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Campbell AE, Dyle MC, Albanese R, Matheny T, Sudheendran K, Cortazar
            MA, Forman T, Fu R, Gillen AE, Caruthers MH, Floor SN, Calviello L, 
            Jagannathan S
  TITLE     Compromised nonsense-mediated RNA decay results in truncated 
            RNA-binding protein production upon DUX4 expression.
  JOURNAL   Cell Rep. 2023 Jun 13;42(6):112642. doi: 
            10.1016/j.celrep.2023.112642.
  PUBMED    37314931
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-13"; volume: "42(6):112642. doi"; pages: " 
            10.1016/j.celrep.2023.112642"
COMMENT     Sequence Label: pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized...
FEATURES             Location/Qualifiers
     source          1..7968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        618..1128
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1047..1065
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1157..1552
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1568..1621
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1622..2239,2240..2245,2246..2281,2282..2284,2285..2320,
                     2321..2323,2324..2359,2360..2368)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2408..2428)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2826..2837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3042..3275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3347..3468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3384..3403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3438..3457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3508..3643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3643..3714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3715..3783,3784..4575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3933..3952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4746..5334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5235..5254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5488..5505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5622..5643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5658..5663,5664..5681,5682..5688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5696..5712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5701..5723
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5720..5736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5720..5736
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5755..5775
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5757..5775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5803..6029
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6030..6210
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6257..6382
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6875..7108
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7293..7337
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7550..7667
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(7721..7968,1..67)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7729..7747
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7765..7783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7800..7818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7836..7854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7872..7890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7907..7925
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7943..7961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
       61 gatcgcctgg agaattggct agcgaattca tggactacaa agatgacgac gataaaatgc
      121 acagggactc ctgccccctt gactgcaagg tttacgtggg caaccttgga aataatggta
      181 acaagacaga actcgaacgg gccttcggct attatggacc cttgcgctct gtatgggtcg
      241 cgagaaatcc tccaggcttt gcattcgtag aatttgaaga tccacgagac gcggcggacg
      301 cagttcgaga gctggacggt cgaacattgt gcggatgtcg cgtgagggtt gaactttcta
      361 acggtgaaaa gcggagccgc aatcggggtc caccacccag ttggggcaga cgccctcgcg
      421 acgattaccg ccgcagaagt cctccaccta ggaggcgatc cccccgacga cggtcattct
      481 ctaggtctcg gagccgctcc cttagccgag accgacggag ggagagatcc ctgtcaaggg
      541 agaggaatca caaaccctca cgcagttttt cccggagcag gagccgatct cgatctaatg
      601 aacgaaagta gggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg
      661 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
      721 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
      781 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
      841 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
      901 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
      961 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     1021 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     1081 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagca attcacccgc
     1141 gcgcgggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     1201 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     1261 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     1321 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     1381 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     1441 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     1501 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcttcgaagg
     1561 aagcggagaa ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa accctggtcc
     1621 aatgtctaga ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     1681 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     1741 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     1801 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     1861 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     1921 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     1981 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     2041 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     2101 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     2161 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     2221 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     2281 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     2341 ttttgacctt gacatgctcc ccgggtaact aagtaaggat cgatccaaga tatcgtattc
     2401 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2461 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2521 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2581 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2641 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2701 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2761 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2821 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2881 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     2941 cgcctgtttc gcctcggcgt ccggactaga ggtaccttta agaccaatga cttacaaggc
     3001 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3061 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     3121 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3181 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3241 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     3301 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     3361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     3481 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3541 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3601 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctttcaaa tatgtatccg
     3661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     4921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     5761 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     5821 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     5881 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     5941 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     6001 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6061 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6121 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6181 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6241 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6301 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6361 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6421 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6481 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6541 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6601 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6661 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6721 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6781 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     6841 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     6901 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     6961 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7021 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7081 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7141 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7201 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7261 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7321 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7381 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7441 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7501 cgaggggaca attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
     7561 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     7621 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     7681 gggacagcag agatccactt tggccgcgaa tcgatatgtc gagtttactc cctatcagtg
     7741 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact
     7801 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     7861 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat
     7921 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatg
//
