LOCUS       sequence-338283-        4272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC41A2_STOP
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1829..1928)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1945..1964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1946..1964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1969..1985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1969..1985)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2087..2106)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2098..2907
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2165..2184)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3543..3562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3796..3813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3972..3999)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4091..4177)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4226..4248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4240..4257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4241..4257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgac caacagcaag
      121 ggccggtcca tcaccgataa gacaagcgga ggacctagct ccggaggagg attcgtggac
      181 tggaccctga gactgaacac aatccagagc gataagtttc tgaatctgct gctgtccatg
      241 gtgccagtga tctatcagaa gaaccaggag gacaggcaca agaaggccaa tggcatctgg
      301 caggatggcc tgtccaccgc cgtgcagaca ttcagcaatc gctccgagca gcacatggag
      361 taccactctt tcagcgagca gtcttttcac gccaacaatg gccacgcctc tagctcctgc
      421 agccagaagt atgacgatta cgccaactat aattactgtg atggcaggga gaccagcgag
      481 accacagcca tgctgcagga cgaggatatc tctagcgacg gcgatgagga cgccatcgtg
      541 gaggtgacac ccaagctgcc taaggagtcc tctggcatca tggccctgca gatcctggtg
      601 cctttcctgc tggcaggatt tggaaccgtg tccgccggaa tggtgctgga catcgtgcag
      661 cactgggagg tgttccggaa ggtgacagag gtgtttatcc tggtgccagc cctgctgggc
      721 ctgaagggca acctggagat gaccctggcc tctagactga gcacagccgt gaatatcggc
      781 aagatggata gccccatcga gaagtggaac ctgatcatcg gcaatctggc cctgaagcag
      841 gtgcaggcca ccgtggtggg cttcctggca gcagtggcag caatcatcct gggctggatc
      901 cccgagggca agtactatct ggaccactcc atcctgctgt gcagctcctc tgtggccaca
      961 gcctttatcg cctccctgct gcagggcatc atcatggtgg gcgtgatcgt gggctctaag
     1021 aagaccggca tcaaccctga caatgtggcc acaccaatcg ccgcctcttt cggcgatctg
     1081 atcaccctgg ccatcctggc ctggatctcc cagggcctgt attcttgtct ggagacctac
     1141 tattacatca gccccctggt gggcgtgttc tttctggccc tgacacctat ctggatcatc
     1201 atcgcagcaa agcacccagc aaccaggaca gtgctgcaca gcggatggga gcctgtgatc
     1261 accgccatgg tcatcagctc catcggcggc ctgatcctgg acaccacagt gtccgatccc
     1321 aacctggtgg gcatcgtggt gtatacccct gtgatcaacg gcatcggcgg caatctggtg
     1381 gccatccagg cctccaggat ctctacatac ctgcacctgc actccatccc aggagagctg
     1441 ccagacgagc caaagggatg ctattaccca ttccgcacct tctttggccc cggcgtgaac
     1501 aataagtctg cccaggtgct gctgctgctg gtcatccccg gccacctgat ctttctgtat
     1561 acaatccacc tgatgaagtc tggccacacc agcctgacaa tcatcttcat cgtggtgtac
     1621 ctgtttggcg ccgtgctgca ggtgtttacc ctgctgtgga tcgccgactg gatggtgcac
     1681 cacttctggc ggaagggcaa ggatcccgac tccttttcta tcccttacct gaccgccctg
     1741 ggcgatctgc tgggaacagc cctgctggcc ctgagcttcc actttctgtg gctgatcggc
     1801 gatagagacg gcgatgtggg cgactaagac ccagctttct tgtacaaagt tggcattata
     1861 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1921 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1981 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     2041 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     2101 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     2161 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     2221 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2281 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     2341 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     2401 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2461 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2521 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2581 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2641 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2701 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2761 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2821 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2881 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2941 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     3001 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3901 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3961 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     4021 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     4081 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     4141 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     4201 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     4261 aagctcgggc cc
//
