LOCUS       sequence-338274-        7848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentiviral vector for doxycycline-inducible expression of
            FLAG-tagged, codon-optimized, truncated SRSF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Campbell AE, Dyle MC, Albanese R, Matheny T, Sudheendran K, Cortazar
            MA, Forman T, Fu R, Gillen AE, Caruthers MH, Floor SN, Calviello L, 
            Jagannathan S
  TITLE     Compromised nonsense-mediated RNA decay results in truncated 
            RNA-binding protein production upon DUX4 expression.
  JOURNAL   Cell Rep. 2023 Jun 13;42(6):112642. doi: 
            10.1016/j.celrep.2023.112642.
  PUBMED    37314931
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-13"; volume: "42(6):112642. doi"; pages: " 
            10.1016/j.celrep.2023.112642"
COMMENT     Sequence Label: pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-B...
FEATURES             Location/Qualifiers
     source          1..7848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        37..351
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     324..348
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             377..400
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        782..1292
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1211..1229
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1321..1716
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1732..1785
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1786..2403,2404..2409,2410..2445,2446..2448,2449..2484,
                     2485..2487,2488..2523,2524..2532)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2572..2592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2990..3001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3206..3439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3511..3632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3548..3567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3602..3621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3672..3807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3807..3878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3879..3947,3948..4739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4097..4116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4910..5498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5399..5418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5652..5669
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5786..5807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5822..5827,5828..5845,5846..5852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5860..5876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5865..5887
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5884..5900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5884..5900
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5919..5939
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5921..5939
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5967..6193
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6194..6374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6421..6546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7039..7272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7457..7501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7714..7831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta tcagtgatag
       61 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
      241 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
      301 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
      361 ggctagcgaa ttcatggact acaaagatga cgacgataaa atgcaccgag actcctgccc
      421 gctggattgc aaggtatatg tagggaatct cggcaataat ggcaacaaga cggaacttga
      481 aagagcattt ggctattatg gccctctgcg gagtgtatgg gttgccagaa acccgccagg
      541 tttcgcgttc gtcgaatttg aagacccacg agacgccgct gacgcggtta gggagctgga
      601 cgggcgcaca ctctgtggct gcagggtgag ggtggaactc agcaatggcg agaagaggtc
      661 ccgaaatcgc ggccctccac catcctgggg gcgccgacca agggacgatt accgacgccg
      721 ctctcctccc cctcgaagac gagtcactat aatgagcctt cttacaacgc tgtgaggatc
      781 cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
      841 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
      901 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
      961 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     1021 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     1081 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     1141 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     1201 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     1261 tcgttgaccg aatcaccgac ctctctcccc agcaattcac ccgcgcgcgg gaactaaacc
     1321 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     1381 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     1441 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     1501 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     1561 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     1621 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     1681 cgtgaattgc tgccctctgg ttatgtgtgg gagggcttcg aaggaagcgg agaaggtaga
     1741 ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc tagactggac
     1801 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
     1861 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     1921 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     1981 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     2041 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     2101 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     2161 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     2221 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     2281 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     2341 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     2401 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     2461 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     2521 ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact atgttgctcc
     2581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2881 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     2941 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg tttcgcctcg
     3121 gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3181 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3241 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3301 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3361 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3421 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     3481 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3541 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3601 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3661 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3721 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3781 agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg agacaataac
     3841 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3901 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3961 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4021 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4081 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4141 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4201 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4261 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4321 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4381 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4441 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4501 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4561 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4621 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4681 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4741 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4801 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4861 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4921 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4981 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5041 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5101 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5161 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5221 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5281 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5341 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5401 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5461 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5521 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5581 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5641 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5701 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5761 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5821 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5881 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     5941 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6001 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6061 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6121 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6181 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6361 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6421 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     6481 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     6541 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     6601 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     6661 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     6721 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     6781 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     6841 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     6901 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6961 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7021 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7081 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7141 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7201 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7261 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7321 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7381 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7441 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7501 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7561 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7621 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacaattctc
     7681 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     7741 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     7801 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacaggga
//
