LOCUS       sequence-338281-        1765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mini plasmid containing minimal sequences for amplification in 
            bacteria and two BbvCI nicking sites for introduction of modified 
            DNA through annealing and ligation (e.g. mismatch, DNA lesions, 
            flap)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Fernandez-Leiro R, Bhairosing-Kok D, Kunetsky V, Laffeber C, 
            Winterwerp HH, Groothuizen F, Fish A, Lebbink JHG, Friedhoff P, 
            Sixma TK, Lamers MH
  TITLE     The selection process of licensing a DNA mismatch for repair.
  JOURNAL   Nat Struct Mol Biol. 2021 Apr;28(4):373-381. doi: 
            10.1038/s41594-021-00577-7. Epub 2021 Apr 5.
  PUBMED    33820992
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-021-00577-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2021-04"; volume: "28"; issue: "4"; pages: "373-381"
COMMENT     Sequence Label: pRC1765
FEATURES             Location/Qualifiers
     source          1..1765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(50..638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(130..149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(809..1600,1601..1669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1432..1451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(1670..1765,1..9))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggttccgcgc ctcagcatca ttgctaccca taatgtgcgg tacctcagct ttccataggc
       61 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      121 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      181 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      241 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      301 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      361 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      421 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      481 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      541 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      601 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      661 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      721 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      781 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      841 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      901 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      961 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1021 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1081 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1141 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1201 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1261 aaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1321 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1381 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1441 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg ggtcgaaaac
     1501 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1561 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1621 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1681 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1741 gtatttagaa aaataaacaa atagg
//
