LOCUS       sequence-338287-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC5A7_STOP
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1850..1949)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1966..1985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1967..1985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1990..2006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1990..2006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2108..2127)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2119..2928
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2186..2205)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3075..3663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3564..3583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3817..3834
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3993..4020)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4112..4198)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4247..4269
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4261..4278
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4262..4278
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cttccacgtg
      121 gagggcctga tcgccatcat cgtgttttac ctgctgatcc tgctggtggg aatctgggca
      181 gcatggagga caaagaacag cggatccgcc gaggagagga gcgaggcaat catcgtggga
      241 ggaagggaca tcggactgct ggtgggcggc ttcaccatga cagcaacctg ggtgggagga
      301 ggctacatca atggaaccgc agaggccgtg tacgtgccag gatatggact ggcatgggca
      361 caggcaccta tcggatattc tctgagcctg atcctgggcg gcctgttctt tgccaagcca
      421 atgcggtcca agggctacgt gaccatgctg gatcccttcc agcagatcta tggcaagaga
      481 atgggcggcc tgctgtttat ccctgccctg atgggcgaga tgttctgggc agcagcaatc
      541 tttagcgccc tgggagccac aatctccgtg atcatcgacg tggatatgca catctccgtg
      601 atcatctctg ccctgatcgc cacactgtac accctggtgg gaggactgta cagcgtggcc
      661 tataccgacg tggtgcagct gttctgcatc tttgtgggcc tgtggatctc tgtgcccttc
      721 gccctgagcc accctgcagt ggccgatatc ggctttacag ccgtgcacgc caagtaccag
      781 aagccatggc tgggaaccgt ggacagctcc gaggtgtata gctggctgga ttccttcctg
      841 ctgctgatgc tgggcggcat cccttggcag gcctactttc agcgggtgct gtctagctcc
      901 tctgccacct atgcccaggt gctgtctttc ctggccgcct ttggctgcct ggtcatggcc
      961 atcccagcaa tcctgatcgg agcaatcgga gccagcacag actggaacca gaccgcctac
     1021 ggcctgccag accccaagac cacagaggag gccgatatga tcctgcctat cgtgctgcag
     1081 tacctgtgcc cagtgtatat ctctttcttt ggactgggag ccgtgagcgc cgccgtgatg
     1141 agctccgccg actctagcat cctgagcgcc tcctctatgt tcgcccggaa catctaccag
     1201 ctgtccttta gacagaatgc ctctgataag gagatcgtgt gggtcatgcg gatcacagtg
     1261 ttcgtgtttg gagcatccgc caccgcaatg gcactgctga caaagaccgt gtacggcctg
     1321 tggtatctga gctccgatct ggtgtatatc gtgatcttcc cacagctgct gtgcgtgctg
     1381 tttgtgaagg gcacaaatac ctacggagca gtggcaggat acgtgagcgg actgttcctg
     1441 agaatcacag gcggcgagcc atacctgtat ctgcagcccc tgatctttta ccctggctac
     1501 tatccagacg ataacggcat ctataatcag aagttcccct ttaagaccct ggccatggtg
     1561 acatccttcc tgaccaacat ctgtatctct tacctggcca agtatctgtt cgagagcggc
     1621 acactgcccc ctaagctgga cgtgtttgat gccgtggtgg ccaggcactc cgaggagaat
     1681 atggacaaga ccatcctggt gaagaacgag aatatcaagc tggatgagct ggccctggtg
     1741 aagcctcgcc agtccatgac actgtctagc acattcacca acaaggaggc ctttctggac
     1801 gtggattcct ctccagaggg ctctggcacc gaggacaatc tgcagtaaga cccagctttc
     1861 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1921 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1981 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2041 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2101 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2161 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2221 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2281 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2341 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2401 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2461 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2521 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2581 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2641 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2701 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2761 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2821 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2881 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2941 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3001 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3841 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3901 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3961 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4021 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4081 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4141 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4201 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4261 tgtaaaacga cggccagtct taagctcggg ccc
//
